About: Resequencing microarray for detection of human adenoviruses in patients with conjunctivitis

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About: Resequencing microarray for detection of human adenoviruses in patients with conjunctivitis Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Resequencing microarray for detection of human adenoviruses in patients with conjunctivitis
Creator	Yuen, Kwok-Yung Miao, Ji Que, Tak-Lun Choi, Garnet Lau, Susanna Woo, Patrick Chan, Benedict Ellis-Behnke, Rutledge Fung, Hin-Tat Kam, Chak-Wah Ng, Kenneth Ngan, Antonio Shek, Kam-Chuen
source	Elsevier; Medline; PMC
abstract	BACKGROUND: Although high-density resequencing microarray is useful for detection and tracking the evolution of viruses associated with respiratory tract infections, no report on using this technology for the detection of viruses in patients with conjunctivitis is available. OBJECTIVES: To test if high-density resequencing microarray can be applied to detection of viruses in conjunctival swabs for patients with conjunctivitis. STUDY DESIGN: In this prospective proof-of-concept study, every 4 or 5 bacterial culture-negative conjunctival swab samples were pooled and subject to viral detection using TessArray™ Resequencing Pathogen Microarrays-Flu 3.1 (RPM-Flu-3.1). Results were compared with human adenovirus (HAdV) hexon gene PCR sequencing and viral culture. RESULTS: Thirty-two of the 38 conjunctival swab samples were bacterial culture-negative. Four of the 7 pooled samples were positive for HAdV using RPM-Flu-3.1. Hexon gene PCR sequencing on the 38 original individual samples showed that 3 and 4 samples contained HAdVs species D and B respectively. All the 6 samples that were positive for hexon gene PCR but negative for bacterial culture were also positive by the resequencing microarray. Viral culture was positive for HAdV type 3 in 1 sample, which was also positive by PCR and resequencing microarray. CONCLUSIONS: Resequencing microarray is as sensitive as PCR for detection of HAdV in conjunctival swabs. Unlike viral culture and hexon gene PCR sequencing, resequencing microarray was not able to differentiate the type and species of HAdV. Development of microarrays for conjunctivitis can be performed for rapid diagnosis of the viral cause of conjunctivitis.
has issue date	2010-01-13(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2009.12.014
bibo:pmid	20071220
has license	no-cc
sha1sum (hex)	1417e20893d38c3c99c96286bce5f4a12470bd33
schema:url	https://doi.org/10.1016/j.jcv.2009.12.014
resource representing a document's title	Resequencing microarray for detection of human adenoviruses in patients with conjunctivitis
has PubMed Central identifier	PMC7172538
has PubMed identifier	20071220
schema:publication	J Clin Virol
resource representing a document's body	covid:1417e20893d38c3c99c96286bce5f4a12470bd33#body_text
is schema:about of	named entity 'conjunctivitis' named entity 'respiratory tract infections' named entity 'viral' named entity 'swabs' named entity 'gene' named entity 'swabs' named entity 'PCR' named entity 'viruses' named entity 'OBJECTIVES' named entity 'RESULTS' named entity 'GENE' named entity 'POOLED' named entity 'TYPE' named entity 'EVOLUTION' named entity 'SENSITIVE' named entity 'PROSPECTIVE' named entity 'OF-' named entity 'RESPIRATORY TRACT INFECTIONS' named entity 'PCR' named entity 'proof-of-concept' named entity 'Pathogen' named entity 'samples' named entity 'bacterial' named entity 'tracking' named entity 'conjunctivitis' named entity 'conjunctivitis' named entity 'microarray analysis' named entity 'Flu' named entity 'Vero cells' named entity 'Hexon' named entity 'hexon' named entity 'conjunctival' named entity 'Shaw Foundation' named entity 'hexon' named entity 'gene' named entity 'microarray' named entity 'viruses' named entity 'viruses' named entity 'gene' named entity 'microarray' named entity 'Flu' named entity 'morbidity' named entity 'gene' named entity 'Accident and Emergency Department' named entity 'viruses' named entity 'virus' named entity 'A24' named entity 'Viral culture' named entity 'gene sequencing' named entity 'viral culture' named entity 'conjunctivitis' named entity 'proof-of-concept' named entity 'viruses' named entity 'viruses' named entity 'PCR' named entity 'gene sequences' named entity 'viruses' named entity 'HKU1' named entity '12:7' named entity 'parainfluenza' named entity 'Flu' named entity 'gene' named entity 'microarray' named entity 'conjunctivitis' named entity 'PCR' named entity 'antigen' named entity 'conjunctivitis' named entity 'viral' named entity 'PCR'

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