About: Detection of HIV cDNA Point Mutations with Rolling-Circle Amplification Arrays

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of HIV cDNA Point Mutations with Rolling-Circle Amplification Arrays
Creator	Liu, Quanjun Lu, Zuhong Wu, Lingwei Wu, Zhongwei
Source	Medline; PMC
abstract	In this paper we describe an isothermal rolling-circle amplification (RCA) protocol to detect gene point mutations on chips. The method is based on an allele-specific oligonucleotide circularization mediated by a special DNA ligase. The probe is circularized when perfect complementary sequences between the probe oligonucleotide and HIV cDNA gene. Mismatches around the ligation site can prevent probe circularization. The circularized probe (C-probe) can be amplified by rolling circle amplification to generate multimeric singlestranded DNA (ssDNA) under isothermal conditions. There are four sequence regions to bind respectively with fluorescent probe, RCA primer, solid probe and HIV cDNA template in the C-probe which we designed. These ssDNA products are hybridized with fluorescent probes and solid probes which are immobilized on a glass slide composing a regular microarray pattern. The fluorescence signals can be monitored by a scanner in the presence of HIV cDNA templates, whereas the probes cannot be circularized and signal of fluorescence cannot be found. The RCA array has capability of high-throughput detection of the point mutation and the single-nucleotide polymorphism (SNP). The development of C-probe-based technologies offers a promising prospect for situ detection, microarray, molecular diagnosis, single nucleotide polymorphism, and whole genome amplification.
has issue date	2010-01-27(xsd:dateTime)
bibo:doi	10.3390/molecules15020619
bibo:pmid	20335932
has license	cc-by
sha1sum (hex)	0539cdf9c5215f7d1f53d1cb7af556ba1f799937
schema:url	https://doi.org/10.3390/molecules15020619
resource representing a document's title	Detection of HIV cDNA Point Mutations with Rolling-Circle Amplification Arrays
has PubMed Central identifier	PMC6256933
has PubMed identifier	20335932
schema:publication	Molecules
resource representing a document's body	covid:0539cdf9c5215f7d1f53d1cb7af556ba1f799937#body_text
is schema:about of	named entity 'presence' named entity 'circularization' named entity 'microarray' named entity 'molecules' named entity 'Amplification' covid:arg/0539cdf9c5215f7d1f53d1cb7af556ba1f799937 named entity 'RCA' named entity 'slide' named entity 'multimeric' named entity 'ssDNA' named entity 'gene' named entity 'ligation' named entity 'mediated' named entity 'special' named entity 'fluorescent' named entity 'conditions' named entity 'circularized' named entity 'complementary sequences' named entity 'hybridized' named entity 'cDNA' named entity 'ssDNA' named entity 'fluorescence' named entity 'glass slide' named entity 'point mutation' named entity 'gene' named entity 'rolling circle amplification' named entity 'primer' named entity 'genome' named entity 'cDNA' named entity 'allele-specific oligonucleotide' named entity 'HIV' named entity 'cDNA' named entity 'Rolling-Circle' named entity 'HIV' named entity 'HIV' named entity 'ligation' named entity 'incubation' named entity 'point mutation' named entity 'oligonucleotide' named entity 'nucleic acid sequences' named entity 'covalent bonds' named entity 'rolling circle amplification' named entity 'biological assays' named entity 'HIV' named entity 'SNP' named entity 'PCR' named entity 'phenotypes' named entity 'hybrids' named entity 'RCA' named entity 'polymerase chain reaction' named entity 'acetone' named entity 'ligated' named entity 'microarray' named entity 'HIV' named entity 'rolling-circle' named entity 'aldehydes' named entity 'phi29 DNA polymerase' named entity 'RCA' named entity 'double-helical' named entity 'glutaraldehyde' named entity 'point mutations' named entity 'phi29 DNA polymerase' named entity 'polymerase' named entity 'RCA' named entity 'SDS' named entity 'DNA arrays' named entity 'microarray hybridization' named entity 'Bovine Serum Albumin' named entity 'genomes' named entity 'oligonucleotide probes' named entity 'phi29 DNA polymerase' named entity 'RCA' named entity 'Tecan' named entity 'deoxynucleoside triphosphate' named entity 'single-stranded' named entity 'phi29 DNA polymerase' named entity 'phi29 DNA polymerase' named entity 'pH 9'

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