About: Activity of a purified His-tagged 3C-like proteinase from the coronavirus infectious bronchitis virus

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About: Activity of a purified His-tagged 3C-like proteinase from the coronavirus infectious bronchitis virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Activity of a purified His-tagged 3C-like proteinase from the coronavirus infectious bronchitis virus
Creator	Cavanagh, D Brown, T Tibbles, K
Source	Elsevier; Medline; PMC
abstract	Abstract Previous studies in vitro of the processing of cloned polyprotein fragments from the coronavirus infectious bronchitis virus (IBV) large open reading frame (ORF1), confirmed the activity of a predicted 3C-like proteinase (3CLP) domain and suggested that the proteinase is released autocatalytically from the polyprotein in the form of a 35 kDa protein, 3CLpro, capable of further cleavages in trans. In order to identify such cleavages within the ORF1 polyprotein mediated by 3CLpro, the proteinase was expressed in bacteria, purified and used in trans cleavage assays with polyprotein fragments lacking the 3CLP domain as targets. The proteinase was expressed as a polyprotein fragment which was able to process during expression in bacterial cells, releasing mature 3CLpro. A histidine (His6) tag was introduced close to the C-terminus of the proteinase to aid purification. Processing demonstrated by the tagged proteinase was indistinguishable from that of the wild-type enzyme indicating that the site chosen for the tag was permissive. From these studies we were able to demonstrate trans cleavages consistent with the use of most of the previously predicted or identified sites within the open reading frame of gene 1. This tentatively completes the processing map for the ORF1 region with respect to 3CLpro.
has issue date	1999-04-30(xsd:dateTime)
bibo:doi	10.1016/s0168-1702(99)00011-8
bibo:pmid	10392722
has license	els-covid
sha1sum (hex)	0d53a8766b1807b6a4ddf41206978f5b1824b324
schema:url	https://doi.org/10.1016/s0168-1702%2899%2900011-8
resource representing a document's title	Activity of a purified His-tagged 3C-like proteinase from the coronavirus infectious bronchitis virus
has PubMed Central identifier	PMC7126649
has PubMed identifier	10392722
schema:publication	Virus Research
resource representing a document's body	covid:0d53a8766b1807b6a4ddf41206978f5b1824b324#body_text
is schema:about of	named entity 'tag' named entity 'cleavages' named entity 'predicted' named entity 'cleavage' named entity 'This' named entity 'proteinase' named entity 'virus' named entity 'Activity' named entity 'DEMONSTRATED' named entity 'MEDIATED' named entity 'LACKING' named entity 'LARGE' named entity 'USED' named entity 'WILD-TYPE' named entity 'TARGETS' named entity 'CONSISTENT WITH' named entity 'ACTIVITY' named entity 'PROCESS' named entity 'POLYPROTEIN FRAGMENT' named entity 'INTRODUCED' named entity 'CHOSEN' named entity 'REGION' named entity 'TAG' named entity 'SITE' named entity 'FORM' named entity 'PREDICTED' named entity 'CLOSE TO' named entity 'IN VITRO' named entity 'EXPRESSION' named entity 'PURIFIED' named entity 'PREVIOUS' named entity 'PROTEINASE' named entity 'LIKE' named entity 'EXPRESSED' named entity 'ACTIVITY' named entity 'TAGGED' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'CORONAVIRUS' named entity 'HIS-' named entity 'LIKE' named entity 'AID' named entity 'FRAGMENTS' named entity 'PURIFICATION' named entity 'PROCESSING' named entity 'MATURE' named entity 'STUDIES' named entity 'BACTERIAL' named entity 'PURIFIED' named entity 'CORONAVIRUS' named entity 'TO IDENTIFY' named entity 'CLONED' named entity 'OPEN READING FRAME' named entity 'CELLS' named entity 'DOMAIN' named entity 'C-TERMINUS' named entity 'ORF1' named entity 'HISTIDINE' named entity 'INDICATING' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'RESPECT' named entity 'USE OF' named entity 'BACTERIA' named entity 'PROTEIN' named entity 'RELEASING' named entity 'CLEAVAGE' named entity 'CAPABLE' named entity 'IN TRANS' named entity 'RELEASED' named entity 'MAP' named entity 'ENZYME' named entity 'THESE' named entity 'IDENTIFIED' named entity 'CONFIRMED' named entity 'KDA' named entity 'TAGGED' named entity 'POLYPROTEIN' named entity 'TRANS' named entity 'PROTEINASE' named entity 'ORDER'

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