About: Expression of coronavirus E1 and rotavirus VP10 membrane proteins from synthetic RNA

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About: Expression of coronavirus E1 and rotavirus VP10 membrane proteins from synthetic RNA Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Expression of coronavirus E1 and rotavirus VP10 membrane proteins from synthetic RNA
Creator	Armstrong, John Colman, Alan Mccrae, Malcolm
Source	Medline; PMC
abstract	Some viruses acquire their envelopes by budding through internal membranes of their host cell. We have expressed the cloned cDNA for glycoproteins from two such viruses, the E1 protein of coronavirus, which buds in the Golgi region, and VP10 protein of rotavirus, which assembles in the endoplasmic reticulum. Messenger RNA was prepared from both cDNAs by using SP6 polymerase and either translated in vitro or injected into cultured CV1 cells or Xenopus oocytes. In CV1 cells, the El protein was localised to the Golgi region and VP10 protein to the endoplasmic reticulum. In Xenopus oocytes, the E1 protein acquired post‐translational modifications indistinguishable from the sialylated, O‐linked sugars found on viral protein, while the VP10 protein acquired endoglycosidase‐H‐sensitive N‐linked sugars, consistent with their localisation to the Golgi complex and endoplasmic reticulum, respectively. Thus the two proteins provide models with which to study targeting to each of these intracellular compartments. When the RNAs were expressed in matured, meiotic oocytes, the VP10 protein was modified as before, but the E1 protein was processed to a much lesser extent than in interphase oocytes, consistent with a cessation of vesicular transport during cell division.
has issue date	2004-02-19(xsd:dateTime)
bibo:doi	10.1002/jcb.240350206
bibo:pmid	2448319
has license	no-cc
sha1sum (hex)	0ba56d8d00d9a7761ca1110f0ee1ba330cef767b
schema:url	https://doi.org/10.1002/jcb.240350206
resource representing a document's title	Expression of coronavirus E1 and rotavirus VP10 membrane proteins from synthetic RNA
has PubMed Central identifier	PMC7166569
has PubMed identifier	2448319
schema:publication	J Cell Biochem
resource representing a document's body	covid:0ba56d8d00d9a7761ca1110f0ee1ba330cef767b#body_text
is schema:about of	named entity 'extent' named entity 'coronavirus' named entity 'cDNAs' named entity 'indistinguishable' named entity 'Golgi complex' named entity 'protein' named entity 'Membrane' named entity 'protein' named entity 'injected' named entity 'cell division' named entity 'cultured' named entity 'rotavirus' named entity 'host cell' named entity 'cloned' named entity 'intracellular compartments' named entity 'glycoproteins' named entity 'cDNAs' named entity 'protein' named entity 'meiotic' named entity 'RNA' named entity 'envelopes' named entity 'reticulocyte' named entity 'protein' named entity 'reticulocyte' named entity 'immunofluorescence' named entity 'BglII' named entity 'Golgi' named entity 'cloned' named entity 'Xenopus oocytes' named entity 'secretory pathway' named entity 'Golgi apparatus' named entity 'Golgi complex' named entity 'immunofluorescence' named entity 'viral infection' named entity 'reticulocyte' named entity 'Golgi complex' named entity 'virion' named entity 'endoplasmic reticulum' named entity '0.5mM' named entity 'Incubation' named entity 'membrane proteins' named entity 'meiotic' named entity 'nucleotides' named entity 'oligosaccharides' named entity 'Golgi complex' named entity 'serum' named entity 'daughter cells' named entity 'protein' named entity 'viral protein' named entity 'molecule' named entity 'morphogenesis' named entity 'plasma membrane' named entity 'lysates' named entity 'glycoprotein' named entity 'oocytes' named entity 'membrane traffic' named entity 'protein' named entity 'hydrophobic regions' named entity 'cell surface' named entity 'protein' named entity 'vector' named entity 'endoplasmic reticulum' named entity 'rough endoplasmic reticulum' named entity 'virus' named entity 'endoplasmic reticulum' named entity 'cDNA' named entity 'Golgi complex' named entity 'endoplasmic reticulum' named entity 'similar function' named entity 'fluorescence microscopy' named entity 'endoplasmic reticulum' named entity 'endoglycosidase' named entity 'microinjected' named entity 'post-translational modifications' named entity 'Rotavirus' named entity 'nucleotides' named entity 'endoplasmic reticulum' named entity 'BglII' named entity 'secretory pathway'

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