About: Citrus tristeza virus co-opts glyceraldehyde 3-phosphate dehydrogenase for its infectious cycle by interacting with the viral-encoded protein p23

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About: Citrus tristeza virus co-opts glyceraldehyde 3-phosphate dehydrogenase for its infectious cycle by interacting with the viral-encoded protein p23 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Citrus tristeza virus co-opts glyceraldehyde 3-phosphate dehydrogenase for its infectious cycle by interacting with the viral-encoded protein p23
Creator	Flores, Ricardo Moreno, Pedro Navarro, Luis Peña, Leandro Ruiz-Ruiz, Susana Spanò, Roberta
Source	Medline; PMC
abstract	KEY MESSAGE: Citrus tristeza virus encodes a unique protein, p23, with multiple functional roles that include co-option of the cytoplasmic glyceraldehyde 3-phosphate dehydrogenase to facilitate the viral infectious cycle. ABSTRACT: The genome of citrus tristeza virus (CTV), genus Closterovirus family Closteroviridae, is a single-stranded (+) RNA potentially encoding at least 17 proteins. One (p23), an RNA-binding protein of 209 amino acids with a putative Zn-finger and some basic motifs, displays singular features: (i) it has no homologues in other closteroviruses, (ii) it accumulates mainly in the nucleolus and Cajal bodies, and in plasmodesmata, and (iii) it mediates asymmetric accumulation of CTV RNA strands, intracellular suppression of RNA silencing, induction of some CTV syndromes and enhancement of systemic infection when expressed as a transgene ectopically or in phloem-associated cells in several Citrus spp. Here, a yeast two-hybrid screening of an expression library of Nicotiana benthamiana (a symptomatic experimental host for CTV), identified a transducin/WD40 domain protein and the cytosolic glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as potential host interactors with p23. Bimolecular fluorescence complementation corroborated the p23-GAPDH interaction in planta and showed that p23 interacts with itself in the nucleolus, Cajal bodies and plasmodesmata, and with GAPDH in the cytoplasm (forming aggregates) and in plasmodesmata. The latter interaction was preserved in a p23 deletion mutant affecting the C-terminal domain, but not in two others affecting the Zn-finger and one internal basic motif. Virus-induced gene silencing of GAPDH mRNA resulted in a decrease of CTV titer as revealed by real-time RT-quantitative PCR and RNA gel-blot hybridization. Thus, like other viruses, CTV seems to co-opt GAPDH, via interaction with p23, to facilitate its infectious cycle.
has issue date	2018-11-03(xsd:dateTime)
bibo:doi	10.1007/s11103-018-0783-0
bibo:pmid	30392159
has license	no-cc
sha1sum (hex)	2ddceef7fc5e3dce7e3b7f716b74735c8c8d2e1f
schema:url	https://doi.org/10.1007/s11103-018-0783-0
resource representing a document's title	Citrus tristeza virus co-opts glyceraldehyde 3-phosphate dehydrogenase for its infectious cycle by interacting with the viral-encoded protein p23
has PubMed Central identifier	PMC7088584
has PubMed identifier	30392159
schema:publication	Plant Mol Biol
resource representing a document's body	covid:2ddceef7fc5e3dce7e3b7f716b74735c8c8d2e1f#body_text
is schema:about of	named entity 'amino acids' named entity 'strands' named entity 'p23' named entity 'transducin' named entity 'tristeza virus' named entity 'FEATURES' named entity 'SYMPTOMATIC' named entity 'GEL' named entity 'MEDIATES' named entity 'KEY' named entity '28A' named entity 'ENHANCEMENT' named entity 'DELETION' named entity 'OPTION' named entity 'SPP' named entity 'CTV' named entity 'AFFECTING' named entity 'Bimolecular fluorescence complementation' named entity 'single-stranded' named entity 'RNA' named entity 'The' named entity 'GAPDH' named entity 'Thus' named entity 'GAPDH' named entity 'cytoplasm' named entity 'identified' named entity 'silencing' named entity 'RNA-binding protein' named entity 'interaction' named entity 'viruses' named entity 'Citrus' named entity 'GAPDH' named entity 'CTV' named entity 'motifs' named entity 'message' named entity 'yeast two-hybrid screening' named entity 'Closteroviridae' named entity 'CTV' named entity 'GAPDH' named entity 'Citrus tristeza virus' named entity 'Citrus' named entity 'hybridization' named entity 'Bimolecular fluorescence complementation' named entity 'plasmodesmata' named entity 'nucleolus' named entity 'phloem' named entity 'motif' named entity 'protein' named entity 'CTV' named entity 'RNA' named entity 'cytoplasmic' named entity 'RNA' named entity 'Closterovirus' named entity 'Cajal bodies' named entity 'cytosolic' named entity 'transducin' named entity 'CTV' named entity 'plasmodesmata' named entity 'p23' named entity 'Citrus tristeza virus' named entity 'plasmids' named entity 'GAPDH' named entity 'cell movement' named entity 'nucleic acid' named entity 'transducin' named entity 'Y2H' named entity 'N. benthamiana' named entity 'fluorescence' named entity 'Control experiments' named entity 'red clover' named entity 'CTV' named entity 'protein' named entity 'primers' named entity 'TBSV' named entity 'N. benthamiana' named entity 'restriction sites'

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