About: The calcium-dependent ribonuclease XendoU promotes ER network formation through local RNA degradation

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About: The calcium-dependent ribonuclease XendoU promotes ER network formation through local RNA degradation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The calcium-dependent ribonuclease XendoU promotes ER network formation through local RNA degradation
Creator	Blower, Michael Schwarz, Dianne
Source	PMC
abstract	How cells shape and remodel organelles in response to cellular signals is a poorly understood process. Using Xenopus laevis egg extract, we found that increases in cytosolic calcium lead to the activation of an endogenous ribonuclease, XendoU. A fraction of XendoU localizes to the endoplasmic reticulum (ER) and is required for nuclear envelope assembly and ER network formation in a catalysis-dependent manner. Using a purified vesicle fusion assay, we show that XendoU functions on the surface of ER membranes to promote RNA cleavage and ribonucleoprotein (RNP) removal. Additionally, RNA removal from the surface of vesicles by RNase treatment leads to increased ER network formation. Using human tissue culture cells, we found that hEndoU localizes to the ER, where it promotes the formation of ER tubules in a catalysis-dependent manner. Together, these results demonstrate that calcium-activated removal of RNA from membranes by XendoU promotes and refines ER remodeling and the formation of tubular ER.
has issue date	2014-10-13(xsd:dateTime)
bibo:doi	10.1083/jcb.201406037
bibo:pmid	25287301
has license	cc-by-nc-sa
sha1sum (hex)	2ffacfd58f57a95344119c15d27560cdeaea2285
schema:url	https://doi.org/10.1083/jcb.201406037
resource representing a document's title	The calcium-dependent ribonuclease XendoU promotes ER network formation through local RNA degradation
has PubMed Central identifier	PMC4195833
has PubMed identifier	25287301
schema:publication	J Cell Biol
resource representing a document's body	covid:2ffacfd58f57a95344119c15d27560cdeaea2285#body_text
is schema:about of	named entity 'RIBOSOMAL RNA' named entity 'SNORNA' named entity 'ESIRNA' named entity 'ABBREVIATIONS' named entity 'cytostatic' named entity 'ribosomal RNA' named entity 'LOCAL' named entity 'FORMATION' named entity 'RNA DEGRADATION' named entity 'NETWORK' named entity 'CALCIUM' named entity 'DEPENDENT' named entity 'SIRNA' named entity 'PAPER' named entity 'LIGHT' named entity 'RIBONUCLEASE' named entity 'SMALL NUCLEOLAR RNA' named entity 'RRNA' named entity 'CYTOSTATIC FACTOR' named entity 'CSF' named entity 'USED' named entity 'MEMBRANE' named entity 'WASHED' named entity 'snoRNA' named entity 'wlm' named entity 'ribosomal RNA' named entity 'CSF' named entity 'cytostatic' named entity 'ribonuclease' named entity 'vesicle fusion' named entity 'MgCl' named entity 'ribosomes' named entity 'meiotic' named entity 'fertilization' named entity 'neuronal cells' named entity 'agarose beads' named entity 'heparin' named entity 'rRNA' named entity 'mRNAs' named entity 'Mass spectrometry' named entity 'Wollert' named entity 'mitosis' named entity 'nuclear envelope' named entity 'antibodies' named entity 'RNA' named entity 'BamHI' named entity 'nucleases' named entity 'ribosomal RNAs' named entity 'QIAGEN' named entity 'Ribonuclease' named entity 'reticulons' named entity 'siRNAs' named entity 'wild-type' named entity 'Heparin' named entity 'morphology' named entity 'RNA-seq' named entity 'PCR' named entity 'calcium' named entity 'tubule formation' named entity 'nuclease' named entity 'serum' named entity 'phenotype' named entity 'cleavage' named entity 'Histone H1' named entity 'calcium' named entity 'nuclear envelope' named entity 'IgG' named entity 'centrifugation' named entity 'Sigma-Aldrich' named entity 'GTP' named entity 'Coomassie blue' named entity 'glycerol' named entity 'positively charged' named entity 'staining' named entity 'Western blots' named entity 'Antibody' named entity 'Tholey' named entity 'CSF' named entity 'MetaMorph' named entity 'vesicle fusion'

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