About: Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells
Creator	Herrler, Georg Ren, Xiaofeng Enjuanes, Luis Cong, Yingying Zhou, Xingdong Li, Guangxing Meng, Fandan Bai, Yunyun Cong, Chengcheng Li, Xiaoxue Lv, Xiaonan Qu, Bo
source	Elsevier; Medline; PMC
abstract	Abstract Infection of polarized intestinal epithelial cells by porcine epidemic diarrhea virus (PEDV) was characterized. Indirect immunofluorescence assay, real-time PCR, and transmission electron microscopy confirmed PEDV can be successfully propagated in immortalized swine small intestine epithelial cells (IECs). Infection involved porcine aminpeptidase N (pAPN), a reported cellular receptor for PEDV, transient expression of pAPN and siRNA targeted pAPN increased and decreased the infectivity of PEDV in IECs, respectively. Subsequently, polarized entry into and release from both Vero E6 and IECs was analyzed. PEDV entry into polarized cells and pAPN grown on membrane inserts occurs via apical membrane. The progeny virus released into the medium was also quantified which demonstrated that PEDV is preferentially released from the apical membrane. Collectively, our data demonstrate that pAPN, the cellular receptor for PEDV, mediates polarized PEDV infection. These results imply the possibility that PEDV infection may proceed by lateral spread of virus in intestinal epithelial cells.
has issue date	2015-04-30(xsd:dateTime)
bibo:doi	10.1016/j.virol.2015.01.020
bibo:pmid	25681796
has license	els-covid
sha1sum (hex)	1a6aea516a8e2d848685704f39a0a00b74eef125
schema:url	https://doi.org/10.1016/j.virol.2015.01.020
resource representing a document's title	Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells
has PubMed Central identifier	PMC7112081
has PubMed identifier	25681796
schema:publication	Virology
resource representing a document's body	covid:1a6aea516a8e2d848685704f39a0a00b74eef125#body_text
is schema:about of	named entity 'mediates' named entity 'immortalized' named entity 'mediated' named entity 'PORCINE' named entity 'SPREAD' named entity 'EPITHELIAL CELLS' named entity 'INSERTS' named entity 'CHARACTERIZED' named entity 'DECREASED' named entity 'CELLULAR' named entity 'INFECTIVITY' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'OUR' named entity 'ENTRY' named entity 'DEMONSTRATED' named entity 'MEDIUM' named entity 'SIRNA' named entity 'INFECTION' named entity 'INFECTION' named entity 'IMMUNOFLUORESCENCE ASSAY' named entity 'RELEASED' named entity 'REPORTED' named entity 'THESE' named entity 'INTESTINAL' named entity 'DATA' named entity 'CELLS' named entity 'VIRUS' named entity 'INDIRECT IMMUNOFLUORESCENCE' named entity 'RECEPTOR' named entity 'VERO' named entity 'LATERAL' named entity 'TARGETED' named entity 'PORCINE' named entity 'MEMBRANE' named entity 'MEDIATED' named entity 'AMINOPEPTIDASE N' named entity 'TARGET CELLS' named entity 'APICAL MEMBRANE' named entity 'TRANSIENT EXPRESSION' named entity 'RESULTS' named entity 'POSSIBILITY' named entity 'SMALL INTESTINE' named entity 'RELEASE' named entity 'TRANSMISSION ELECTRON MICROSCOPY' named entity 'REAL-TIME PCR' named entity 'IMMORTALIZED' named entity 'SWINE' named entity 'INVOLVED' named entity 'ANALYZED' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'INCREASED' named entity 'CONFIRMED' named entity 'PROGENY' named entity 'MEDIATES' named entity 'lateral' named entity 'infectivity' named entity 'virus' named entity 'polarized' named entity 'Vero' named entity 'cellular receptor' named entity 'apical membrane' named entity 'lateral' named entity 'small intestine' named entity 'apical membrane' named entity 'PEDV' named entity 'virus' named entity 'transmission electron microscopy' named entity 'PEDV' named entity 'immunofluorescence assay' named entity 'virus'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software