About: Large-scale purification and crystallization of the endoribonuclease XendoU: troubleshooting with His-tagged proteins

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About: Large-scale purification and crystallization of the endoribonuclease XendoU: troubleshooting with His-tagged proteins Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Large-scale purification and crystallization of the endoribonuclease XendoU: troubleshooting with His-tagged proteins
Creator	Arceci, Massimo Bozzoni, Irene Caffarelli, Elisa Laneve, Pietro Brunori, Maurizio Renzi, Fabiana Vallone, Beatrice Bolognetti, Cenci Panetta, Gianna Pasteur-Fondazione, Istituto
source	Medline; PMC
abstract	XendoU is the first endoribonuclease described in higher eukaryotes as being involved in the endonucleolytic processing of intron-encoded small nucleolar RNAs. It is conserved among eukaryotes and its viral homologue is essential in SARS replication and transcription. The large-scale purification and crystallization of recombinant XendoU are reported. The tendency of the recombinant enzyme to aggregate could be reversed upon the addition of chelating agents (EDTA, imidazole): aggregation is a potential drawback when purifying and crystallizing His-tagged proteins, which are widely used, especially in high-throughput structural studies. Purified monodisperse XendoU crystallized in two different space groups: trigonal P3(1)21, diffracting to low resolution, and monoclinic C2, diffracting to higher resolution.
has issue date	2006-02-28(xsd:dateTime)
bibo:doi	10.1107/s1744309106006373
bibo:pmid	16511328
has license	green-oa
sha1sum (hex)	1f10dab2bf87ba6ea015acdaa43bab0028f7dabc
schema:url	https://doi.org/10.1107/s1744309106006373
resource representing a document's title	Large-scale purification and crystallization of the endoribonuclease XendoU: troubleshooting with His-tagged proteins
has PubMed Central identifier	PMC2197201
has PubMed identifier	16511328
schema:publication	Acta Crystallographica Section F Structural Biology and Crystallization Communications
resource representing a document's body	covid:1f10dab2bf87ba6ea015acdaa43bab0028f7dabc#body_text
is schema:about of	named entity 'crystallization' named entity 'eukaryotes' named entity 'proteins' named entity 'crystallization' named entity 'STUDIES' named entity 'INVOLVED' named entity 'SARS' named entity 'AGGREGATION' named entity 'SMALL NUCLEOLAR RNAS' named entity 'REPLICATION' named entity 'INTRON' named entity 'CRYSTALLIZATION' named entity 'HOMOLOGUE' named entity 'TRANSCRIPTION' named entity 'VIRAL' named entity 'MONOCLINIC' named entity 'REVERSED' named entity 'PURIFYING' named entity 'PROTEINS' named entity 'HIGHER' named entity 'DIFFERENT' named entity 'GROUPS' named entity 'TAGGED' named entity 'HIS-' named entity 'POTENTIAL' covid:arg/1f10dab2bf87ba6ea015acdaa43bab0028f7dabc named entity 'SCALE' named entity 'HIS-' named entity 'LARGE' named entity 'STRUCTURAL BIOLOGY' named entity 'PURIFICATION' named entity 'COMMUNICATIONS' named entity 'ENDORIBONUCLEASE' named entity 'TENDENCY' named entity 'AGGREGATE' named entity 'USED' named entity 'LARGE' named entity 'ENCODED' named entity 'EUKARYOTES' named entity 'IS A' named entity 'PURIFICATION' named entity 'ITS' named entity 'RECOMBINANT ENZYME' named entity 'LOW RESOLUTION' named entity 'STRUCTURAL' named entity 'SPACE' named entity 'CRYSTALLIZED' named entity 'COULD BE' named entity 'RESOLUTION' named entity 'RECOMBINANT' named entity 'REPORTED' named entity 'ESSENTIAL' named entity 'CONSERVED' named entity 'BEING' named entity 'PURIFIED' named entity 'IMIDAZOLE' named entity 'PROCESSING' named entity 'CHELATING AGENTS' named entity 'ADDITION' named entity 'DESCRIBED' named entity 'ENDORIBONUCLEASE' named entity 'PROTEINS' named entity 'TROUBLESHOOTING' named entity 'CRYSTALLIZATION' named entity 'SCALE' named entity 'TAGGED' named entity 'MONODISPERSE' named entity 'diffracting' named entity 'trigonal' named entity 'conserved' named entity 'essential' named entity 'space groups'

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