About: Characterization of germline antibody libraries from human umbilical cord blood and selection of monoclonal antibodies to viral envelope glycoproteins: implications for mechanisms of immune evasion and design of vaccine immunogens

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About: Characterization of germline antibody libraries from human umbilical cord blood and selection of monoclonal antibodies to viral envelope glycoproteins: implications for mechanisms of immune evasion and design of vaccine immunogens Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Characterization of germline antibody libraries from human umbilical cord blood and selection of monoclonal antibodies to viral envelope glycoproteins: implications for mechanisms of immune evasion and design of vaccine immunogens
Creator	Prabakaran, Ponraj Feng, Yang Chen, Weizao Streaker, Emily Biophys, Biochem Commun, Res Dimitrov, Dimiter Russ, Daniel
topic	covid:3e576312dc83755e3c8ba63e6205553c0aae93d0#this
source	Elsevier; Medline; PMC
abstract	We have previously observed that all known HIV-1 broadly neutralizing antibodies (bnAbs) are highly divergent from germline antibodies in contrast to bnAbs against Hendra virus, Nipah virus and SARS coronavirus (SARS CoV). We have hypothesized that because the germline antibodies are so different from the mature HIV-1-specific bnAbs they may not bind the epitopes of the mature antibodies and provided the first evidence to support this hypothesis by using individual putative germline-like predecessor antibodies. To further validate the hypothesis and understand initial immune responses to different viruses, two phage-displayed human cord blood-derived IgM libraries were constructed which contained mostly germline antibodies or antibodies with very low level of somatic hypermutations. They were panned against different HIV-1 envelope glycoproteins (Envs), SARS CoV protein receptor-binding domain (RBD), and soluble Hendra virus G protein (sG). Despite a high sequence and combinatorial diversity observed in the cord blood-derived IgM antibody repertoire, no enrichment for binders of Envs was observed in contrast to considerable specific enrichments produced with panning against RBD and sG; one of the selected monoclonal antibodies (against the RBD) was of high (nM) affinity with only few somatic mutations. These results further support and expand our initial hypothesis for fundamental differences in immune responses leading to elicitation of bnAbs against HIV-1 compared to SARS CoV and Hendra virus. HIV-1 uses a strategy to minimize or eliminate strong binding of germline antibodies to its Env; in contrast, SARS CoV and Hendra virus, and perhaps other viruses causing acute infections, can bind germline antibody or minimally somatically mutated antibodies with relatively high affinity which could be one of the reasons for the success of sG and RBD as vaccine immunogens.
has issue date	2012-01-01(xsd:dateTime)
bibo:doi	10.1016/j.bbrc.2011.12.089
bibo:pmid	22226962
has license	green-oa
sha1sum (hex)	3e576312dc83755e3c8ba63e6205553c0aae93d0
schema:url	https://doi.org/10.1016/j.bbrc.2011.12.089
resource representing a document's title	Characterization of germline antibody libraries from human umbilical cord blood and selection of monoclonal antibodies to viral envelope glycoproteins: implications for mechanisms of immune evasion and design of vaccine immunogens
has PubMed Central identifier	PMC3268823
has PubMed identifier	22226962
schema:publication	Biochemical and Biophysical Research Communications
resource representing a document's body	covid:3e576312dc83755e3c8ba63e6205553c0aae93d0#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/3e576312dc83755e3c8ba63e6205553c0aae93d0
is schema:about of	named entity 'MANUSCRIPT' named entity 'PUBLIC' named entity 'somatic mutations' named entity 'soluble' named entity 'leading' named entity 'level' named entity 'antibody' named entity 'antibodies' named entity 'antibodies' named entity 'expand' named entity 'reasons' named entity 'affinity' named entity 'antibody' named entity 'immune' named entity 'umbilical cord blood' named entity 'NIH' named entity 'ONE OF' named entity 'PHAGE' named entity 'HYPOTHESIS' named entity 'KNOWN' named entity 'VERY LOW LEVEL' named entity 'CONTAINED' named entity 'ENVELOPE' named entity 'EVIDENCE' named entity 'DIVERSITY' named entity 'ITS' named entity 'MONOCLONAL ANTIBODIES' named entity 'ANTIBODY' named entity 'SELECTED' named entity 'UNDERSTAND' named entity 'DOMAIN' named entity 'HIGH' named entity 'IGM ANTIBODY' named entity 'AFFINITY' named entity 'MONOCLONAL ANTIBODIES' named entity 'VACCINE' named entity 'GLYCOPROTEINS' named entity 'HUMAN' named entity 'GERMLINE' named entity 'SARS' named entity 'HIV-1' named entity 'THESE' named entity 'CAUSING' named entity 'NIPAH VIRUS' named entity 'EXPAND' named entity 'GERMLINE' named entity 'MECHANISMS' named entity 'CHARACTERIZATION' named entity 'DESIGN' named entity 'ACCESS' named entity 'IMMUNE EVASION' named entity 'NIH' named entity 'AUTHOR' named entity 'SELECTION' named entity 'UMBILICAL CORD BLOOD' named entity 'VIRAL ENVELOPE' named entity 'LIBRARIES' named entity 'ANTIBODY' named entity 'IMMUNOGENS' named entity 'SOLUBLE' named entity 'COMPARED' named entity 'DIFFERENT' named entity 'STRATEGY' named entity 'SUCCESS' named entity 'MATURE' named entity 'HAVE' named entity 'REASONS' named entity 'DISPLAYED' named entity 'ENRICHMENTS' named entity 'CONTRAST' named entity 'RBD' named entity 'SOMATIC'

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