About: Identification and characterization of a virus-specific continuous B-cell epitope on the PrM/M protein of Japanese Encephalitis Virus: potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile Virus and Dengue Virus infections

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About: Identification and characterization of a virus-specific continuous B-cell epitope on the PrM/M protein of Japanese Encephalitis Virus: potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile Virus and Dengue Virus infections Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification and characterization of a virus-specific continuous B-cell epitope on the PrM/M protein of Japanese Encephalitis Virus: potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile Virus and Dengue Virus infections
Creator	Bu, Zhi-Gao Chen, Na-Sha Chen, Wei-Ye Deng, Yong-Qiang Ge, Jin-Ying Hu, Sen Hua, Rong-Hong Liu, Wen-Xin Qiao, Zu-Jian Qin, Cheng-Feng Wang, Xi-Jun Wen, Zhi-Yuan
Source	Medline; PMC
abstract	BACKGROUND: Differential diagnose of Japanese encephalitis virus (JEV) infection from other flavivirus especially West Nile virus (WNV) and Dengue virus (DV) infection was greatly hindered for the serological cross-reactive. Virus specific epitopes could benefit for developing JEV specific antibodies detection methods. To identify the JEV specific epitopes, we fully mapped and characterized the continuous B-cell epitope of the PrM/M protein of JEV. RESULTS: To map the epitopes on the PrM/M protein, we designed a set of 20 partially overlapping fragments spanning the whole PrM, fused them with GST, and expressed them in an expression vector. Linear epitope M14 ((105)VNKKEAWLDSTKATRY(120)) was detected by enzyme-linked immunosorbent assay (ELISA). By removing amino acid residues individually from the carboxy and amino terminal of peptide M14, we confirmed that the minimal unit of the linear epitope of PrM/M was M14-13 ((108)KEAWLDSTKAT(118)). This epitope was highly conserved across different JEV strains. Moreover, this epitope did not cross-react with WNV-positive and DENV-positive sera. CONCLUSION: Epitope M14-13 was a JEV specific lineal B-cell epitpe. The results may provide a useful basis for the development of epitope-based virus specific diagnostic clinical techniques.
has issue date	2010-09-22(xsd:dateTime)
bibo:doi	10.1186/1743-422x-7-249
bibo:pmid	20858291
has license	cc-by
sha1sum (hex)	13194f8e42df83d260e53ad9bf7448577e9319e5
schema:url	https://doi.org/10.1186/1743-422x-7-249
resource representing a document's title	Identification and characterization of a virus-specific continuous B-cell epitope on the PrM/M protein of Japanese Encephalitis Virus: potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile Virus and Dengue Virus infections
has PubMed Central identifier	PMC2954857
has PubMed identifier	20858291
schema:publication	Virol J
resource representing a document's body	covid:13194f8e42df83d260e53ad9bf7448577e9319e5#body_text
is schema:about of	named entity 'Virus' named entity 'cross-reactive' named entity 'partially' named entity 'JEV' named entity 'continuous' named entity 'Differential' named entity 'B-cell' named entity 'JEV' named entity 'highly' named entity 'Virus' named entity 'Japanese Encephalitis Virus' covid:arg/13194f8e42df83d260e53ad9bf7448577e9319e5 named entity 'specific' named entity 'fused' named entity 'Conclusion' named entity 'epitope' named entity 'PrM' named entity 'development' named entity 'epitopes' named entity 'West Nile virus' named entity 'epitope' named entity 'virus' named entity 'linear epitope' named entity 'JEV' named entity 'Linear epitope' named entity 'epitope' named entity 'enzyme-linked immunosorbent assay' named entity 'Virus' named entity 'Virus' named entity 'West Nile Virus' named entity 'B-cell' named entity 'M14' named entity 'MAb' named entity '450 nm' named entity 'MAb' named entity 'Western blot' named entity 'JEV' named entity 'skim milk' named entity 'Non-specific' named entity 'epitope' named entity 'peptides' named entity 'MAb' named entity 'fusion protein' named entity 'swine' named entity 'Vladivostok' named entity 'cell lysates' named entity 'JEV' named entity 'Virus' named entity 'Europe district' named entity 'protein' named entity 'GenBank' named entity 'biotechnology' named entity 'Beijing' named entity 'peptide' named entity 'secondary antibody' named entity 'peptide' named entity 'M14' named entity 'DENV' named entity 'Russia' named entity 'Tris-HCl' named entity 'GenBank' named entity 'M14' named entity 'WNV' named entity 'clones' named entity 'peptide' named entity 'Tween 20' named entity 'Flaviviridae' named entity 'highly conserved' named entity 'flaviviruses' named entity 'goat'

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