About: Expression of HIV-1 antigens in plants as potential subunit vaccines

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About: Expression of HIV-1 antigens in plants as potential subunit vaccines Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Expression of HIV-1 antigens in plants as potential subunit vaccines
Creator	Chakauya, Ereck Lynch, Alisson Maclean, James Meyers, Ann Shephard, Enid Williamson, Anna-Lise Rybicki, Edward Tanzer, Fiona
Source	Medline; PMC
abstract	BACKGROUND: Human immunodeficiency virus type 1 (HIV-1) has infected more than 40 million people worldwide, mainly in sub-Saharan Africa. The high prevalence of HIV-1 subtype C in southern Africa necessitates the development of cheap, effective vaccines. One means of production is the use of plants, for which a number of different techniques have been successfully developed. HIV-1 Pr55Gag is a promising HIV-1 vaccine candidate: we compared the expression of this and a truncated Gag (p17/p24) and the p24 capsid subunit in Nicotiana spp. using transgenic plants and transient expression via Agrobacterium tumefaciens and recombinant tobamovirus vectors. We also investigated the influence of subcellular localisation of recombinant protein to the chloroplast and the endoplasmic reticulum (ER) on protein yield. We partially purified a selected vaccine candidate and tested its stimulation of a humoral and cellular immune response in mice. RESULTS: Both transient and transgenic expression of the HIV antigens were successful, although expression of Pr55Gag was low in all systems; however, the Agrobacterium-mediated transient expression of p24 and p17/p24 yielded best, to more than 1 mg p24/kg fresh weight. Chloroplast targeted protein levels were highest in transient and transgenic expression of p24 and p17/p24. The transiently-expressed p17/p24 was not immunogenic in mice as a homologous vaccine, but it significantly boosted a humoral and T cell immune response primed by a gag DNA vaccine, pTHGagC. CONCLUSION: Transient agroinfiltration was best for expression of all of the recombinant proteins tested, and p24 and p17/p24 were expressed at much higher levels than Pr55Gag. Our results highlight the usefulness of plastid signal peptides in enhancing the production of recombinant proteins meant for use as vaccines. The p17/p24 protein effectively boosted T cell and humoral responses in mice primed by the DNA vaccine pTHGagC, showing that this plant-produced protein has potential for use as a vaccine.
has issue date	2008-06-23(xsd:dateTime)
bibo:doi	10.1186/1472-6750-8-53
bibo:pmid	18573204
has license	cc-by
sha1sum (hex)	1b7c700ee3a33c0a675a5f7e71a20ce62467b900
schema:url	https://doi.org/10.1186/1472-6750-8-53
resource representing a document's title	Expression of HIV-1 antigens in plants as potential subunit vaccines
has PubMed Central identifier	PMC2443125
has PubMed identifier	18573204
schema:publication	BMC Biotechnol
resource representing a document's body	covid:1b7c700ee3a33c0a675a5f7e71a20ce62467b900#body_text
is schema:about of	named entity 'tobamovirus' named entity 'prevalence' named entity 'protein' named entity 'transgenic plants' named entity 'MORE THAN' named entity 'EFFECTIVE' named entity 'MEANS' named entity 'PRODUCTION' named entity 'CELLULAR IMMUNE RESPONSE' named entity 'VECTORS' named entity 'ENDOPLASMIC RETICULUM' named entity 'INVESTIGATED' named entity 'WORLDWIDE' named entity 'HUMAN IMMUNODEFICIENCY VIRUS TYPE 1' named entity 'IS A' named entity 'EXPRESSION' named entity 'PARTIALLY PURIFIED' named entity 'TRANSGENIC PLANTS' named entity 'VACCINE CANDIDATE' named entity 'AGROBACTERIUM TUMEFACIENS' named entity 'PEOPLE' named entity 'COMPARED' named entity 'HAVE' named entity 'PLANTS' named entity 'HIV-1' named entity 'VACCINES' named entity 'SPP' named entity 'INFECTED' named entity 'USE OF' named entity 'MICE' named entity 'NICOTIANA' named entity 'SELECTED' named entity 'CAPSID' named entity 'SUB-SAHARAN AFRICA' named entity 'EXPRESSION' named entity 'SUBTYPE' named entity 'P24' named entity 'TOBAMOVIRUS' named entity 'TECHNIQUES' named entity 'STIMULATION' named entity 'ANTIGENS' named entity 'SUBUNIT VACCINES' named entity 'ITS' named entity 'PLANTS' named entity 'POTENTIAL' named entity 'YIELD' named entity 'CHLOROPLAST' named entity 'HIGH PREVALENCE' named entity 'SOUTHERN AFRICA' named entity 'GAG' named entity 'TRUNCATED' named entity 'RECOMBINANT PROTEIN' named entity 'PROTEIN ' named entity 'INFLUENCE' named entity 'BACKGROUND' named entity 'USING' named entity 'NUMBER OF' named entity 'RECOMBINANT' named entity 'MILLION' named entity 'TESTED' named entity 'LOCALISATION' named entity 'HIV-1' named entity 'DIFFERENT' named entity 'TRANSIENT EXPRESSION' named entity 'SUBUNIT' named entity 'DEVELOPMENT' covid:arg/1b7c700ee3a33c0a675a5f7e71a20ce62467b900 named entity 'production' named entity 'Agrobacterium tumefaciens' named entity 'purified' named entity 'HIV-1' named entity 'cellular immune response' named entity 'transgenic plants' named entity 'p17'

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