About: Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus

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About: Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus
Creator	Wang, Li Jiang, Yanping Li, Yijing Qiao, Xinyuan Tang, Lijie Xu, Yigang Huang, Xuewei Ma, Sunting Wang, Xiaona Shi, Wen Chen, Su
Source	Medline; PMC
abstract	BACKGROUND: Porcine epidemic diarrhea caused by porcine epidemic diarrhea virus (PEDV) has led to serious economic losses to the swine industry worldwide. In this study, an oral recombinant Lactobacillus casei vaccine against PEDV infection targeting the intestinal microfold (M) cells and dendritic cells (DCs) for delivering the core neutralizing epitope (COE) of PEDV spike protein was developed with M cell-targeting peptide (Col) and dendritic cell-targeting peptide (DCpep). The immunogenicity of the orally administered recombinant strains was evaluated. RESULTS: After immunization, significantly higher levels of anti-PEDV specific IgG antibodies with PEDV neutralizing activity in the sera and mucosal sIgA antibodies in the tractus genitalis, intestinal mucus, and stools were detected in mice orally administered with the recombinant strain pPG-COE-Col-DCpep/L393, which expressed DCpep and Col targeting ligands fused with the PEDV COE antigen, compared to mice orally immunized with the recombinant strain pPG-COE/L393 without the DCpep and Col targeting ligands. Moreover, in response to restimulation with the PEDV COE antigen in vitro, a significant difference in splenocyte proliferation response and Th2-associated cytokine IL-4 level was observed in the group of mice orally immunized with pPG-COE-Col-DCpep/L393 (p < 0.05) compared to the groups of mice that received pPG-COE-Col/L393 and pPG-COE-DCpep/L393, respectively. CONCLUSIONS: The intestinal M cells- and DCs-targeting oral delivery of genetically engineered Lactobacillus expressing the COE antigen of PEDV can efficiently induce anti-PEDV mucosal, humoral, and cellular immune responses via oral administration, suggesting a promising vaccine strategy against PEDV infection. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12934-018-0861-7) contains supplementary material, which is available to authorized users.
has issue date	2018-02-09(xsd:dateTime)
bibo:doi	10.1186/s12934-018-0861-7
bibo:pmid	29426335
has license	cc-by
sha1sum (hex)	08467794d024d78c7981f7c39743a9faaa1adecb
schema:url	https://doi.org/10.1186/s12934-018-0861-7
resource representing a document's title	Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus
has PubMed Central identifier	PMC5807822
has PubMed identifier	29426335
schema:publication	Microb Cell Fact
resource representing a document's body	covid:08467794d024d78c7981f7c39743a9faaa1adecb#body_text
is schema:about of	named entity 'TARGETING' named entity 'ORAL' named entity 'Lactobacillus casei' named entity 'targeting' named entity 'oral' named entity 'peptide' named entity 'led' named entity 'intestinal' named entity 'Lactobacillus' named entity 'INTESTINAL' named entity 'RECOMBINANT' named entity 'MICROBIAL' named entity 'M CELL' named entity 'SWINE' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'PEPTIDE' named entity 'CAUSED BY' named entity 'CELLS' named entity 'STUDY' named entity 'ECONOMIC' named entity 'BACKGROUND' named entity 'TARGETING' named entity 'DENDRITIC CELL' named entity 'EVALUATED' named entity 'ADMINISTERED' named entity 'IMMUNOGENICITY' named entity 'PORCINE EPIDEMIC DIARRHEA' named entity 'MICROFOLD' named entity 'CORE' named entity 'PROTEIN ' named entity 'ORAL' named entity 'VACCINE' named entity 'CORE' named entity 'RECOMBINANT' named entity 'LACTOBACILLUS CASEI' named entity 'CELL' named entity 'EPITOPE' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'OPEN' named entity 'CELLS' named entity 'VACCINE' named entity 'INTESTINAL' named entity 'SPIKE PROTEIN' named entity 'STRAINS' named entity 'ACCESS' named entity 'FACTORIES' named entity 'DENDRITIC CELLS' named entity 'MICROFOLD' named entity 'LACTOBACILLUS' named entity 'DENDRITIC CELLS' named entity 'WORLDWIDE' named entity 'EPITOPE' named entity 'INDUSTRY' named entity 'INFECTION' named entity 'LED' covid:arg/08467794d024d78c7981f7c39743a9faaa1adecb named entity 'epidemic' named entity 'immunogenicity' named entity 'Microbial' named entity 'peptide' named entity 'dendritic cells' named entity 'epidemic' named entity 'recombinant strains' named entity 'porcine epidemic diarrhea virus' named entity 'Microbial Cell Factories' named entity 'recombinant' named entity 'epitope' named entity 'Lactobacillus' named entity 'serum' named entity 'antigen' named entity 'genital tract'

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