About: Bacterial pathogens were detected from human exhaled breath using a novel protocol

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Bacterial pathogens were detected from human exhaled breath using a novel protocol
Creator	Yao, Maosheng Li, Xiaoguang Chen, Haoxuan Zheng, Yunhao
source	Elsevier; Medline; PMC
abstract	Abstract It is generally believed that influenza outbreak is associated with breath-borne transmission of viruses, however relevant evidence is little for that of respiratory bacterial infections. On another front, point-of-care infection diagnostic methods at the bedside are significantly lacking. Here, we used a newly developed protocol of integrating an exhaled breath condensate (EBC) collection device (PKU BioScreen) and Loop Mediated Isothermal Amplification (LAMP) to investigate what bacterial pathogens can be directly exhaled out from humans. Exhaled breath condensates were collected from human subjects with respiratory infection symptoms at Peking University 3rd hospital using the BioScreen. The screened bacterial pathogens included Streptococcus pneumoniae, Staphylococcus aureus, Methicillin-resistant Stphylococcus aureus (MRSA), Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia, Haemophilus influenzae, Legionella pneumophila, Mycoplasma Pneumonia, Chlamydia pneumonia, and Mycobacterium tuberculosis. The results were further compared and validated using throat swabs from the same patients by a PCR method. Here, human bacterial pathogens such as H. influenzae, P. aeruginosa, E. coli, S. aureus and MRSA were detected in exhaled breath using the developed protocol that integrates the EBC collection and LAMP. For the patients recruited from the hospital, seven types of pathogens were detected from 36.5% of them, and for the remaining subjects none of those screened bacterial pathogens was detected. Importantly, some super resistant bacteria such as MRSA were detected from the exhaled breath, suggesting that breathing might be also an important bacterial transmission route. Results from throat swabs showed that 36.2% of the subjects were found to be infected with H. influenzae, P. aeruginosa, E. coli, S. maltophilia, S. aureus and MRSA. For the EBC samples, 33.3% were found to be infected with MRSA, E. coli and P. aeruginosa. Depending on the initial pathogen load in the sample, the entire protocol (EBC-LAMP) only takes 20–60min to complete for a respiratory infection diagnosis. For different detection methods and pathogens, the agreements between the EBC and throat swabs from the same patients were found to range from 35% to 65%. Here, we have detected several bacterial pathogens including MRSA from exhaled breath, and the developed protocol could be very useful for the bedside pathogen screening particularly in remote areas where resources are significantly limited or prohibited.
has issue date	2018-03-31(xsd:dateTime)
bibo:doi	10.1016/j.jaerosci.2017.12.009
bibo:pmid	32226119
has license	els-covid
sha1sum (hex)	35acfbce6a0ccde0195293222f6bbf2dc9877dd6
schema:url	https://doi.org/10.1016/j.jaerosci.2017.12.009
resource representing a document's title	Bacterial pathogens were detected from human exhaled breath using a novel protocol
has PubMed Central identifier	PMC7094568
has PubMed identifier	32226119
schema:publication	Journal of Aerosol Science
resource representing a document's body	covid:35acfbce6a0ccde0195293222f6bbf2dc9877dd6#body_text
is schema:about of	named entity 'influenza' named entity 'symptoms' named entity 'Mycobacterium tuberculosis' named entity 'humans' named entity 'evidence' named entity 'developed' named entity 'investigate' named entity 'EBC' named entity 'Mycoplasma' named entity 'compared' named entity 'generally' named entity 'outbreak' named entity 'bacterial pathogens' named entity 'subjects' named entity 'Chlamydia' named entity 'condensate' named entity 'Methicillin-resistant' named entity 'respiratory infection' named entity 'PCR' named entity 'human subjects' named entity 'infection' named entity 'pneumonia' named entity 'bacterial pathogens' named entity 'Peking University' named entity 'Stenotrophomonas maltophilia' named entity 'exhaled breath condensate' named entity 'Mycobacterium tuberculosis' named entity 'exhaled breath' named entity 'throat swab' named entity 'qPCR' named entity 'MRSA' named entity 'pathogens' named entity 'pathogen' named entity 'M. pneumoniae' named entity 'H. influenzae' named entity 'WBC' named entity 'Legionella pneumophila' named entity 'bioaerosol' named entity 'fluorescent dye' named entity 'detection limits' named entity 'ultraviolet radiation' named entity 'virus' named entity 'White blood cells' named entity 'Bacteria' named entity 'clinical presentation' named entity 'cross contamination' named entity 'E. coli' named entity 'Positive and negative controls' named entity 'MgSO' named entity 'pathogen' named entity 'gold standard' named entity 'WHO' named entity 'flu symptoms' named entity 'Japan' named entity 'white blood cell' named entity 'H. influenzae' named entity 'DI water' named entity 'throat swab' named entity 'pathogens' named entity 'agarose gel' named entity 'PCR' named entity 'NH 4' named entity 'Tween 20' named entity 'S. aureus' named entity 'H. influenzae' named entity 'pathogens' named entity 'pathogens' named entity 'data analysis' named entity 'minor role' named entity 'H. influenza' named entity 'ambient air' named entity 'electrophoresis' named entity 'RNA' named entity 'throat swab' named entity 'Eppendorf' named entity 'H. influenzae' named entity 'aerosol' named entity 'hydrophobic'

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