About: Diversity and quantity of ammonia-oxidizing Archaea and Bacteria in sediment of the Pearl River Estuary, China

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About: Diversity and quantity of ammonia-oxidizing Archaea and Bacteria in sediment of the Pearl River Estuary, China Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Diversity and quantity of ammonia-oxidizing Archaea and Bacteria in sediment of the Pearl River Estuary, China
Creator	Jin, Tao Zhang, Tong Ye, Lin Qian, Pei Him, Wong Lee, Yue On, On
Source	PMC
abstract	The diversity and abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in the sediment of the Pearl River Estuary were investigated by cloning and quantitative real-time polymerase chain reaction (qPCR). From one sediment sample S16, 36 AOA OTUs (3% cutoff) were obtained from three clone libraries constructed using three primer sets for amoA gene. Among the 36 OTUs, six were shared by all three clone libraries, two appeared in two clone libraries, and the other 28 were only recovered in one of the libraries. For AOB, only seven OTUs (based on 16S rRNA gene) and eight OTUs (based on amoA gene) were obtained, showing lower diversity than AOA. The qPCR results revealed that AOA amoA gene copy numbers ranged from 9.6 × 10(6) to 5.1 × 10(7) copies per gram of sediment and AOB amoA gene ranged from 9.5 × 10(4) to 6.2 × 10(5) copies per gram of sediment, indicating that the dominant ammonia-oxidizing microorganisms in the sediment of the Pearl River Estuary were AOA. The terminal restriction fragment length polymorphism results showed that the relative abundance of AOB species in the sediment samples of different salinity were significantly different, indicating that salinity might be a key factor shaping the AOB community composition. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00253-011-3107-8) contains supplementary material, which is available to authorized users.
has issue date	2011-02-01(xsd:dateTime)
bibo:doi	10.1007/s00253-011-3107-8
bibo:pmid	21286709
has license	no-cc
sha1sum (hex)	3afdbd963a3949ef7e8bbcc54f3acd9179bbcdaa
schema:url	https://doi.org/10.1007/s00253-011-3107-8
resource representing a document's title	Diversity and quantity of ammonia-oxidizing Archaea and Bacteria in sediment of the Pearl River Estuary, China
has PubMed Central identifier	PMC3076564
has PubMed identifier	21286709
schema:publication	Appl Microbiol Biotechnol
resource representing a document's body	covid:3afdbd963a3949ef7e8bbcc54f3acd9179bbcdaa#body_text
is schema:about of	named entity 'abundance' named entity 'salinity' named entity 'clone' named entity 'factor' named entity 'gene' named entity 'AOA' named entity 'polymorphism' named entity 'OTUs' named entity 'Pearl River' named entity 'INVESTIGATED' named entity '9710' named entity 'OBTAINED' named entity 'AMMONIA' named entity 'S16' named entity 'INDICATING' named entity 'BACTERIA ' named entity 'SEDIMENT' named entity 'libraries' named entity 'libraries' named entity 'cloning' named entity 'Estuary' named entity 'libraries' named entity 'AOB' named entity 'libraries' named entity 'AOB' named entity 'qPCR' named entity 'polymerase' named entity 'gene' named entity 'S16' named entity 'OTUs' named entity 'amoA' named entity 'sediment' named entity 'qPCR' named entity 'sediment' named entity 'AOB' named entity 'AOA' named entity '16S rRNA gene' named entity 'Pearl River Estuary' named entity 'gene' named entity 'Archaea and Bacteria' named entity 'investigated' named entity 'sets' named entity 'S19' named entity 'gene copy numbers' named entity 'DNA polymerase' named entity '16S rRNA' named entity 'Jukes-Cantor' named entity 'genus' named entity 'qPCR' named entity 'hydroxylamine' named entity 'Archaeal' named entity 'AOB' named entity '16S rRNA' named entity 'estuarine' named entity '16S rRNA gene' named entity 'S16' named entity 'AOB' named entity 'Biotechnology' named entity 'Southern China' named entity 'PCR' named entity 'AOB' named entity 'sediment' named entity 'quantitative PCR' named entity '5.6' named entity 'Crenarchaeota' named entity 'Pearl River Estuary' named entity 'AOA' named entity 'qPCR' named entity 'denitrification' named entity 'gene' named entity 'Phylogenetic analysis' named entity 'estuaries' named entity 'nitrification' named entity 'AOB' named entity 'clone'

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