About: Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo

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About: Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo
Creator	Li, Min Chen, Xiao-Yue Cheng, An-Chun Guo, Yu-Fei Li, Chuan-Feng Pan, Kang-Cheng Wang, Ming-Shu Yang, Jin-Long Zhu, De-Kang
Source	Medline; PMC
abstract	BACKGROUND: Goose parvovirus (GPV) is a Dependovirus associated with latent infection and mortality in geese. Currently, it severely affects geese production worldwide. The objective of this study was to develop a fluorescent quantitative real-time polymerase chain reaction (PCR) (FQ-PCR) assay for fast and accurate quantification of GPV DNA in infected goslings, which can aid in the understanding of the regular distribution pattern and the nosogenesis of GPV in vivo. RESULTS: The detection limit of the assay was 2.8 × 10(1 )standard DNA copies, with a sensitivity of 3 logs higher than that of the conventional gel-based PCR assay targeting the same gene. The real-time PCR was reproducible, as shown by satisfactory low intraassay and interassay coefficients of variation. CONCLUSION: The high sensitivity, specificity, simplicity, and reproducibility of the GPV fluorogenic PCR assay, combined with a high throughput, make this method suitable for a broad spectrum of GPV etiology-related applications.
has issue date	2009-09-15(xsd:dateTime)
bibo:doi	10.1186/1743-422x-6-142
bibo:pmid	19754946
has license	cc-by
sha1sum (hex)	3bac7494aeafbb2f2cff25690dac67e99c120029
schema:url	https://doi.org/10.1186/1743-422x-6-142
resource representing a document's title	Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo
has PubMed Central identifier	PMC2751755
has PubMed identifier	19754946
schema:publication	Virol J
resource representing a document's body	covid:3bac7494aeafbb2f2cff25690dac67e99c120029#body_text
is schema:about of	named entity 'FLUORESCENT' named entity 'distribution' named entity 'fast' named entity 'Goose' named entity 'GEESE' named entity 'WORLDWIDE' named entity 'OBJECTIVE' named entity 'VIROLOGY' covid:arg/3bac7494aeafbb2f2cff25690dac67e99c120029 named entity 'CURRENTLY' named entity 'BACKGROUND' named entity 'IN VIVO' named entity 'FAST' named entity 'REAL-TIME POLYMERASE CHAIN REACTION ASSAY' named entity 'DETECTION' named entity 'QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION' named entity 'IN VIVO' named entity 'DEVELOPMENT' named entity 'GOOSE PARVOVIRUS' named entity 'ASSOCIATED WITH' named entity 'REGULAR' named entity 'DEPENDOVIRUS' named entity 'SEVERELY' named entity 'IS A' named entity 'STUDY' named entity 'LATENT INFECTION' named entity 'UNDERSTANDING' named entity 'PCR' named entity 'FLUORESCENT' named entity 'MORTALITY' named entity 'DNA' named entity 'DISTRIBUTION PATTERN' named entity 'AID' named entity 'ASSAY' named entity 'GPV' named entity 'JOURNAL ' named entity 'INFECTED' named entity 'ACCURATE' named entity 'QUANTIFICATION' named entity 'QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION' named entity 'GOOSE PARVOVIRUS' named entity 'PRODUCTION' named entity 'production' named entity 'assay' named entity 'distribution pattern' named entity 'real-time polymerase chain reaction' named entity '5.0' named entity 'PCR' named entity 'PCR' named entity 'PCR' named entity 'PCR' named entity 'PCR' named entity 'real-time PCR' named entity 'thymus' named entity 'gene' named entity 'correlation coefficient' named entity 'PCR' named entity 'Sichuan Agricultural University' named entity 'recombinant' named entity 'mmol/L' named entity 'cloned' named entity 'dynamic range' named entity 'Sichuan' named entity 'genus' named entity 'HPLC' named entity 'GPV' named entity 'PCR' named entity 'tissue sample' named entity 'pathogenesis' named entity 'infection' named entity 'PCR' named entity 'infection' named entity 'primer'

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