About: Exploring optical spectroscopic techniques and nanomaterials for virus detection

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Exploring optical spectroscopic techniques and nanomaterials for virus detection
Creator	Attia, Y Attia, Yasser El-Hussein, A El-Hussein, Ahmed Kasem, Mohamed Lebohang Manoto, Sello Malabi, R Malabi, Rudzani Manoto, S Manoto, Sello Mthunzi-Kufa, P Mthunzi-Kufa, Patience Ombinda-Lemboumba, S Ombinda-Lemboumba, Saturnin Thobakgale, L Thobakgale, Lebogang
Source	Elsevier; PMC
abstract	Viral infections pose significant health challenges globally by affecting millions of people worldwide and consequently resulting in a negative impact on both socioeconomic development and health. Corona virus disease 2019 (COVID-19) is a clear example of how a virus can have a global impact in the society and has demonstrated the limitations of detection and diagnostic capabilities globally. Another virus which has posed serious threats to world health is the human immunodeficiency virus (HIV) which is a lentivirus of the retroviridae family responsible for causing acquired immunodeficiency syndrome (AIDS). Even though there has been a significant progress in the HIV biosensing over the past years, there is still a great need for the development of point of care (POC) biosensors that are affordable, robust, portable, easy to use and sensitive enough to provide accurate results to enable clinical decision making. The aim of this study was to present a proof of concept for detecting HIV-1 pseudoviruses by using anti-HIV1 gp41 antibodies as capturing antibodies. In our study, glass substrates were treated with a uniform layer of silane in order to immobilize HIV gp41 antibodies on their surfaces. Thereafter, the HIV pseudovirus was added to the treated substrates followed by addition of anti-HIV gp41 antibodies conjugated to selenium nanoparticle (SeNPs) and gold nanoclusters (AuNCs). The conjugation of SeNPs and AuNCs to anti-HIV gp41 antibodies was characterized using UV-vis spectroscopy, transmission electron microscopy (TEM) and zeta potential while the surface morphology was characterized by fluorescence microscopy, atomic force microscopy (AFM) and Raman spectroscopy. The UV-vis and zeta potential results showed that there was successful conjugation of SeNPs and AuNCs to anti-HIV gp41 antibodies and fluorescence microscopy showed that antibodies immobilized on glass substrates were able to capture intact HIV pseudoviruses. Furthermore, AFM also confirmed the capturing HIV pseudoviruses and we were able to differentiate between substrates with and without the HIV pseudoviruses. Raman spectroscopy confirmed the presence of biomolecules related to HIV and therefore this system has potential in HIV biosensing applications.
has issue date	2020-08-27(xsd:dateTime)
bibo:doi	10.1016/j.sjbs.2020.08.034
has license	els-covid
sha1sum (hex)	32935a40b63f37564ba8e11791679e6f6a32d890
schema:url	https://doi.org/10.1016/j.sjbs.2020.08.034
resource representing a document's title	Exploring optical spectroscopic techniques and nanomaterials for virus detection
has PubMed Central identifier	PMC7449958
schema:publication	Saudi J Biol Sci
resource representing a document's body	covid:32935a40b63f37564ba8e11791679e6f6a32d890#body_text
is schema:about of	named entity 'socioeconomic development' named entity 'Viral infections' named entity 'virus' named entity 'nanomaterials' named entity 'nanomaterials' named entity 'optical' named entity 'AFFECTING' named entity 'GLOBALLY' named entity 'IMPACT' named entity 'CLEAR' named entity 'NANOMATERIALS' named entity 'PRE' named entity 'globally' named entity 'clear' named entity 'virus' named entity 'optical' named entity 'Viral infections' named entity 'COVID-19' named entity 'optical spectroscopic' named entity 'nanomaterials' named entity 'optical spectroscopic' named entity 'surface area' named entity 'chemical reduction' named entity 'United Kingdom' named entity 'antibody' named entity 'gp41' named entity 'ultrapure water' named entity 'biosensing' named entity 'antibodies' named entity 'biosensor' named entity 'surface chemistry' named entity 'SARS-CoV-2' named entity 'substrates' named entity 'spectroscopy' named entity 'phosphate buffered saline' named entity 'biosensor' named entity 'viral particle' named entity 'biomolecules' named entity 'substrates' named entity 'HIV' named entity 'conjugation' named entity 'optical fibre' named entity 'CCD camera' named entity 'fluorescence' named entity 'laser light' named entity 'glycoprotein gp120' named entity 'TEM' named entity 'biomolecules' named entity 'fluorescence detection' named entity 'absorption spectra' named entity 'Future studies' named entity 'gp41' named entity 'proof of concept' named entity 'quantum dots' named entity 'gold nanoclusters' named entity 'diode laser' named entity 'optical properties' named entity 'proof of concept' named entity 'pseudoviruses' named entity 'Nd:YLF' named entity 'HIV1' named entity 'HIV1' named entity 'conjugation' named entity 'wavelength' named entity '10 nm' named entity 'resonance frequency' named entity 'diagnostic tool' named entity 'substrates'

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