About: The impact of protein characterization in structural proteomics

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About: The impact of protein characterization in structural proteomics Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The impact of protein characterization in structural proteomics
Creator	Brown, J Gilbert, R Coutard, B Nordlund, P Owens, R Svergun, D Egloff, M.-P Enguita, F Fogg, M Geerlof, Arie Groves, M Haouz, A Nettleship, J Ruff, M Sainsbury, S Wilmanns, Matthias
Source	Medline; PMC
abstract	Protein characterization plays a role in two key aspects of structural proteomics. The first is the quality assessment of the produced protein preparations. Obtaining well diffracting crystals is one of the major bottlenecks in the structure‐determination pipeline. Often, this is caused by the poor quality of the protein preparation used for crystallization trials. Hence, it is essential to perform an extensive quality assessment of the protein preparations prior to crystallization and to use the results in the evaluation of the process. Here, a protein‐production and crystallization strategy is proposed with threshold values for protein purity (95%) and monodispersity (85%) below which a further optimization of the protein‐production process is strongly recommended. The second aspect is the determination of protein characteristics such as domains, oligomeric state, post‐translational modifications and protein–protein and protein–ligand interactions. In this paper, applications and new developments of protein‐characterization methods using MS, fluorescence spectroscopy, static light scattering, analytical ultracentrifugation and small‐angle X‐ray scattering within the EC Structural Proteomics in Europe contract are described. Examples of the application of the various methods are given.
has issue date	2006-10-04(xsd:dateTime)
bibo:doi	10.1107/s0907444906030307
bibo:pmid	17001090
has license	no-cc
sha1sum (hex)	220f9a2fbceef0d3c30b3f7a346946c3a125e183
schema:url	https://doi.org/10.1107/s0907444906030307
resource representing a document's title	The impact of protein characterization in structural proteomics
has PubMed Central identifier	PMC7161605
has PubMed identifier	17001090
schema:publication	Acta Crystallogr D Biol Crystallogr
resource representing a document's body	covid:220f9a2fbceef0d3c30b3f7a346946c3a125e183#body_text
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