About: Modulatory effect of rRNA synthesis and ppUL83 nucleolar compartmentalization on human cytomegalovirus gene expression in vitro

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About: Modulatory effect of rRNA synthesis and ppUL83 nucleolar compartmentalization on human cytomegalovirus gene expression in vitro Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Modulatory effect of rRNA synthesis and ppUL83 nucleolar compartmentalization on human cytomegalovirus gene expression in vitro
Creator	Chezzi, Carlo De Conto, Flora Ferraglia, Francesca Arcangeletti, Maria-Cristina Covan, Silvia Dettori, Giuseppe Gatti, Rita Motta, Federica Orlandini, Guido Razin, Sergey Rodighiero, Isabella
topic	covid:3cf6714ce7d8f2e7d22dd9a62a1d4986710a3896#this
source	Medline; PMC
abstract	The nucleolus is a nuclear domain involved in the biogenesis of ribosomes, as well as in many other important cellular regulatory activities, such as cell cycle control and mRNA processing. Many viruses, including herpesviruses, are known to exploit the nucleolar compartment during their replication cycle. In a previous study, we demonstrated the preferential targeting and accumulation of the human cytomegalovirus (HCMV) UL83 phosphoprotein (pp65) to the nucleolar compartment and, in particular, to the nucleolar matrix of lytically infected fibroblasts; such targeting was already evident at very early times after infection. Here we have investigated the possible effects of rRNA synthesis inhibition upon the development of HCMV lytic infection, by using either actinomycin D or cisplatin at low concentrations, that are known to selectively inhibit RNA polymerase I activity, whilst leaving RNA polymerase II function unaffected. Following the inhibition of rRNA synthesis by either of the agents used, we observed a significant redistribution of nucleolar proteins within the nucleoplasm and a simultaneous depletion of viral pp65 from the nucleolus; this effect was highly evident in both unextracted cells and in nuclear matrices in situ. Of particular interest, even a brief suppression of rRNA synthesis resulted in a very strong inhibition of the progression of HCMV infection, as was concluded from the absence of accumulation of HCMV major immediate‐early proteins within the nucleus of infected cells. These data suggest that a functional relationship might exist between rRNA synthesis, pp65 localization to the nucleolar matrix and the normal development of HCMV lytic infection. J. Cell. Biochem. 108: 415–423, 2009. © 2009 Wiley‐Liss, Inc.
has issue date	2009-07-07(xsd:dateTime)
bibo:doi	10.1002/jcb.22268
bibo:pmid	19585527
has license	no-cc
sha1sum (hex)	3cf6714ce7d8f2e7d22dd9a62a1d4986710a3896
schema:url	https://doi.org/10.1002/jcb.22268
resource representing a document's title	Modulatory effect of rRNA synthesis and ppUL83 nucleolar compartmentalization on human cytomegalovirus gene expression in vitro
has PubMed Central identifier	PMC7167110
has PubMed identifier	19585527
schema:publication	J Cell Biochem
resource representing a document's body	covid:3cf6714ce7d8f2e7d22dd9a62a1d4986710a3896#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/3cf6714ce7d8f2e7d22dd9a62a1d4986710a3896
is schema:about of	named entity 'matrix' named entity 'development' named entity 'cisplatin' named entity 'nucleolar' named entity 'nucleolus' named entity 'selectively' named entity 'These' named entity 'viruses' named entity 'CELLS' named entity 'EITHER' named entity 'PARTICULAR' named entity 'COMPARTMENT' named entity 'STUDY' named entity 'FUNCTION' named entity 'BIOGENESIS' named entity 'EXIST' named entity 'KNOWN' named entity 'NUCLEOLAR' named entity 'MAJOR' named entity 'INTEREST' named entity 'EFFECTS' named entity 'LOW' named entity 'HERPESVIRUSES' named entity 'PREVIOUS' named entity 'INVESTIGATED' covid:arg/3cf6714ce7d8f2e7d22dd9a62a1d4986710a3896 named entity 'accumulation' named entity 'processing' named entity 'infected' named entity 'HCMV' named entity 'investigated' named entity 'observed' named entity 'inhibit' named entity 'study' named entity 'leaving' named entity 'polymerase' named entity 'accumulation' named entity 'evident' named entity 'HCMV' named entity 'infection' named entity 'cell cycle control' named entity 'RNA polymerase II' named entity 'HCMV' named entity 'biogenesis' named entity 'Human Cytomegalovirus' named entity 'rRNA' named entity 'actin' named entity 'RNAs' named entity 'gene' named entity 'primary antibodies' named entity 'quantitative PCR' named entity 'non-essential' named entity 'internal transcribed spacer' named entity 'denaturing' named entity 'nuclei' named entity 'cDNA' named entity 'exon' named entity 'signaling pathways' named entity 'pre-mRNA' named entity 'viral antigens' named entity 'immediate-early' named entity 'serum' named entity 'kinetics' named entity 'ribosomal proteins' named entity 'DMSO' named entity 'infection' named entity 'cell cycle progression' named entity 'RNA synthesis' named entity 'transcription inhibitors'

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