About: Identification and subcellular localization of porcine deltacoronavirus accessory protein NS6

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Identification and subcellular localization of porcine deltacoronavirus accessory protein NS6 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Identification and subcellular localization of porcine deltacoronavirus accessory protein NS6
Creator	Fang, Liurong Wang, Dang Xiao, Shaobo Dong, Nan Fang, Puxian Hong, Yingying Liu, Xiaorong Bi, Jing Ma, Panpan Wang, Yongle
source	Elsevier; Medline; PMC
abstract	Abstract Porcine deltacoronavirus (PDCoV) is an emerging swine enteric coronavirus. Accessory proteins are genus-specific for coronavirus, and two putative accessory proteins, NS6 and NS7, are predicted to be encoded by PDCoV; however, this remains to be confirmed experimentally. Here, we identified the leader-body junction sites of NS6 subgenomic RNA (sgRNA) and found that the actual transcription regulatory sequence (TRS) utilized by NS6 is non-canonical and is located upstream of the predicted TRS. Using the purified NS6 from an Escherichia coli expression system, we obtained two anti-NS6 monoclonal antibodies that could detect the predicted NS6 in cells infected with PDCoV or transfected with NS6-expressing plasmids. Further studies revealed that NS6 is always localized in the cytoplasm of PDCoV-infected cells, mainly co-localizing with the endoplasmic reticulum (ER) and ER-Golgi intermediate compartments, as well as partially with the Golgi apparatus. Together, our results identify the NS6 sgRNA and demonstrate its expression in PDCoV-infected cells.
has issue date	2016-12-31(xsd:dateTime)
bibo:doi	10.1016/j.virol.2016.09.015
bibo:pmid	27661736
has license	els-covid
sha1sum (hex)	2ae71791c78a447e4d538eb42bad143bbb3c9f3a
schema:url	https://doi.org/10.1016/j.virol.2016.09.015
resource representing a document's title	Identification and subcellular localization of porcine deltacoronavirus accessory protein NS6
has PubMed Central identifier	PMC7111631
has PubMed identifier	27661736
schema:publication	Virology
resource representing a document's body	covid:2ae71791c78a447e4d538eb42bad143bbb3c9f3a#body_text
is schema:about of	named entity 'transfected' named entity 'predicted' named entity 'enteric' named entity 'Accessory' named entity 'coronavirus' named entity 'endoplasmic reticulum' named entity 'monoclonal antibodies' named entity 'sgRNA' named entity 'transfected' named entity 'protein' named entity 'monoclonal antibodies' named entity 'De Haan' named entity 'IgG' named entity 'cDNA' named entity 'intraperitoneal injection' named entity 'virus replication' named entity 'regulatory sequence' named entity 'peptide' named entity 'Invitrogen' named entity 'vector' named entity 'hpi' named entity 'fetal bovine serum' named entity 'gene' named entity 'western blotting' named entity 'Alexa Fluor' named entity 'antibody' named entity 'cell lysis' named entity 'viral structural proteins' named entity 'coronaviruses' named entity 'coronavirus' named entity 'cloned' named entity 'inclusion bodies' named entity 'Trizol' named entity 'Coronaviridae' named entity 'p3b' named entity 'BALB/c' named entity 'cDNAs' named entity 'inoculation' named entity 'amino acid' named entity 'cell lysates' named entity 'hemagglutinin' named entity 'cloning' named entity 'protein' named entity 'radioactive labeling' named entity 'MAb' named entity 'protein' named entity 'expression vector' named entity 'cDNA' named entity 'cytoplasm' named entity 'coronaviruses' named entity 'p3a' named entity 'protein' named entity 'ER-Golgi intermediate compartment' named entity 'viral replication' named entity 'Korea' named entity 'sgRNA' named entity 'polyclonal antibody' named entity 'proteolytically cleaved' named entity 'vector' named entity 'immunization' named entity 'Nucleocapsid' named entity 'protein' named entity 'MAbs' named entity 'China' named entity 'Ohio' named entity 'interferon' named entity 'genome' named entity 'nitrocellulose' named entity 'viral genome' named entity 'transcription' named entity 'gammacoronavirus' named entity 'coronavirus'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software