About: Development of a TaqMan assay for sensitive detection of all pestiviruses infecting cattle, including the emerging HoBi-like strains

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About: Development of a TaqMan assay for sensitive detection of all pestiviruses infecting cattle, including the emerging HoBi-like strains Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development of a TaqMan assay for sensitive detection of all pestiviruses infecting cattle, including the emerging HoBi-like strains
Creator	Buonavoglia, Canio Decaro, Nicola Mari, Viviana Losurdo, Michele Cavaliere, Nicola Colaianni, Maria Lucente, Maria Padalino, Iolanda
source	Elsevier; Medline; PMC
abstract	Abstract A real-time RT-PCR assay based on the TaqMan technology was developed for rapid and sensitive detection of pestiviruses infecting cattle, i.e., bovine viral diarrhea virus (BVDV) 1, BVDV-2, and the emerging HoBi-like pestiviruses. The assay was linear and reproducible, being able to detect as few as 10 copies of viral RNA. By real-time RT-PCR analysis of 986 biological samples collected from cattle herd with clinical signs suggestive of pestivirus infection and from animals recruited in a pestivirus surveillance programme, 165 pestivirus positive samples were detected, including 6 specimens, 2 nasal swabs, and 4 EDTA–blood samples, that tested negative by a gel-based RT-PCR assay targeting the 5′UTR. The developed TaqMan assay represents a new reliable and effective tool for rapid and sensitive diagnosis of infections caused by all pestiviruses circulating in cattle, thus being useful for extensive surveillance programs in geographic areas where HoBi-like pestiviruses are co-circulating with BVDV-1 and BVDV-2.
has issue date	2015-11-30(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2015.08.013
bibo:pmid	26300370
has license	els-covid
sha1sum (hex)	3429fc8d234966efe5bdd556a9cc2bfd0f7ee5e9
schema:url	https://doi.org/10.1016/j.jviromet.2015.08.013
resource representing a document's title	Development of a TaqMan assay for sensitive detection of all pestiviruses infecting cattle, including the emerging HoBi-like strains
has PubMed Central identifier	PMC7113749
has PubMed identifier	26300370
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:3429fc8d234966efe5bdd556a9cc2bfd0f7ee5e9#body_text
is schema:about of	named entity 'linear' named entity 'RNA' named entity 'TaqMan' named entity 'ABLE TO' named entity 'CATTLE' covid:arg/3429fc8d234966efe5bdd556a9cc2bfd0f7ee5e9 named entity 'TAQMAN' named entity 'LIKE' named entity 'CIRCULATING' named entity 'PROGRAMS' named entity 'ASSAY' named entity 'LINEAR' named entity 'TESTED' named entity 'HERD' named entity 'PESTIVIRUS' named entity 'ANIMALS' named entity 'TO DETECT' named entity 'PESTIVIRUS INFECTION' named entity 'PESTIVIRUSES' named entity 'COLLECTED' named entity 'TAQMAN' named entity 'TARGETING' named entity 'UTR' named entity 'SURVEILLANCE' named entity 'EDTA' named entity 'INFECTIONS' named entity 'CATTLE' named entity 'RT-PCR ASSAY' named entity 'GEL' named entity 'PCR ANALYSIS' named entity 'INFECTING' named entity 'SPECIMENS' named entity 'DIAGNOSIS' named entity 'SENSITIVE' named entity 'ASSAY' named entity 'INFECTING' named entity 'DETECTION' named entity 'RAPID' named entity 'BVDV-2' named entity 'PESTIVIRUSES' named entity 'DETECTION' named entity 'STRAINS' named entity 'DEVELOPMENT' named entity 'INCLUDING' named entity 'BASED' named entity 'NEW' named entity 'BLOOD SAMPLES' named entity 'RELIABLE' named entity 'BOVINE VIRAL DIARRHEA VIRUS' named entity 'SUGGESTIVE OF' named entity 'CAUSED BY' named entity 'SENSITIVE' named entity '165' named entity 'REAL-TIME RT-PCR' named entity 'COPIES' named entity 'NASAL SWABS' named entity 'POSITIVE' named entity 'GEOGRAPHIC AREAS' named entity 'EFFECTIVE' named entity 'PROGRAMME' named entity 'LIKE' named entity 'BIOLOGICAL' named entity 'TECHNOLOGY' named entity 'TOOL' named entity 'NEGATIVE' named entity 'SAMPLES' named entity 'DETECTED' named entity 'EXTENSIVE' named entity 'WHERE' named entity 'BEING' named entity 'VIRAL RNA' named entity 'INCLUDING' named entity 'USEFUL' named entity 'based'

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