About: Transcriptome Analysis of Human Peripheral Blood Mononuclear Cells Exposed to Lassa Virus and to the Attenuated Mopeia/Lassa Reassortant 29 (ML29), a Vaccine Candidate

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About: Transcriptome Analysis of Human Peripheral Blood Mononuclear Cells Exposed to Lassa Virus and to the Attenuated Mopeia/Lassa Reassortant 29 (ML29), a Vaccine Candidate Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Transcriptome Analysis of Human Peripheral Blood Mononuclear Cells Exposed to Lassa Virus and to the Attenuated Mopeia/Lassa Reassortant 29 (ML29), a Vaccine Candidate
Creator	Carrion, R Crasta, O Patterson, J Zapata, J Zhang, Y
Source	PMC
abstract	Lassa virus (LASV) is the causative agent of Lassa Fever and is responsible for several hundred thousand infections and thousands of deaths annually in West Africa. LASV and the non-pathogenic Mopeia virus (MOPV) are both rodent-borne African arenaviruses. A live attenuated reassortant of MOPV and LASV, designated ML29, protects rodents and primates from LASV challenge and appears to be more attenuated than MOPV. To gain better insight into LASV-induced pathology and mechanism of attenuation we performed gene expression profiling in human peripheral blood mononuclear cells (PBMC) exposed to LASV and the vaccine candidate ML29. PBMC from healthy human subjects were exposed to either LASV or ML29. Although most PBMC are non-permissive for virus replication, they remain susceptible to signal transduction by virus particles. Total RNA was extracted and global gene expression was evaluated during the first 24 hours using high-density microarrays. Results were validated using RT-PCR, flow cytometry and ELISA. LASV and ML29 elicited differential expression of interferon-stimulated genes (ISG), as well as genes involved in apoptosis, NF-kB signaling and the coagulation pathways. These genes could eventually serve as biomarkers to predict disease outcomes. The remarkable differential expression of thrombomodulin, a key regulator of inflammation and coagulation, suggests its involvement with vascular abnormalities and mortality in Lassa fever disease.
has issue date	2013-09-12(xsd:dateTime)
bibo:doi	10.1371/journal.pntd.0002406
bibo:pmid	24069471
has license	cc0
sha1sum (hex)	0efede5fe4185af68b00ac20b99399af133e80bb
schema:url	https://doi.org/10.1371/journal.pntd.0002406
resource representing a document's title	Transcriptome Analysis of Human Peripheral Blood Mononuclear Cells Exposed to Lassa Virus and to the Attenuated Mopeia/Lassa Reassortant 29 (ML29), a Vaccine Candidate
has PubMed Central identifier	PMC3772037
has PubMed identifier	24069471
schema:publication	PLoS Negl Trop Dis
resource representing a document's body	covid:0efede5fe4185af68b00ac20b99399af133e80bb#body_text
is schema:about of	named entity 'susceptible' named entity 'African' named entity 'gene' named entity 'MONONUCLEAR' named entity 'ARENAVIRUSES' named entity 'HUMAN' named entity 'USING' named entity 'FLOW CYTOMETRY' named entity 'VASCULAR ABNORMALITIES' named entity 'LIVE' named entity 'PATHWAYS' named entity 'REGULATOR' named entity 'INDUCED' named entity 'ATTENUATED' named entity 'ELICITED' named entity 'LASSA VIRUS' named entity 'EVALUATED' named entity 'HUMAN SUBJECTS' named entity 'MECHANISM' named entity 'PRIMATES' named entity 'BIOMARKERS' named entity 'GENE EXPRESSION' named entity 'KEY' named entity 'EITHER' named entity 'MICROARRAYS' named entity 'RODENT' named entity 'PBMC' named entity 'COAGULATION' named entity 'INVOLVEMENT' named entity '24 HOURS' named entity 'ITS' named entity 'GLOBAL' named entity 'NON-' named entity 'RESULTS' named entity 'PERFORMED' named entity 'GENES' named entity 'DESIGNATED' named entity 'BETTER' named entity 'EXPOSED TO' named entity 'DIFFERENTIAL EXPRESSION' named entity 'SIGNAL TRANSDUCTION' named entity 'PREDICT' named entity 'TOTAL RNA' named entity 'SERVE' named entity 'PERIPHERAL BLOOD' named entity 'GAIN' named entity 'RODENTS' named entity 'HEALTHY' named entity 'RT-PCR' named entity 'PERIPHERAL BLOOD MONONUCLEAR CELLS' named entity 'STIMULATED' named entity 'MOPEIA VIRUS' named entity 'INTERFERON' named entity 'OUTCOMES' named entity 'TRANSCRIPTOME ANALYSIS' named entity 'ATTENUATION' named entity 'CAUSATIVE AGENT' named entity 'TO SIGNAL' named entity 'PATHOGENIC' named entity 'ELISA' named entity 'AFRICAN' named entity 'WEST AFRICA' named entity 'ANNUALLY' named entity 'DEATHS' named entity 'VALIDATED' named entity 'THESE' named entity 'APOPTOSIS' named entity 'GENE EXPRESSION PROFILING' named entity 'HUMAN' named entity 'EXTRACTED' named entity 'NF-KB' named entity 'SUSCEPTIBLE' named entity 'ML29' named entity 'BORNE' named entity 'PATHOLOGY' named entity 'DISEASE' named entity 'INSIGHT'

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