About: Intermediates in the constitutive and regulated secretory pathways released in vitro from semi-intact cells

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Intermediates in the constitutive and regulated secretory pathways released in vitro from semi-intact cells
Creator	Kelly, Regis Grimes, Mark
source	PMC
abstract	Regulated secretory cells have two pathways that transport secreted proteins from the Golgi complex to the cell surface. To identify carrier vesicles involved in regulated and constitutive secretion, PC12 pheochromocytoma cells were labeled with [35S]sulfate to identify markers for the two secretory pathways, then mechanically permeabilized and incubated in vitro. Small constitutive secretory vesicles, containing mostly sulfated proteoglycans, accumulated during an in vitro incubation with ATP. In the presence of GTP gamma S, the constitutive vesicles became significantly more dense, suggesting that a coated intermediate was stabilized. Larger immature regulated secretory granules, enriched in sulfated secretogranin II, also escaped from the permeabilized cells in vitro. During granule maturation, their density increased and the amount of cofractionating proteoglycans diminished. The data suggest that sorting continues during secretory granule maturation.
has issue date	1992-05-01(xsd:dateTime)
bibo:pmid	1572894
has license	cc-by-nc-sa
sha1sum (hex)	23b89b2ee6a5870dd94c479b892cda6a54c8ab6f
resource representing a document's title	Intermediates in the constitutive and regulated secretory pathways released in vitro from semi-intact cells
has PubMed Central identifier	PMC2289447
has PubMed identifier	1572894
schema:publication	J Cell Biol
resource representing a document's body	covid:23b89b2ee6a5870dd94c479b892cda6a54c8ab6f#body_text
is schema:about of	named entity 'INTACT' named entity 'secretory pathways' named entity 'sulfated' named entity 'donor' named entity 'constitutive' named entity 'donor' named entity 'IN VITRO' named entity 'PATHWAYS' named entity 'PATHWAYS' named entity 'REGULATED' named entity 'LABELED' named entity 'SECRETORY' named entity 'DONOR' named entity 'DIFFERENT' named entity 'MARKERS' named entity 'PRESENCE OF' named entity 'PC12' named entity 'CONTAINING' named entity 'POST' named entity 'MIND' named entity 'PHEOCHROMOCYTOMA CELLS' named entity 'CONSIDERABLE' named entity 'TO EXPLAIN' named entity 'SECRETORY' named entity 'REGULATED' named entity 'RELEASED' named entity 'INTRA-GOLGI TRANSPORT' named entity 'CELLS' named entity 'THESE' named entity 'TO IDENTIFY' named entity 'GOLGI COMPLEX' named entity 'SECRETED' named entity 'INVOLVED' named entity 'COMPARTMENTS' named entity 'PROTEINS' named entity 'HOW' named entity 'SORTED' named entity 'ER TO GOLGI TRANSPORT' named entity 'TRANSPORT' named entity 'EFFORT' named entity 'CARRIER' named entity 'COMPARTMENT' named entity 'PROTEOGLYCANS' named entity 'FUSE' named entity 'INCUBATION' named entity 'GOLGI' named entity 'THE CELL' named entity 'VESICLE' named entity 'BUD' named entity 'IDENTIFYING' named entity 'INCUBATED' named entity 'INTERMEDIATES' named entity 'THE CARRIER' named entity 'ORGANELLES' named entity 'TRAFFIC' named entity 'SUBSET' named entity 'ATP' named entity 'ACCUMULATED' named entity 'NEED' named entity 'MEMBRANE COMPARTMENT' named entity 'ACCEPTOR' named entity 'SMALL' named entity 'MEMBRANE PROTEINS' named entity 'PRESENT' named entity 'IN VITRO' named entity 'TRANS' named entity 'SECRETION' named entity 'HAVE' named entity 'SECRETORY CELLS' named entity 'SECRETORY VESICLES' named entity 'CELL SURFACE' named entity 'VESICLES' named entity 'proteins' named entity 'pheochromocytoma' named entity 'subset' named entity 'Golgi' named entity 'transport' named entity 'accumulated' named entity 'surface' named entity 'vesicles' named entity 'Vitro' named entity 'sulfate'

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