About: Identification of one B-cell epitope from NS1 protein of duck Tembusu virus with monoclonal antibodies

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About: Identification of one B-cell epitope from NS1 protein of duck Tembusu virus with monoclonal antibodies Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Identification of one B-cell epitope from NS1 protein of duck Tembusu virus with monoclonal antibodies
Creator	Li, Zhijie Li, Fang Diao, Youxiang Li, Xiuli Lu, Yunjian Ti, Jinfeng
source	PMC
abstract	This study describes the identification of one linear B-cell epitope on TMUV NS1 protein with monoclonal antibody (mAb) 3G2 by indirect enzyme-linked immunosorbent assay (ELISA). In this study, NS1 protein was expressed in prokaryotic expression system and purified. One mAb against NS1 protein was generated from Balb/c mice immunized with recombinant protein NS1. A set of 35 partially-overlapping polypeptides covering the entire NS1 protein was expressed with PGEX-6P-1 vector and screened with mAb 3G2. One polypeptide against the mAb was acquired and identified by indirect ELISA and western-blot. To map the epitope accurately, one or two amino acid residues were removed from the carboxy and amino terminal of polypeptide sequentially. A series of truncated oligopeptides were expressed and purified. The minimal determinant of the linear B cell epitope was recognized and identified with mAb 3G2. The accurate linear B-cell epitope was (269)DEKEIV(274) located in NS1 protein. Furthermore, sequence alignment showed that the epitope was highly conserved and specific among TMUV strains and other flavivirus respectively. The linear B-cell epitope of TMUV NS1 protein could benefit the development of new vaccines and diagnostic assays.
has issue date	2017-07-26(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0181177
bibo:pmid	28746401
has license	cc-by
sha1sum (hex)	32011a9e06d16f2e8bf8f86977aaa8143e9fc692
schema:url	https://doi.org/10.1371/journal.pone.0181177
resource representing a document's title	Identification of one B-cell epitope from NS1 protein of duck Tembusu virus with monoclonal antibodies
has PubMed Central identifier	PMC5528836
has PubMed identifier	28746401
schema:publication	PLoS One
resource representing a document's body	covid:32011a9e06d16f2e8bf8f86977aaa8143e9fc692#body_text
is schema:about of	named entity 'NS1 PROTEIN' named entity 'NS1' named entity 'NS1' named entity 'epitope' named entity 'conserved' named entity 'determinant' named entity 'NS1' named entity 'recombinant protein' named entity 'acquired' named entity 'purified' named entity 'protein' named entity 'epitope' named entity 'NS1 PROTEIN' named entity 'EXPRESSION SYSTEM' named entity 'COVERING' named entity 'PARTIALLY' named entity 'TEMBUSU VIRUS' named entity 'SERIES' named entity 'IDENTIFIED' named entity 'SEQUENCE ALIGNMENT' named entity 'MICE' named entity 'IDENTIFICATION' named entity 'AMINO TERMINAL' named entity 'DIAGNOSTIC' named entity 'DEVELOPMENT' named entity 'IDENTIFIED BY' named entity '3G2' named entity 'LOCATED IN' named entity 'MINIMAL' named entity 'LINEAR' named entity 'BENEFIT' named entity 'MONOCLONAL ANTIBODY' named entity 'DUCK' named entity 'B-CELL EPITOPE' named entity 'PROTEIN ' named entity 'MONOCLONAL ANTIBODIES' named entity 'IDENTIFICATION' named entity 'ENTIRE' named entity 'NS1' named entity 'PROKARYOTIC EXPRESSION' named entity 'HIGHLY' named entity 'NEW' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'ACQUIRED' named entity 'OLIGOPEPTIDES' named entity 'B CELL' named entity 'WESTERN' named entity 'BLOT' named entity 'SPECIFIC' named entity 'AMINO ACID' named entity 'POLYPEPTIDES' named entity 'CARBOXY' named entity 'REMOVED' named entity 'MAB' named entity 'PROTEIN ' named entity 'IMMUNIZED' named entity 'VECTOR' named entity 'STRAINS' named entity 'POLYPEPTIDE' named entity 'CONSERVED' named entity 'OVERLAPPING' named entity 'PURIFIED' named entity 'FLAVIVIRUS' named entity 'MAP' named entity 'VACCINES' named entity 'INDIRECT' named entity 'ELISA' named entity 'ACCURATE' named entity 'STUDY' named entity 'DETERMINANT' named entity 'SET' named entity 'TRUNCATED' named entity 'EPITOPE' named entity 'GENERATED' named entity 'RECOMBINANT PROTEIN' named entity 'B-CELL EPITOPE'

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