About: In vitro inhibition of CSFV replication by multiple siRNA expression

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About: In vitro inhibition of CSFV replication by multiple siRNA expression Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	In vitro inhibition of CSFV replication by multiple siRNA expression
Creator	Guo, Huancheng Tu, Changchun Li, Jiangnan Liu, Shuai Dai, Yajuan Ouyang, Hongsheng Wang, Tiedong
Source	Elsevier; Medline; PMC
abstract	Abstract Classical swine fever (CSF) is a highly contagious viral disease of pigs which causes major economic losses worldwide. No specific drug is currently available for the effective treatment of CSFV infection; however, RNA interference (RNAi) has been applied successfully to inhibit the replication of human and other animal viruses. In this study, three effective siRNAs targeting NS3 of CSFV were selected. siNS3-2 targeting NS3 gene was chosen for further experimentation, while siN1 and siN2 targeting Npro gene, and siNS5B targeting NS5B gene describe previously. Single, double and quadruple anti-CSFV siRNA expression plasmids, with loxp sites at each end of the selectable marker genes, were constructed and analyzed using the same promoters or four different promoters, targeting Npro , NS3 and NS5B genes of CSFV. Results indicate that single or multiple siRNA expression plasmids can efficiently inhibit CSFV replication and that inhibition was markedly stronger when multiple siRNAs were expressed targeting different genes of CSFV. Since RNAi applied to anti-CSFV research, this study provides anti-CSFV methods by single and multiple siRNA expression which can target most viral isolates of different subtypes and prevent viral escape. It also provides a basis for development of CSFV-resistant transgenic pigs.
has issue date	2011-08-31(xsd:dateTime)
bibo:doi	10.1016/j.antiviral.2011.06.005
bibo:pmid	21699919
has license	els-covid
sha1sum (hex)	246b2f3f58779c6da0045b7bb0a9560fa3978131
schema:url	https://doi.org/10.1016/j.antiviral.2011.06.005
resource representing a document's title	In vitro inhibition of CSFV replication by multiple siRNA expression
has PubMed Central identifier	PMC7114328
has PubMed identifier	21699919
schema:publication	Antiviral Research
resource representing a document's body	covid:246b2f3f58779c6da0045b7bb0a9560fa3978131#body_text
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