About: Detection and characterization of viruses as field and vaccine strains in feedlot cattle with bovine respiratory disease

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About: Detection and characterization of viruses as field and vaccine strains in feedlot cattle with bovine respiratory disease Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection and characterization of viruses as field and vaccine strains in feedlot cattle with bovine respiratory disease
Creator	Smith, R Burge, L Fulton, R Ridpath, J Chase, C Clement, T Confer, A D'offay, J Eberle, R Landis, C Miles, D Neill, J Payton, M Saliki, J
Source	Elsevier; Medline; PMC
abstract	Abstract This study investigated viruses in bovine respiratory disease (BRD) cases in feedlots, including bovine herpesvirus-1 (BoHV-1), bovine viral diarrhea virus (BVDV), bovine respiratory syncytial virus (BRSV), bovine coronaviruses (BoCV) and parainfluenza-3 virus (PI3V). Nasal swabs were collected from 114 cattle on initial BRD treatment. Processing included modified live virus (MLV) vaccination. Seven BRD necropsy cases were included for 121 total cases. Mean number of days on feed before first sample was 14.9 days. Swabs and tissue homogenates were tested by gel based PCR (G-PCR), quantitative-PCR (qPCR) and quantitative real time reverse transcriptase PCR (qRT-PCR) and viral culture. There were 87/114 (76.3%) swabs positive for at least one virus by at least one test. All necropsy cases were positive for at least one virus. Of 121 cases, positives included 18/121 (14.9%) BoHV-1; 19/121 (15.7%) BVDV; 76/121 (62.8%) BoCV; 11/121 (9.1%) BRSV; and 10/121 (8.3%) PI3V. For nasal swabs, G-PCR (5 viruses) detected 44/114 (38.6%); q-PCR and qRT-PCR (4 viruses) detected 81/114 (71.6%); and virus isolation detected 40/114 (35.1%). Most were positive for only one or two tests, but not all three tests. Necropsy cases had positives: 5/7 G-PCR, 5/7 q-PCR and qRT-PCR, and all were positive by cell culture. In some cases, G-PCR and both real time PCR were negative for BoHV-1, BVDV, and PI3V in samples positive by culture. PCR did not differentiate field from vaccines strains of BoHV-1, BVDV, and PI3V. However based on sequencing and analysis, field and vaccine strains of culture positive BoHV-1, BoCV, BVDV, and PI3V, 11/18 (61.1%) of BoHV-1 isolates, 6/17 (35.3%) BVDV isolates, and 1/10 (10.0%) PI3V identified as vaccine. BRSV was only identified by PCR testing. Interpretation of laboratory tests is appropriate as molecular based tests and virus isolation cannot separate field from vaccine strains. Additional testing using sequencing appears appropriate for identifying vaccine strains.
has issue date	2016-06-24(xsd:dateTime)
bibo:doi	10.1016/j.vaccine.2016.04.020
bibo:pmid	27108192
has license	els-covid
sha1sum (hex)	2f6470175fa154e8c272bcf522ff6305d532e378
schema:url	https://doi.org/10.1016/j.vaccine.2016.04.020
resource representing a document's title	Detection and characterization of viruses as field and vaccine strains in feedlot cattle with bovine respiratory disease
has PubMed Central identifier	PMC7173208
has PubMed identifier	27108192
schema:publication	Vaccine
resource representing a document's body	covid:2f6470175fa154e8c272bcf522ff6305d532e378#body_text
is schema:about of	named entity 'NECROPSY' named entity 'LABORATORY TESTS' named entity 'BOVINE' named entity 'COLLECTED' named entity 'CATTLE' named entity 'STUDY' named entity 'MOLECULAR' covid:arg/2f6470175fa154e8c272bcf522ff6305d532e378 named entity 'cattle' named entity 'cell culture' named entity 'vaccines' named entity 'BVDV' named entity 'PCR' named entity 'coronaviruses' named entity 'q-PCR' named entity 'BRD' named entity 'necropsy' named entity 'PCR' named entity 'BVDV' named entity 'reverse transcriptase PCR' named entity 'PCR' named entity 'virus' named entity 'feedlot' named entity 'q-PCR' named entity 'cattle' named entity 'necropsy' named entity 'q-PCR' named entity 'vaccine' named entity 'bovine' named entity 'buffy coat' named entity 'PCR' named entity 'cell culture' named entity 'pathogen' named entity 'immunofluorescence' named entity 'viruses' named entity 'PCR' named entity 'real time PCR' named entity 'PCR primers' named entity 'vaccination' named entity 'Virus isolation' named entity 'viruses' named entity 'gold standard' named entity 'BVDV' named entity 'Necropsy' named entity 'Histophilus somni' named entity 'BVDV' named entity 'viruses' named entity '3, 4' named entity 'U.S. Department of Agriculture' named entity 'q-PCR' named entity 'cattle' named entity 'q-PCR' named entity 'bovine' named entity 'BVDV' named entity 'feedlot' named entity 'vaccine' named entity 'viruses' named entity 'PCR' named entity 'Viral' named entity 'phylogenetic analysis' named entity 'BVDV' named entity 'protein' named entity 'viruses' named entity 'genetic analysis' named entity 'diagnostic tests' named entity 'NS5B' named entity 'live animals' named entity 'PCR'

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