About: The use of RT-PCR for determination of separate end-points for the strains IB H120 and IB D274 in titration of the combination vaccine Poulvac IB(®) primer

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About: The use of RT-PCR for determination of separate end-points for the strains IB H120 and IB D274 in titration of the combination vaccine Poulvac IB(®) primer Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The use of RT-PCR for determination of separate end-points for the strains IB H120 and IB D274 in titration of the combination vaccine Poulvac IB(®) primer
Creator	Duyves, W Geerligs, H Meinders, C Snel, J
Source	Elsevier; Medline; PMC
abstract	Poulvac IB(®) Primer is a lyophilized vaccine containing two attenuated infectious bronchitis strains in one vial, IB H120 and IB D274. For quantification of the viral content of the vaccine, dilution series of the final product are inoculated in embryonated chicken eggs. After the incubation period of seven days standard practice is for the embryos to be taken from each egg and examined visually for IB specific lesions; these readings are used to determine an end-point in viral titrations. The result is a titre value to which both strains contribute. However, it is not clear what the live virus titre is for strain IB H120 and for strain IB D274. In order to determine end-points in the titration for each of the two strains, we collected the allantoic fluids from each egg after the incubation period and tested these for the presence of IB H120 and IB D274 by a strain specific reverse phase PCR. Based on the data obtained by PCR we were able to determine an end-point for each of the two strains. For a given commercial batch of Poulvac IB primer we determined titres of 10(6.31) EID(50) per vial for IB H120 and 10(6.59) EID(50) for IB D274 using PCR for end-point determination. These end-points matched well with the end-point determined for both strains cumulatively after visual examination, i.e. 10(6.67) EID(50) per vial. It is concluded that PCR is a suitable means to determine end-points in titrations of live viruses.
has issue date	2013-07-01(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2013.06.029
bibo:pmid	23827949
has license	no-cc
sha1sum (hex)	2ca4dea2e06fcb52b003a52eb3bd502c964e3da5
schema:url	https://doi.org/10.1016/j.jviromet.2013.06.029
resource representing a document's title	The use of RT-PCR for determination of separate end-points for the strains IB H120 and IB D274 in titration of the combination vaccine Poulvac IB(®) primer
has PubMed Central identifier	PMC7119798
has PubMed identifier	23827949
schema:publication	J Virol Methods
resource representing a document's body	covid:2ca4dea2e06fcb52b003a52eb3bd502c964e3da5#body_text
is schema:about of	named entity 'COMBINATION VACCINE' named entity 'PCR' named entity 'lesions' named entity 'titre' named entity 'incubation period' named entity 'DETERMINATION' named entity 'POINTS' covid:arg/2ca4dea2e06fcb52b003a52eb3bd502c964e3da5 named entity 'IS A' named entity 'USE OF' named entity 'COLLECTED' named entity 'MEANS' named entity 'LESIONS' named entity 'LIVE' named entity 'SERIES' named entity 'BASED' named entity 'QUANTIFICATION' named entity 'OBTAINED BY' named entity 'VACCINE' named entity 'END-POINT' named entity 'FLUIDS' named entity 'DATA' named entity 'CONTENT' named entity 'THESE' named entity 'PRIMER' named entity 'STRAIN' named entity 'SEPARATE' named entity 'USED' named entity 'EGG' named entity 'VIRUSES' named entity 'STANDARD' named entity 'USING' named entity 'STRAINS' named entity 'TITRATION' named entity 'VIRAL' named entity 'EMBRYOS' named entity 'CONTAINING' named entity 'WHAT' named entity 'INFECTIOUS BRONCHITIS' named entity 'RESULT' named entity 'DETERMINATION' named entity 'TITRATIONS' named entity '6.67' named entity 'PHASE' named entity 'CHICKEN' named entity 'POINTS' named entity 'SPECIFIC' named entity 'PRIMER' named entity 'STRAINS' named entity 'END' named entity 'RT-PCR' named entity 'TITRATION' named entity 'ATTENUATED' named entity 'SEVEN' named entity 'TESTED' named entity 'VIAL' named entity 'PRESENCE OF' named entity 'SUITABLE' named entity 'CLEAR' named entity 'READINGS' named entity 'MATCHED' named entity 'COMMERCIAL BATCH' named entity 'TAKEN' named entity 'DILUTION' named entity 'TITRE' named entity 'END' named entity 'REVERSE' named entity 'CONTRIBUTE' named entity 'FINAL' named entity 'PRODUCT' named entity 'PCR' named entity 'EGGS' named entity 'VIRUS' named entity 'DETERMINE' named entity 'VALUE' named entity 'PRACTICE' named entity 'VISUAL EXAMINATION' named entity 'EXAMINED'

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