About: Development of a multiplex one step RT-PCR that detects eighteen respiratory viruses in clinical specimens and comparison with real time RT-PCR

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About: Development of a multiplex one step RT-PCR that detects eighteen respiratory viruses in clinical specimens and comparison with real time RT-PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development of a multiplex one step RT-PCR that detects eighteen respiratory viruses in clinical specimens and comparison with real time RT-PCR
Creator	Potdar, Varsha Heydari, Mostafa Rane, Grishma Chadha, Mandeep Anand, Siddharth Choudhary, Manohar Mishra, Akhilesh
source	Elsevier; Medline; PMC
abstract	Rapid and accurate diagnosis of viral respiratory infections is crucial for patient management. Multiplex reverse transcriptase polymerase chain reaction (mRT-PCR) is used increasingly to diagnose respiratory infections and has shown to be more sensitive than viral culture and antigen detection. Objective of the present study was to develop a one-step mRT-PCR that could detect 18 respiratory viruses in three sets. The method was compared with real time RT-PCR (rRT-PCR) for its sensitivity and specificity. Clinical specimens from 843 pediatric patients with respiratory symptoms were used in the study. 503 (59.7%) samples were detected positive by mRT-PCR. Of these 462 (54.8%) exhibited presence of a single pathogen and 41 (4.9%) had multiple pathogens. rRT-PCR detected 439 (52.1%) positive samples, where 419 (49.7%) exhibited one virus and 20 (2.4%) showed co-infections. Concordance between mRT-PCR and rRT-PCR was 91.9% and kappa correlation 0.837. Sensitivity and specificity of mRT-PCR were 99.5% and 83.7% while that of rRT-PCR was 86.9% and 99.4% respectively. Rhinovirus (17.2%) was the most frequently detected virus followed by respiratory syncytial virus B (15.4%), H1N1pdm09 (8.54%), parainfluenza virus-3 (5.8%) and metapneumovirus (5.2%). In conclusion, mRT-PCR is a rapid, cost effective, specific and highly sensitive method for detection of respiratory viruses.
has issue date	2013-01-08(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2012.12.017
bibo:pmid	23313883
has license	no-cc
sha1sum (hex)	2d5f0773a3e949292d246854d7532ef2885ebdba
schema:url	https://doi.org/10.1016/j.jviromet.2012.12.017
resource representing a document's title	Development of a multiplex one step RT-PCR that detects eighteen respiratory viruses in clinical specimens and comparison with real time RT-PCR
has PubMed Central identifier	PMC7119668
has PubMed identifier	23313883
schema:publication	J Virol Methods
resource representing a document's body	covid:2d5f0773a3e949292d246854d7532ef2885ebdba#body_text
is schema:about of	named entity 'ONE STEP' named entity 'Multiplex' named entity 'viral culture' named entity 'respiratory' named entity 'RT-PCR' named entity 'DEVELOPMENT' named entity 'MULTIPLEX' named entity 'STUDY' named entity 'EFFECTIVE' named entity 'SENSITIVITY AND SPECIFICITY' named entity '837' named entity 'RESPIRATORY SYMPTOMS' named entity 'PATIENTS' named entity '462' named entity 'PCR' named entity 'HIGHLY SENSITIVE' named entity 'FREQUENTLY' covid:arg/2d5f0773a3e949292d246854d7532ef2885ebdba named entity 'PATHOGENS' named entity 'MULTIPLE' named entity 'SAMPLES' named entity 'COMPARED' named entity 'METHOD' named entity '2849' named entity '99.5' named entity 'THESE' named entity 'RAPID' named entity 'STEP' named entity 'ACCURATE' named entity 'SINGLE' named entity 'PRESENT' named entity 'RT-PCR' named entity 'RESPIRATORY VIRUSES' named entity 'VIRAL CULTURE' named entity 'PATIENT MANAGEMENT' named entity 'DETECTION' named entity 'COST' named entity 'COMPARISON' named entity 'REAL TIME RT-PCR' named entity 'SPECIMENS' named entity 'FOR PATIENT' named entity 'RESPIRATORY SYNCYTIAL VIRUS B' named entity 'KAPPA' named entity 'RESPIRATORY VIRUSES' named entity 'RRT-PCR' named entity 'CONCORDANCE' named entity '288' named entity 'DIAGNOSE' named entity 'MULTIPLEX' named entity 'EIGHTEEN' named entity 'CLINICAL' named entity 'DETECTED' named entity 'RESPIRATORY INFECTIONS' named entity 'CLINICAL' named entity 'FOLLOWED BY' named entity 'REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION' named entity 'DIAGNOSIS' named entity 'METAPNEUMOVIRUS' named entity 'WHERE' named entity '282' named entity 'RHINOVIRUS' named entity 'DETECT' named entity 'SPECIMENS' named entity 'REAL TIME RT-PCR' named entity 'USED' named entity 'SENSITIVE' named entity 'VIRAL' named entity 'PARAINFLUENZA VIRUS' named entity 'ITS' named entity 'IS A' named entity 'ANTIGEN DETECTION' named entity 'SPECIFIC' named entity 'CORRELATION' named entity 'VIRUS' named entity 'POSITIVE'

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