About: MiR-10a-5p-Mediated Syndecan 1 Suppression Restricts Porcine Hemagglutinating Encephalomyelitis Virus Replication

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About: MiR-10a-5p-Mediated Syndecan 1 Suppression Restricts Porcine Hemagglutinating Encephalomyelitis Virus Replication Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	MiR-10a-5p-Mediated Syndecan 1 Suppression Restricts Porcine Hemagglutinating Encephalomyelitis Virus Replication
Creator	Gao, Feng He, Wenqi Lan, Yungang Zhao, Kui Li, Zi Guan, Jiyu Hu, Shiyu Chu, Dianfeng Fan, Gencheng Guo, Yuguang Medigeshi, Guruprasad
Source	Medline; PMC
abstract	Porcine hemagglutinating encephalomyelitis virus (PHEV) is a single-stranded RNA coronavirus that causes nervous dysfunction in the infected hosts and leads to widespread alterations in the host transcriptome by modulating specific microRNA (miRNA) levels. MiRNAs contribute to RNA virus pathogenesis by promoting antiviral immune response, enhancing viral replication, or altering miRNA-mediated host gene regulation. Thus, exploration of the virus–miRNA interactions occurring in PHEV-infected host may lead to the identification of novel mechanisms combating the virus life cycle or pathogenesis. Here, we discovered that the expression of miR-10a-5p was constitutively up-regulated by PHEV in both the N2a cells in vitro and mice brain in vivo. Treatment with miR-10a-5p mimics allowed miR-10a-5p enrichment and resulted in a significant restriction in PHEV replication, suggesting widespread negative regulation of the RNA virus infection by miR-10a-5p. The outcomes were also evidenced by miR-10a-5p inhibitor over-expression. Luciferase reporter, quantitative real-time PCR (qRT-PCR), and western blotting analysis further showed that Syndecan 1 (SDC1), a cell surface proteoglycan associated with host defense mechanisms, acts as a target gene of miR-10a-5p during PHEV infection. Naturally, siRNA-mediated knockdown of SDC1 leads to a reduction in viral replication, implying that SDC1 expression is likely a favorable condition for viral replication. Together, the findings demonstrated that the abundant miR-10a-5p leads to downstream suppression of SDC1, and it functions as an antiviral mechanism in the PHEV-induced disease, providing a potential strategy for the prevention and treatment of PHEV infection in the future work.
has issue date	2020-02-20(xsd:dateTime)
bibo:doi	10.3389/fmicb.2020.00105
bibo:pmid	32153518
has license	cc-by
sha1sum (hex)	3078165241b24fed34596efa9f563661f1caa00c
schema:url	https://doi.org/10.3389/fmicb.2020.00105
resource representing a document's title	MiR-10a-5p-Mediated Syndecan 1 Suppression Restricts Porcine Hemagglutinating Encephalomyelitis Virus Replication
has PubMed Central identifier	PMC7044266
has PubMed identifier	32153518
schema:publication	Front Microbiol
resource representing a document's body	covid:3078165241b24fed34596efa9f563661f1caa00c#body_text
is schema:about of	named entity 'CITATION' named entity 'RNA virus' named entity 'host' named entity 'leads' named entity 'discovered' named entity 'potential' named entity 'PHEV' named entity 'restriction' named entity 'specific' named entity 'knockdown' named entity 'alterations' named entity 'constitutively' named entity 'DYSFUNCTION' named entity 'LIFE CYCLE' named entity 'REAL-TIME PCR ' named entity 'VIRUS' named entity 'MICE' named entity 'CONTRIBUTE ' named entity 'A CELL' named entity 'ALTERING' named entity 'EXPRESSION' named entity 'SUPPRESSION' named entity 'WIDESPREAD' named entity 'ABUNDANT' named entity 'REDUCTION' named entity 'QUANTITATIVE REAL-TIME PCR' named entity 'CAUSES' named entity 'PROVIDING' named entity 'EXPLORATION' named entity 'BRAIN' named entity 'LEVELS' named entity 'MODULATING' named entity 'IN VIVO' named entity 'ANTIVIRAL IMMUNE RESPONSE' named entity 'PHEV' named entity 'SINGLE-STRANDED RNA' named entity 'REPORTER' named entity 'PROMOTING' named entity 'DOWNSTREAM' named entity 'MECHANISM' named entity 'PCR' named entity 'PROTEOGLYCAN' named entity 'SYNDECAN 1' named entity 'RNA VIRUS' named entity 'TARGET GENE' named entity 'STRATEGY' named entity 'ALLOWED' named entity 'PATHOGENESIS' named entity 'ACTS' named entity 'GENE REGULATION' named entity 'SDC1' named entity 'WESTERN BLOTTING' named entity 'LUCIFERASE' named entity 'INFECTED HOST' named entity 'MECHANISMS' named entity 'MIR-10A' named entity 'MIRNAS' named entity 'DISEASE' named entity 'ENRICHMENT' named entity 'INFECTION' named entity 'MICRORNA' named entity 'PORCINE HEMAGGLUTINATING ENCEPHALOMYELITIS VIRUS' named entity 'N2A' named entity 'IS A' named entity 'NEGATIVE REGULATION' named entity 'ANALYSIS' named entity 'FINDINGS' named entity 'LEADS' named entity 'FUTURE' named entity 'DEFENSE MECHANISMS' named entity 'INTERACTIONS'

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