About: Proteomic analysis of sheep primary testicular cells infected with bluetongue virus

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Proteomic analysis of sheep primary testicular cells infected with bluetongue virus
Creator	Chang, Huiyun Du, Junzheng Gao, Shandian Liu, Guangyuan Luo, Jianxun Tian, Zhancheng Xing, Shanshan Yin, Hong Xie, Junren
Source	Medline; PMC
abstract	Bluetongue virus (BTV) causes a non‐contagious, arthropod‐transmitted disease in wild and domestic ruminants, such as sheep. In this study, we used iTRAQ labeling coupled with LC‐MS/MS for quantitative identification of differentially expressed proteins in BTV‐infected sheep testicular (ST) cells. Relative quantitative data were obtained for 4455 proteins in BTV‐ and mock‐infected ST cells, among which 101 and 479 proteins were differentially expressed at 24 and 48 h post‐infection, respectively, indicating further proteomic changes during the later stages of infection. Ten corresponding genes of differentially expressed proteins were validated via real‐time RT‐PCR. Expression levels of three representative proteins, eIF4a1, STAT1 and HSP27, were further confirmed via western blot analysis. Bioinformatics analysis disclosed that the differentially expressed proteins are primarily involved in biological processes related to innate immune response, signal transduction, nucleocytoplasmic transport, transcription and apoptosis. Several upregulated proteins were associated with the RIG‐I‐like receptor signaling pathway and endocytosis. To our knowledge, this study represents the first attempt to investigate proteome‐wide dysregulation in BTV‐infected cells with the aid of quantitative proteomics. Our collective results not only enhance understanding of the host response to BTV infection but also highlight multiple potential targets for the development of antiviral agents.
has issue date	2016-04-13(xsd:dateTime)
bibo:doi	10.1002/pmic.201500275
bibo:pmid	26989863
has license	no-cc
sha1sum (hex)	298b1fb8728be8cf99f6397b2bc4a472bb69915c
schema:url	https://doi.org/10.1002/pmic.201500275
resource representing a document's title	Proteomic analysis of sheep primary testicular cells infected with bluetongue virus
has PubMed Central identifier	PMC7168089
has PubMed identifier	26989863
schema:publication	Proteomics
resource representing a document's body	covid:298b1fb8728be8cf99f6397b2bc4a472bb69915c#body_text
is schema:about of	named entity 'cells' named entity 'indicating' named entity 'wild' named entity 'antiviral agents' named entity 'Bioinformatics' named entity '101' named entity 'TRANSMITTED' named entity 'EXPRESSED' named entity 'TO INVESTIGATE' named entity 'INNATE IMMUNE RESPONSE' named entity 'RELATIVE' named entity 'INDICATING' named entity 'QUANTITATIVE PROTEOMICS' named entity 'EXPRESSION' named entity 'UPREGULATED' named entity 'ANTIVIRAL AGENTS' named entity 'NUCLEOCYTOPLASMIC TRANSPORT' named entity 'AID' covid:arg/298b1fb8728be8cf99f6397b2bc4a472bb69915c named entity 'Bluetongue virus' named entity 'cells' named entity 'RIG-I-like receptor' named entity 'western blot' named entity 'involved' named entity 'quantitative proteomics' named entity 'HSP27' named entity 'innate immune response' named entity 'proteomic' named entity 'eIF4a1' named entity 'LC-MS/MS' named entity 'differentially expressed' named entity 'upregulated' named entity 'infection' named entity 'Bioinformatics' named entity 'transcription' named entity 'antiviral agents' named entity 'Bluetongue virus' named entity 'differentially expressed' named entity 'bluetongue virus' named entity 'testicular' named entity 'sheep' named entity 'molecular chaperones' named entity 'BHK-21 cells' named entity 'nucleolus' named entity 'African swine fever virus' named entity 'viral infection' named entity 'nucleus' named entity 'proteomic analysis' named entity 'time-of-flight' named entity 'hpi' named entity 'expression patterns' named entity 'infection' named entity 'sequenced' named entity 'hpi' named entity 'hpi' named entity 'host cell' named entity 'Japanese encephalitis' named entity 'viruses' named entity 'LC-MS/MS' named entity 'MASCOT' named entity 'receptor' named entity 'western blot' named entity 'type I IFN' named entity 'BHK-21 cells' named entity 'infection' named entity 'Applied Biosystems' named entity 'cell components' named entity 'signaling molecules' named entity 'immunomodulation' named entity 'co-expression' named entity 'Nup358' named entity 'virus' named entity 'virus'

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