About: Lack of a 5.9 kDa Peptide C-Terminal Fragment of Fibrinogen α Chain Precedes Fibrosis Progression in Patients with Liver Disease

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About: Lack of a 5.9 kDa Peptide C-Terminal Fragment of Fibrinogen α Chain Precedes Fibrosis Progression in Patients with Liver Disease Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Lack of a 5.9 kDa Peptide C-Terminal Fragment of Fibrinogen α Chain Precedes Fibrosis Progression in Patients with Liver Disease
Creator	Casals, Gregori Crespo, Gonzalo Forns, Xavier Marfà, Santiago Morales-Ruiz, Manuel Navasa, Miquel Reichenbach, Vedrana Jimé Nez, Wladimiro
Source	PMC
abstract	Early detection of fibrosis progression is of major relevance for the diagnosis and management of patients with liver disease. This study was designed to find non-invasive biomarkers for fibrosis in a clinical context where this process occurs rapidly, HCV-positive patients who underwent liver transplantation (LT). We analyzed 93 LT patients with HCV recurrence, 41 non-LT patients with liver disease showing a fibrosis stage F≥1 and 9 patients without HCV recurrence who received antiviral treatment before LT, as control group. Blood obtained from 16 healthy subjects was also analyzed. Serum samples were fractionated by ion exchange chromatography and their proteomic profile was analyzed by SELDI-TOF-MS. Characterization of the peptide of interest was performed by ion chromatography and electrophoresis, followed by tandem mass spectrometry identification. Marked differences were observed between the serum proteome profile of LT patients with early fibrosis recurrence and non-recurrent LT patients. A robust peak intensity located at 5905 m/z was the distinguishing feature of non-recurrent LT patients. However, the same peak was barely detected in recurrent LT patients. Similar results were found when comparing samples of healthy subjects with those of non-LT fibrotic patients, indicating that our findings were not related to either LT or HCV infection. Using tandem mass-spectrometry, we identified the protein peak as a C-terminal fragment of the fibrinogen α chain. Cell culture experiments demonstrated that TGF-β reduces α-fibrinogen mRNA expression and 5905 m/z peak intensity in HepG2 cells, suggesting that TGF-β activity regulates the circulating levels of this protein fragment. In conclusion, we identified a 5.9 kDa C-terminal fragment of the fibrinogen α chain as an early serum biomarker of fibrogenic processes in patients with liver disease.
has issue date	2014-10-02(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0109254
bibo:pmid	25275549
has license	cc-by
sha1sum (hex)	1542e8e707bd5fa1cb6b3919722fc30ced018c1f
schema:url	https://doi.org/10.1371/journal.pone.0109254
resource representing a document's title	Lack of a 5.9 kDa Peptide C-Terminal Fragment of Fibrinogen α Chain Precedes Fibrosis Progression in Patients with Liver Disease
has PubMed Central identifier	PMC4183580
has PubMed identifier	25275549
schema:publication	PLoS One
resource representing a document's body	covid:1542e8e707bd5fa1cb6b3919722fc30ced018c1f#body_text
is schema:about of	named entity 'LIVER DISEASE' named entity 'fibrosis' named entity 'peak' named entity 'samples' named entity 'peptide' named entity 'Fibrosis' named entity 'C-TERMINAL' named entity 'NON-' named entity 'CONCLUSION' named entity 'EXPERIMENTS' named entity 'TANDEM MASS SPECTROMETRY' named entity 'SIMILAR' named entity 'MRNA EXPRESSION' named entity 'IDENTIFIED' named entity 'NON-INVASIVE' named entity 'PROGRESSION' named entity 'LIVER DISEASE' named entity 'LIVER TRANSPLANTATION' named entity 'RELATED' named entity 'RAPIDLY' named entity 'SERUM' named entity 'HCV' named entity 'HEALTHY SUBJECTS' named entity 'EITHER' named entity 'ION EXCHANGE CHROMATOGRAPHY' named entity 'OF INTEREST' named entity 'EARLY DETECTION' named entity 'RELEVANCE' named entity 'PROCESS' named entity 'ANALYZED' named entity 'RESULTS' named entity 'PROTEOMIC PROFILE' named entity 'CHARACTERIZATION' named entity 'MANAGEMENT' named entity 'OUR' named entity 'IDENTIFICATION' named entity 'MS.' named entity 'COMPARING' named entity 'FOUND' named entity 'PEAK INTENSITY' named entity 'TGF-B' named entity 'CELL CULTURE' named entity 'FIBROTIC' named entity 'SPECTROMETRY' named entity 'MAJOR' named entity 'KDA' named entity 'PEPTIDE' named entity 'SAMPLES' named entity 'PROTEIN ' named entity 'FINDINGS' named entity 'BLOOD' named entity 'ROBUST' named entity 'FRAGMENT OF' named entity 'CHAIN' named entity 'POSITIVE' named entity 'ION CHROMATOGRAPHY ' named entity 'MARKED' named entity 'CELLS' named entity 'USING' named entity 'INDICATING' named entity 'HCV INFECTION' named entity 'PROTEOME PROFILE' named entity 'WHERE' named entity 'FIBROSIS' named entity 'FRACTIONATED' named entity 'PERFORMED BY' named entity 'FOLLOWED BY' named entity 'FEATURE' named entity 'MASS' named entity 'NON-RECURRENT' named entity 'FIBROSIS' named entity 'KDA' named entity 'FIBRINOGEN' named entity 'CHAIN'

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