About: Rapid discovery and optimization of therapeutic antibodies against emerging infectious diseases

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About: Rapid discovery and optimization of therapeutic antibodies against emerging infectious diseases Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid discovery and optimization of therapeutic antibodies against emerging infectious diseases
Creator	Wong, K Li, J Keller, M Reed, D Tan, X Rogers, J Clements, T Dirmeier, R Frey, G Holland, T Kimmel, B Lewis, L Schoepp, R Schröder, O Tozer, E Woodnutt, G
Source	Medline; PMC
abstract	Using a comprehensive set of discovery and optimization tools, antibodies were produced with the ability to neutralize SARS coronavirus (SARS-CoV) infection in Vero E6 cells and in animal models. These anti-SARS antibodies were discovered using a novel DNA display method, which can identify new antibodies within days. Once neutralizing antibodies were identified, a comprehensive and effective means of converting the mouse sequences to human frameworks was accomplished using HuFR™ (human framework reassembly) technology. The best variant (61G4) from this screen showed a 3.5–4-fold improvement in neutralization of SARS-CoV infection in vitro. Finally, using a complete site-saturation mutagenesis methodology focused on the CDR (complementarity determining regions), a single point mutation (51E7) was identified that improved the 80% plaque reduction neutralization of the virus by greater than 8-fold. These discovery and evolution strategies can be applied to any emerging pathogen or toxin where a causative agent is known.
has issue date	2008-05-13(xsd:dateTime)
bibo:doi	10.1093/protein/gzn027
bibo:pmid	18480090
has license	cc-by-nc
sha1sum (hex)	0f2e41a966ec7d622762dc64ba63009e00a69bd5
schema:url	https://doi.org/10.1093/protein/gzn027
resource representing a document's title	Rapid discovery and optimization of therapeutic antibodies against emerging infectious diseases
has PubMed Central identifier	PMC2461042
has PubMed identifier	18480090
schema:publication	Protein Eng Des Sel
resource representing a document's body	covid:0f2e41a966ec7d622762dc64ba63009e00a69bd5#body_text
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