About: Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs

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About: Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs
Creator	Thiel, Volker Eriksson, Klara
topic	covid:2f9aa31ee54869a77d079a62f389a541914b763d#this
Source	Medline; PMC
abstract	Coronavirus reverse genetic systems have become valuable tools for studying the molecular biology of coronavirus infections. They have been applied to the generation of recombinant coronaviruses, selectable replicon RNAs, and coronavirus-based vectors for heterologous gene expression. Here we provide a collection of protocols for the generation, cloning, and modification of full-length coronavirus cDNA using vaccinia virus as a cloning vector. Based on cloned coronaviral cDNA, we describe the generation of recombinant coronaviruses and stable cell lines containing coronaviral replicon RNAs. Initially, the vaccinia virus-based reverse genetic system was established for the generation of recombinant human coronavirus 229E. However, it is also applicable to the generation of other coronaviruses, such as the avian infectious bronchitis virus, mouse hepatitis virus, and SARS coronavirus.
has issue date	2007-11-28(xsd:dateTime)
bibo:doi	10.1007/978-1-59745-181-9_18
bibo:pmid	19057873
has license	no-cc
sha1sum (hex)	2f9aa31ee54869a77d079a62f389a541914b763d
schema:url	https://doi.org/10.1007/978-1-59745-181-9_18
resource representing a document's title	Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs
has PubMed Central identifier	PMC7121376
has PubMed identifier	19057873
schema:publication	SARS- and Other Coronaviruses
resource representing a document's body	covid:2f9aa31ee54869a77d079a62f389a541914b763d#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/2f9aa31ee54869a77d079a62f389a541914b763d
is schema:about of	named entity 'provide' named entity 'replicon' named entity 'recombinant' named entity 'GENERATION' named entity 'REPLICON' named entity 'CLONING VECTOR' named entity 'CDNA' named entity 'MOLECULAR BIOLOGY' named entity 'RNAS' named entity 'ESTABLISHED' named entity 'SYSTEM' named entity 'PROVIDE' named entity 'AVIAN INFECTIOUS BRONCHITIS VIRUS' named entity 'APPLIED' named entity 'RNAS' named entity 'TOOLS' named entity 'HERE' named entity 'CORONAVIRUS' named entity 'CELL LINES' named entity 'SYSTEMS' named entity 'GENERATION' named entity 'REVERSE' named entity 'HAVE' named entity 'CORONAVIRUS INFECTIONS' named entity 'VACCINIA VIRUS' named entity 'HETEROLOGOUS GENE EXPRESSION' named entity 'BASED' named entity 'LENGTH' named entity 'DESCRIBE' named entity 'CORONAVIRUSES' named entity 'RECOMBINANT' named entity 'PROTOCOLS' named entity 'CELL LINES' named entity 'STABLE' named entity 'CONTAINING' named entity 'VECTORS' named entity 'HUMAN CORONAVIRUS 229E' named entity 'REPLICON' named entity 'APPLICABLE' named entity 'USING' named entity 'USING' named entity 'RECOMBINANT' named entity 'VACCINIA VIRUS' named entity 'MODIFICATION' named entity 'CLONING' named entity 'CLONING VECTOR' named entity 'INITIALLY' named entity 'SARS CORONAVIRUS' named entity 'STABLE' named entity 'COLLECTION' named entity 'GENETIC' named entity 'MOUSE HEPATITIS VIRUS' named entity 'CORONAVIRUSES' named entity 'CONTAINING' named entity 'CLONED' named entity 'replicon' named entity 'They' named entity 'cDNA' named entity 'expression' named entity 'coronavirus' named entity 'coronavirus' named entity 'biology' named entity 'reverse' named entity 'RNAs' named entity 'stable' named entity 'infections' named entity 'recombinant' named entity 'vaccinia virus' named entity 'SARS coronavirus' named entity 'cloned' named entity 'recombinant' named entity 'cloning vector' named entity 'replicon' named entity 'Coronaviruses' named entity 'Cell Lines' named entity 'ultracentrifuge' named entity 'UV light' named entity 'coronavirus'

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