About: Rapid detection of MERS coronavirus-like viruses in bats: potential for tracking MERS coronavirus transmission and animal origin

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About: Rapid detection of MERS coronavirus-like viruses in bats: potential for tracking MERS coronavirus transmission and animal origin Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Rapid detection of MERS coronavirus-like viruses in bats: potential for tracking MERS coronavirus transmission and animal origin
Creator	Yuen, Kwok-Yung Chan, Kwok-Hung Xia, Ningshao Chen, Honglin Chen, Yixin Zhang, Libiao Lau, Susanna Wong, Emily Woo, Patrick
source	Medline; PMC
abstract	Recently, we developed a monoclonal antibody-based rapid nucleocapsid protein detection assay for diagnosis of MERS coronavirus (MERS-CoV) in humans and dromedary camels. In this study, we examined the usefulness of this assay to detect other lineage C betacoronaviruses closely related to MERS-CoV in bats. The rapid MERS-CoV nucleocapsid protein detection assay was tested positive in 24 (88.9%) of 27 Tylonycteris bat CoV HKU4 (Ty-BatCoV-HKU4) RNA-positive alimentary samples of Tylonycteris pachypus and 4 (19.0%) of 21 Pipistrellus bat CoV HKU5 (Pi-BatCoV-HKU5) RNA-positive alimentary samples of Pipistrellus abramus. There was significantly more Ty-BatCoV-HKU4 RNA-positive alimentary samples than Pi-BatCoV-HKU5 RNA-positive alimentary samples that were tested positive by the rapid MERS-CoV nucleocapsid protein detection assay (P < 0.001 by Chi-square test). The rapid assay was tested negative in all 51 alimentary samples RNA-positive for alphacoronaviruses (Rhinolophus bat CoV HKU2, Myotis bat CoV HKU6, Miniopterus bat CoV HKU8 and Hipposideros batCoV HKU10) and 32 alimentary samples positive for lineage B (SARS-related Rhinolophus bat CoV HKU3) and lineage D (Rousettus bat CoV HKU9) betacoronaviruses. No significant difference was observed between the viral loads of Ty-BatCoV-HKU4/Pi-BatCoV-HKU5 RNA-positive alimentary samples that were tested positive and negative by the rapid test (Mann-Witney U test). The rapid MERS-CoV nucleocapsid protein detection assay is able to rapidly detect lineage C betacoronaviruses in bats. It detected significantly more Ty-BatCoV-HKU4 than Pi-BatCoV-HKU5 because MERS-CoV is more closely related to Ty-BatCoV-HKU4 than Pi-BatCoV-HKU5. This assay will facilitate rapid on-site mass screening of animal samples for ancestors of MERS-CoV and tracking transmission in the related bat species.
has issue date	2018-03-07(xsd:dateTime)
bibo:doi	10.1038/s41426-017-0016-7
bibo:pmid	29511173
has license	cc-by
sha1sum (hex)	08e6a1b558bcb3962378851963c2e67681dabad6
schema:url	https://doi.org/10.1038/s41426-017-0016-7
resource representing a document's title	Rapid detection of MERS coronavirus-like viruses in bats: potential for tracking MERS coronavirus transmission and animal origin
has PubMed Central identifier	PMC5841240
has PubMed identifier	29511173
schema:publication	Emerg Microbes Infect
resource representing a document's body	covid:08e6a1b558bcb3962378851963c2e67681dabad6#body_text
is schema:about of	named entity 'ORIGIN' named entity 'ANIMAL' named entity 'nucleocapsid' named entity 'tested' named entity 'tested' named entity 'alimentary' named entity 'MERS-CoV' named entity 'samples' named entity 'RAPID' named entity 'BASED' named entity 'MERS CORONAVIRUS' named entity 'VIRAL' named entity 'POSITIVE' named entity 'MERS-COV' named entity 'LINEAGE' named entity 'OBSERVED' named entity 'FOR DIAGNOSIS' named entity 'RAPID' named entity 'STUDY' named entity 'TO DETECT' named entity 'NEGATIVE' named entity 'RNA' named entity 'WITNEY' named entity '28P' named entity 'RELATED' named entity 'ASSAY' named entity 'DROMEDARY' named entity 'TEST' named entity 'USEFULNESS' named entity 'EXAMINED' named entity 'BAT COV HKU9' named entity 'TRACKING' named entity 'MERS CORONAVIRUS' named entity 'HUMANS' named entity 'TYLONYCTERIS PACHYPUS' named entity 'SAMPLES' named entity 'NUCLEOCAPSID PROTEIN' named entity 'RECENTLY' named entity 'LIKE' named entity 'BATS' named entity 'VIRUSES' named entity 'DETECTION' named entity 'PROTEIN DETECTION' named entity 'MINIOPTERUS' named entity 'PIPISTRELLUS ABRAMUS' named entity 'CHI-SQUARE TEST' named entity 'BETACORONAVIRUSES' named entity 'CAMELS' named entity 'BATS' named entity 'HIPPOSIDEROS' named entity 'MYOTIS' named entity 'PIPISTRELLUS' named entity 'TESTED' named entity 'RHINOLOPHUS' named entity 'POSITIVE AND NEGATIVE' named entity 'TYLONYCTERIS' named entity 'SIGNIFICANT' named entity 'POTENTIAL' named entity 'BAT' named entity 'MONOCLONAL ANTIBODY' named entity '0.001' named entity 'DIFFERENCE' covid:arg/08e6a1b558bcb3962378851963c2e67681dabad6 named entity 'samples' named entity 'Hipposideros' named entity 'samples' named entity 'nucleocapsid' named entity 'MERS coronavirus' named entity 'monoclonal antibody' named entity 'bat' named entity 'Pipistrellus' named entity 'assay' named entity 'dromedary'

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