About: The amino and carboxyl domains of the infectious bronchitis virus nucleocapsid protein interact with 3′ genomic RNA

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About: The amino and carboxyl domains of the infectious bronchitis virus nucleocapsid protein interact with 3′ genomic RNA Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The amino and carboxyl domains of the infectious bronchitis virus nucleocapsid protein interact with 3′ genomic RNA
Creator	Collisson, Ellen Zhou, Minglong
Source	Elsevier; Medline; PMC
abstract	Abstract Previous studies indicated that the nucleocapsid (N) protein of infectious bronchitis virus (IBV) interacted with specific sequences in the 3′ non-coding region of IBV RNA. In order to identify domains in the N protein that bind to RNA, the whole protein (409 amino acids) and six overlapping fragments were expressed as fusion polypeptides with six histidine-tags. Using gel shift assays, the intact N protein and amino polypeptides, from residues 1 to 171 and residues 1 to 274, and carboxyl polypeptides, extending from residues 203 to 409 and residues 268 to 407, were found to interact with positive-stranded IBV RNA representing the 3′ end of the genome. The two 32P-labeled probes that interacted with N and the amino and carboxyl fragments of N were RNA consisting of the IBV N gene and adjacent 3′ non-coding terminus, and RNA consisting of the 155-nucleotide sequences at the 3′ end of the 504-nt 3′ untranslated region. In contrast, the polypeptide fragment from the middle region, residues 101–283, did not interact with these 3′ IBV RNAs. The binding site in the amino region of N was either not present or only partially present in the first 91 residues because no interaction with RNA was observed with the polypeptide incorporating these residues. Cache Valley virus N expressed with a histidine tag, bovine serum albumin, and the basic lysozyme protein did not shift the IBV RNA. The lower molarities of the carboxyl fragment compared with residue 1–274 fragment needed for equivalent shifts suggested that the binding avidity for RNA at the carboxyl domain was greater than the amino domain.
has issue date	2000-03-31(xsd:dateTime)
bibo:doi	10.1016/s0168-1702(00)00126-x
bibo:pmid	10773316
has license	els-covid
sha1sum (hex)	2f67fbe3a031298155baeb6a73e83bb47ddc97d4
schema:url	https://doi.org/10.1016/s0168-1702%2800%2900126-x
resource representing a document's title	The amino and carboxyl domains of the infectious bronchitis virus nucleocapsid protein interact with 3′ genomic RNA
has PubMed Central identifier	PMC7125745
has PubMed identifier	10773316
schema:publication	Virus Research
resource representing a document's body	covid:2f67fbe3a031298155baeb6a73e83bb47ddc97d4#body_text
is schema:about of	named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'domain' named entity 'protein' named entity 'polypeptide' named entity 'residues' named entity 'residues' named entity 'protein' named entity 'amino' named entity 'amino acids' named entity 'virus' named entity 'nucleocapsid' named entity 'RNA' named entity 'interact' named entity 'RNA' named entity 'REGION' named entity 'GENOME' named entity 'COMPARED' named entity 'TO INTERACT WITH' named entity 'LOWER' named entity 'BINDING SITE' named entity 'NOT PRESENT' named entity 'DOMAIN' named entity 'THESE' named entity 'OBSERVED' named entity 'BIND' named entity 'MIDDLE' named entity 'HISTIDINE TAG' named entity 'PARTIALLY' named entity '504' named entity 'POLYPEPTIDE' named entity 'PROTEIN ' named entity 'OVERLAPPING' named entity 'FRAGMENT' named entity '101' named entity 'RESIDUE' named entity 'INDICATED' named entity 'ORDER' named entity 'UNTRANSLATED REGION' named entity 'NUCLEOCAPSID' named entity 'POSITIVE' named entity 'LABELED' named entity 'INTERACT' named entity 'INTERACTED' named entity 'BOVINE SERUM ALBUMIN' named entity 'INTERACTION' named entity 'INTERACT' named entity 'USING' named entity 'CACHE VALLEY VIRUS' named entity 'N GENE' named entity 'NUCLEOTIDE SEQUENCES' named entity 'CODING REGION' named entity '283' named entity 'GREATER THAN' named entity 'CONTRAST' named entity 'FOUND' named entity 'PROBES' named entity 'EITHER' named entity 'CARBOXYL' named entity 'HISTIDINE' named entity 'NUCLEOCAPSID PROTEIN' named entity 'AMINO' named entity 'END' named entity 'AMINO' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'PROTEIN ' named entity 'CARBOXYL' named entity 'STUDIES' named entity 'EXPRESSED' named entity 'DID' named entity 'NEEDED' named entity 'BINDING' named entity 'TERMINUS' named entity '268' named entity 'AVIDITY' named entity 'BASIC' named entity 'POLYPEPTIDES' named entity 'NON-' named entity 'SPECIFIC' named entity 'EXTENDING' named entity '203'

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