About: Cathepsin B & L Are Not Required for Ebola Virus Replication

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Cathepsin B & L Are Not Required for Ebola Virus Replication
Creator	Reinheckel, Thomas Feldmann, Heinz Marzi, Andrea
source	Medline; PMC
abstract	Ebola virus (EBOV), family Filoviridae, emerged in 1976 on the African continent. Since then it caused several outbreaks of viral hemorrhagic fever in humans with case fatality rates up to 90% and remains a serious Public Health concern and biothreat pathogen. The most pathogenic and best-studied species is Zaire ebolavirus (ZEBOV). EBOV encodes one viral surface glycoprotein (GP), which is essential for replication, a determinant of pathogenicity and an important immunogen. GP mediates viral entry through interaction with cellular surface molecules, which results in the uptake of virus particles via macropinocytosis. Later in this pathway endosomal acidification activates the cysteine proteases Cathepsin B and L (CatB, CatL), which have been shown to cleave ZEBOV-GP leading to subsequent exposure of the putative receptor-binding and fusion domain and productive infection. We studied the effect of CatB and CatL on in vitro and in vivo replication of EBOV. Similar to previous findings, our results show an effect of CatB, but not CatL, on ZEBOV entry into cultured cells. Interestingly, cell entry by other EBOV species (Bundibugyo, Côte d'Ivoire, Reston and Sudan ebolavirus) was independent of CatB or CatL as was EBOV replication in general. To investigate whether CatB and CatL have a role in vivo during infection, we utilized the mouse model for ZEBOV. Wild-type (control), catB(−/−) and catL(−/−) mice were equally susceptible to lethal challenge with mouse-adapted ZEBOV with no difference in virus replication and time to death. In conclusion, our results show that CatB and CatL activity is not required for EBOV replication. Furthermore, EBOV glycoprotein cleavage seems to be mediated by an array of proteases making targeted therapeutic approaches difficult.
has issue date	2012-12-06(xsd:dateTime)
bibo:doi	10.1371/journal.pntd.0001923
bibo:pmid	23236527
has license	cc0
sha1sum (hex)	0b05d3ede3351f8b3edb22c74b0cfe933ba408c1
schema:url	https://doi.org/10.1371/journal.pntd.0001923
resource representing a document's title	Cathepsin B & L Are Not Required for Ebola Virus Replication
has PubMed Central identifier	PMC3516577
has PubMed identifier	23236527
schema:publication	PLoS Negl Trop Dis
resource representing a document's body	covid:0b05d3ede3351f8b3edb22c74b0cfe933ba408c1#body_text
is schema:about of	named entity 'CATHEPSIN B' named entity 'time' named entity 'Bundibugyo' named entity 'African continent' named entity 'Cathepsin B' named entity 'VIRUS REPLICATION' covid:arg/0b05d3ede3351f8b3edb22c74b0cfe933ba408c1 named entity 'PREVIOUS FINDINGS' named entity '2C CELL' named entity 'LEADING' named entity 'SIMILAR' named entity 'OUTBREAKS' named entity 'ARRAY' named entity 'PATHOGEN' named entity 'TARGETED' named entity 'THERAPEUTIC' named entity 'CHALLENGE' named entity 'CYSTEINE PROTEASES' named entity 'EBOLA VIRUS' named entity 'CATL' named entity 'DOMAIN' named entity 'PRODUCTIVE' named entity 'TO INVESTIGATE' named entity 'HAVE' named entity 'MACROPINOCYTOSIS' named entity 'INTERACTION' named entity 'PATHOGENICITY' named entity 'ADAPTED' named entity 'CASE' named entity 'ROLE' named entity 'RATES' named entity 'UP TO' named entity 'RESTON' named entity 'NOT REQUIRED' named entity 'MOUSE MODEL' named entity 'VIRAL ENTRY' named entity 'REPLICATION' named entity 'MOLECULES' named entity 'MEDIATED' named entity 'RECEPTOR' named entity 'VIRAL' named entity 'CATHEPSIN B' named entity 'TO CLEAVE' named entity 'CONCLUSION' named entity 'SPECIES' named entity 'RESULTS' named entity 'DETERMINANT' named entity 'PATHWAY' named entity 'CAUSED' named entity 'PUBLIC HEALTH' named entity 'ZAIRE EBOLAVIRUS' named entity 'CATB' named entity 'ACTIVITY' named entity 'CONCERN' named entity 'UPTAKE' named entity 'IN VIVO' named entity 'WILD-TYPE' named entity 'CULTURED CELLS' named entity 'ENTRY' named entity 'ACIDIFICATION' named entity 'APPROACHES' named entity 'VIRUS PARTICLES' named entity 'DIFFICULT' named entity 'ESSENTIAL' named entity 'EBOLA VIRUS' named entity 'NOT REQUIRED' named entity 'SUBSEQUENT' named entity 'SUSCEPTIBLE' named entity 'LETHAL' named entity 'ACTIVATES' named entity 'OUR' named entity 'IMPORTANT' named entity 'LATER' named entity 'NO DIFFERENCE' named entity 'IN VITRO' named entity 'VIRUS REPLICATION' named entity 'BEST' named entity 'MOUSE' named entity 'TIME TO DEATH'

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