About: Isolation of a Leuconostoc mesenteroides Strain With Anti-Porcine Epidemic Diarrhea Virus Activities From Kefir Grains

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About: Isolation of a Leuconostoc mesenteroides Strain With Anti-Porcine Epidemic Diarrhea Virus Activities From Kefir Grains Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Isolation of a Leuconostoc mesenteroides Strain With Anti-Porcine Epidemic Diarrhea Virus Activities From Kefir Grains
Creator	Chang, Hui-Wen Chang-Liao, Wan-Ping Chiu, Yu-Han Dufossé, Laurent Kos, Blaženka Lee, An Liu, Je-Ruei Veljovic, Katarina
source	Medline; PMC
abstract	Swine grown under commercial conditions are vulnerable to environmental exposure to several viruses, which may cause infectious diseases and spread easily and rapidly, resulting in significant economic losses in animal husbandry. Previous studies have suggested that probiotics seem to be a new and promising alternative to vaccinations to protect animals against potential viral infections. In this study, we used the Vero cell culture model of infection to study porcine epidemic diarrhea virus (PEDV). We screened lactic acid bacteria (LAB) with anti-PEDV potential from kefir grains, which are starter cultures used to ferment milk into kefir. Twenty-nine LAB strains were isolated and identified as Enterococcus durans, Lactobacillus kefiri, Lactococcus lactis, and Leuconostoc mesenteroides, according to 16S ribosomal RNA (rRNA) and rpoA gene sequence analyses. The anti-PEDV activities of the LAB intracellular extracts were compared, and the intracellular extracts of Ln. mesenteroides showed higher anti-PEDV activities than that of the other species. Among the Ln. mesenteroides strains, a strain designated YPK30 showed a higher growth rate than that of the other strains and was further evaluated for its anti-PEDV activity. The results showed that the intracellular extracts of Ln. mesenteroides YPK30 possessed in vitro prophylactic, therapeutic, and direct-inhibitory effects against PEDV in the Vero cell model. The expression levels of Type 1 interferon (IFN)-dependent genes, including Myxovirus resistance 1 (MX1) and interferon-stimulated gene 15 (ISG15), were significantly increased after treatment with intracellular extracts of Ln. mesenteroides YPK30 for 24 h. Such expression suggests that the anti-PEDV activity of Ln. mesenteroides YPK30 could be attributed to its up-regulatory effect on the expression of MX1 and ISG15 genes. These results suggested that Ln. mesenteroides YPK30 has the potential to provide some levels of host protection against PEDV infections.
has issue date	2020-07-15(xsd:dateTime)
bibo:doi	10.3389/fmicb.2020.01578
bibo:pmid	32760370
has license	cc-by
sha1sum (hex)	1f2e5eb6de71771f38f27b75b91e56310f7ae4c9
schema:url	https://doi.org/10.3389/fmicb.2020.01578
resource representing a document's title	Isolation of a Leuconostoc mesenteroides Strain With Anti-Porcine Epidemic Diarrhea Virus Activities From Kefir Grains
has PubMed Central identifier	PMC7373756
has PubMed identifier	32760370
schema:publication	Front Microbiol
resource representing a document's body	covid:1f2e5eb6de71771f38f27b75b91e56310f7ae4c9#body_text
is schema:about of	named entity 'EXTRACTS' named entity 'GENE' named entity 'ENTEROCOCCUS DURANS' named entity 'CAUSE' named entity 'FERMENT' named entity 'ISOLATED' named entity 'VERO CELL' named entity 'INCREASED' named entity 'HAVE' named entity 'ANIMAL HUSBANDRY' named entity 'SPECIES' named entity 'VIRUSES' named entity 'DMSO' named entity 'nonmotile' named entity '16S rRNA' named entity 'Ln. mesenteroides' named entity 'PBS' named entity 'Lactococcus' named entity 'LAB' named entity 'vaccines' named entity 'IBM' named entity 'IFN-β' named entity 'porcine parvovirus' named entity 'IFN' named entity 'New York' named entity 'ISG15' named entity 'incubation' named entity 'dairy products' named entity 'viral titers' named entity 'IFN' named entity 'Vero cells' named entity 'viruses' named entity 'positive control' named entity 'Lactobacillus' named entity 'Vero cells' named entity 'type 2' named entity 'ubiquitin' named entity 'Gram stain' named entity 'PEDV' named entity 'probiotics' named entity 'kefir grains' named entity 'intracellular' named entity 'immunomodulatory' named entity 'PEDV' named entity 'porcine circovirus' named entity 'Intracellular' named entity 'cell line' named entity 'MX1' named entity 'virus' named entity 'OAS1' named entity 'intracellular' named entity 'cell lines' named entity 'α-methyl-D-glucoside' named entity '37°C' named entity 'immunomodulatory' named entity 'Sigma-Aldrich' named entity 'standard deviations' named entity 'metabolic activity' named entity 'Vero cells' named entity 'Alphacoronavirus' named entity 'S-layer' named entity 'formazan' named entity 'Vero cells' named entity 'kimchi' named entity 'Ln. mesenteroides' named entity 'Kluyveromyces' named entity 'nucleocapsids' named entity 'N-acetyl glucosamine' named entity 'microscopic observations' named entity 'RNA polymerase' named entity 'Microscopic observation' named entity 'PBS' named entity 'ethanol' named entity 'microorganisms'

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