About: Differential cell line susceptibility to the emerging Zika virus: implications for disease pathogenesis, non-vector-borne human transmission and animal reservoirs

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About: Differential cell line susceptibility to the emerging Zika virus: implications for disease pathogenesis, non-vector-borne human transmission and animal reservoirs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Differential cell line susceptibility to the emerging Zika virus: implications for disease pathogenesis, non-vector-borne human transmission and animal reservoirs
Creator	Sridhar, Siddharth Jin, Dong-Yan Yuen, Kwok-Yung Chan, Kwok-Hung Chan, Chris Chung-Sing Tee, Kah-Meng Kok, Kin-Hang Lu, Gang Zhu, Zheng Cai, Jian-Piao Tsao, Sai-Wah Chik-Yan Yip, Cyril Chan, Fuk-Woo, Jasper Oi, Jessica Tsang, Ling Zhang, Anna Chik, Kenn, Ka-Heng Chiu, Kin Cheuk, Amy Kwan-Yue Choi, Garnet Lo, Yin
source	Medline; PMC
abstract	Zika virus (ZIKV) is unique among human-pathogenic flaviviruses by its association with congenital anomalies and trans-placental and sexual human-to-human transmission. Although the pathogenesis of ZIKV-associated neurological complications has been reported in recent studies, key questions on the pathogenesis of the other clinical manifestations, non-vector-borne transmission and potential animal reservoirs of ZIKV remain unanswered. We systematically characterized the differential cell line susceptibility of 18 human and 15 nonhuman cell lines to two ZIKV isolates (human and primate) and dengue virus type 2 (DENV-2). Productive ZIKV replication (⩾2 log increase in viral load, ZIKV nonstructural protein-1 (NS1) protein expression and cytopathic effects (CPE)) was found in the placental (JEG-3), neuronal (SF268), muscle (RD), retinal (ARPE19), pulmonary (Hep-2 and HFL), colonic (Caco-2),and hepatic (Huh-7) cell lines. These findings helped to explain the trans-placental transmission and other clinical manifestations of ZIKV. Notably, the prostatic (LNCaP), testicular (833KE) and renal (HEK) cell lines showed increased ZIKV load and/or NS1 protein expression without inducing CPE, suggesting their potential roles in sexual transmission with persistent viral replication at these anatomical sites. Comparatively, none of the placental and genital tract cell lines allowed efficient DENV-2 replication. Among the nonhuman cell lines, nonhuman primate (Vero and LLC-MK2), pig (PK-15), rabbit (RK-13), hamster (BHK21) and chicken (DF-1) cell lines supported productive ZIKV replication. These animal species may be important reservoirs and/or potential animal models for ZIKV. The findings in our study help to explain the viral shedding pattern, transmission and pathogenesis of the rapidly disseminating ZIKV, and are useful for optimizing laboratory diagnostics and studies on the pathogenesis and counter-measures of ZIKV.
has issue date	2016-08-24(xsd:dateTime)
bibo:doi	10.1038/emi.2016.99
bibo:pmid	27553173
has license	cc-by
sha1sum (hex)	2782e0d4147b59228cff660820bf0b65c3417187
schema:url	https://doi.org/10.1038/emi.2016.99
resource representing a document's title	Differential cell line susceptibility to the emerging Zika virus: implications for disease pathogenesis, non-vector-borne human transmission and animal reservoirs
has PubMed Central identifier	PMC5034105
has PubMed identifier	27553173
schema:publication	Emerg Microbes Infect
resource representing a document's body	covid:2782e0d4147b59228cff660820bf0b65c3417187#body_text
is schema:about of	named entity 'rapidly' named entity 'CPE' named entity 'renal' named entity 'expression' named entity 'LNCaP' named entity 'manifestations' named entity 'NS1' named entity 'reservoirs' named entity 'MUSCLE' named entity 'NEUROLOGICAL' named entity 'PATHOGENESIS' named entity 'animal models' named entity 'optimizing' named entity 'complications' named entity 'tonsils' named entity 'flaviviruses' named entity 'ZIKV' named entity 'febrile illness' named entity 'DENV' named entity 'fetal calf serum' named entity 'HEK cells' named entity 'central nervous' named entity 'nasopharyngeal swabs' named entity 'pathogenesis' named entity 'cell lines' named entity 'ZIKV' named entity 'bat' named entity 'tropism' named entity 'neurological complications' named entity 'microglial activation' named entity 'gene' named entity 'TP2' named entity 'nonhuman primate' named entity 'viral load' named entity 'cerebral organoids' named entity 'protein' named entity 'Huh-7' named entity 'expression vector' named entity 'Zika Forest' named entity 'atrophy' named entity 'staining' named entity 'hamster' named entity 'cell lines' named entity 'type I interferon receptor' named entity 'Sigma-Aldrich' named entity 'DENV' named entity 'ZIKV' named entity 'ZIKV' named entity 'DENV' named entity 'neuronal' named entity 'sexual transmission' named entity 'type' named entity 'genital tract' named entity 'anatomical' named entity 'log' named entity 'ZIKV' named entity 'cytoplasmic' named entity 'ZIKV' named entity 'infection'

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