About: Pustulan Activates Chicken Bone Marrow-Derived Dendritic Cells In Vitro and Promotes Ex Vivo CD4(+) T Cell Recall Response to Infectious Bronchitis Virus

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About: Pustulan Activates Chicken Bone Marrow-Derived Dendritic Cells In Vitro and Promotes Ex Vivo CD4(+) T Cell Recall Response to Infectious Bronchitis Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Pustulan Activates Chicken Bone Marrow-Derived Dendritic Cells In Vitro and Promotes Ex Vivo CD4(+) T Cell Recall Response to Infectious Bronchitis Virus
Creator	Christensen, Dennis Dalgaard, Tina Kjaerup, Rikke Guldbrandtsen, Bernt Larsen, Frederik Pitcovski, Jacob
source	Medline; PMC
abstract	Infectious bronchitis virus (IBV) is a highly contagious avian coronavirus. IBV causes substantial worldwide economic losses in the poultry industry. Vaccination with live-attenuated viral vaccines, therefore, are of critical importance. Live-attenuated viral vaccines, however, exhibit the potential for reversion to virulence and recombination with virulent field strains. Therefore, alternatives such as subunit vaccines are needed together with the identification of suitable adjuvants, as subunit vaccines are less immunogenic than live-attenuated vaccines. Several glycan-based adjuvants directly targeting mammalian C-type lectin receptors were assessed in vitro using chicken bone marrow-derived dendritic cells (BM-DCs). The β-1-6-glucan, pustulan, induced an up-regulation of MHC class II (MHCII) cell surface expression, potentiated a strong proinflammatory cytokine response, and increased endocytosis in a cation-dependent manner. Ex vivo co-culture of peripheral blood monocytes from IBV-immunised chickens, and BM-DCs pulsed with pustulan-adjuvanted recombinant IBV N protein (rN), induced a strong recall response. Pustulan-adjuvanted rN induced a significantly higher CD4(+) blast percentage compared to either rN, pustulan or media. However, the CD8(+) and TCRγδ(+) blast percentage were significantly lower with pustulan-adjuvanted rN compared to pustulan or media. Thus, pustulan enhanced the efficacy of MHCII antigen presentation, but apparently not the cross-presentation on MHCI. In conclusion, we found an immunopotentiating effect of pustulan in vitro using chicken BM-DCs. Thus, future in vivo studies might show pustulan as a promising glycan-based adjuvant for use in the poultry industry to contain the spread of coronaviridiae as well as of other avian viral pathogens.
has issue date	2020-05-15(xsd:dateTime)
bibo:doi	10.3390/vaccines8020226
bibo:pmid	32429204
has license	cc-by
sha1sum (hex)	331797dfab2fb2bf5941180434d81bb41f6867a8
schema:url	https://doi.org/10.3390/vaccines8020226
resource representing a document's title	Pustulan Activates Chicken Bone Marrow-Derived Dendritic Cells In Vitro and Promotes Ex Vivo CD4(+) T Cell Recall Response to Infectious Bronchitis Virus
has PubMed Central identifier	PMC7349971
has PubMed identifier	32429204
schema:publication	Vaccines (Basel)
resource representing a document's body	covid:331797dfab2fb2bf5941180434d81bb41f6867a8#body_text
is schema:about of	named entity 'chicken' named entity 'subunit vaccines' named entity 'targeting' named entity 'blast' named entity 'efficacy' named entity 'vaccines' named entity 'assessed' named entity 'alternatives' named entity 'CD8' named entity 'vaccines' named entity 'chickens' named entity 'Dendritic Cells' named entity 'ATTENUATED VACCINES' named entity 'CRITICAL' named entity 'IMMUNOGENIC' named entity 'SPREAD' named entity 'RECOMBINANT' named entity 'PERCENTAGE' named entity 'EITHER' covid:arg/331797dfab2fb2bf5941180434d81bb41f6867a8 named entity 'MHCII' named entity 'coronavirus' named entity 'losses' named entity 'recombination' named entity 'poultry industry' named entity 'reversion' named entity 'virulent' named entity 'Therefore' named entity 'However' named entity 'dendritic cells' named entity 'vaccines' named entity 'manner' named entity 'live-attenuated' named entity 'adjuvanted' named entity 'MHCII' named entity 'viral pathogens' named entity 'live-attenuated' named entity 'co-culture' named entity 'MHCI' named entity 'vaccines' named entity 'glucan' named entity 'C-type lectin receptors' named entity 'identification' named entity 'immunoreceptor tyrosine-based activation motif' named entity 'glucan' named entity 'chitosan' named entity 'glycan' named entity 'IBV' named entity 'IBV' named entity 'biological replicates' named entity 'protein' named entity 'PBMCs' named entity 'murine' named entity 'FMO' named entity 'lichen' named entity 'chitosan' named entity 'adjuvants' named entity 'cation' named entity 'aseptic conditions' named entity 'IBV' named entity 'incubation' named entity 'PBMCs' named entity 'IL-18' named entity 'PerCP' named entity 'IBV' named entity 'cDNA' named entity 'MHC' named entity 'MHC' named entity 'Escherichia coli' named entity 'EDTA' named entity 'recombinant' named entity 'BSA' named entity 'glucan' named entity 'confocal microscopy' named entity 'gel electrophoresis' named entity 'chicken'

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