About: Microarray for Identification of the Chiropteran Host Species of Rabies Virus in Canada

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Microarray for Identification of the Chiropteran Host Species of Rabies Virus in Canada Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Microarray for Identification of the Chiropteran Host Species of Rabies Virus in Canada
Creator	Ambagala, Aruna Erickson, Anthony Fisher, Mathew Furukawa-Stoffer, Tara Lung, Oliver Nadin-Davis, Susan Knowles, M
Source	Medline; PMC
abstract	Species identification through genetic barcoding can augment traditional taxonomic methods, which rely on morphological features of the specimen. Such approaches are especially valuable when specimens are in poor condition or comprise very limited material, a situation that often applies to chiropteran (bat) specimens submitted to the Canadian Food Inspection Agency for rabies diagnosis. Coupled with phenotypic plasticity of many species and inconclusive taxonomic keys, species identification using only morphological traits can be challenging. In this study, a microarray assay with associated PCR of the mitochondrial cytochrome c oxidase subunit I (COI) gene was developed for differentiation of 14 bat species submitted to the Canadian Food Inspection Agency from 1985–2012 for rabies diagnosis. The assay was validated with a reference collection of DNA from 153 field samples, all of which had been barcoded previously. The COI gene from 152 samples which included multiple specimens of each target species were successfully amplified by PCR and accurately identified by the microarray. One sample that was severely decomposed failed to amplify with PCR primers developed in this study, but amplified weakly after switching to alternate primers and was accurately typed by the microarray. Thus, the chiropteran microarray was able to accurately differentiate between the 14 species of Canadian bats targeted. This PCR and microarray assay would allow unequivocal identification to species of most, if not all, bat specimens submitted for rabies diagnosis in Canada.
has issue date	2013-05-31(xsd:dateTime)
bibo:doi	10.3390/microarrays2020153
bibo:pmid	27605186
has license	cc-by
sha1sum (hex)	3e72fd7554ad263eefddd4a0c6ee06afea606a44
schema:url	https://doi.org/10.3390/microarrays2020153
resource representing a document's title	Microarray for Identification of the Chiropteran Host Species of Rabies Virus in Canada
has PubMed Central identifier	PMC5003475
has PubMed identifier	27605186
schema:publication	Microarrays (Basel)
resource representing a document's body	covid:3e72fd7554ad263eefddd4a0c6ee06afea606a44#body_text
is schema:about of	named entity 'augment' named entity 'chiropteran' named entity 'OPEN' named entity 'target' named entity 'rabies' named entity 'barcoded' named entity 'Such' named entity 'ABLE TO' named entity 'OPEN' covid:arg/3e72fd7554ad263eefddd4a0c6ee06afea606a44 named entity 'INCONCLUSIVE' named entity 'SPECIES' named entity 'APPLIES TO' named entity 'RABIES' named entity 'CANADIAN' named entity 'FAILED' named entity 'SEVERELY' named entity 'SUBMITTED' named entity 'PREVIOUSLY' named entity 'AMPLIFIED' named entity 'DIAGNOSIS' named entity 'FEATURES' named entity 'SWITCHING' named entity 'PCR PRIMERS' named entity 'ACCESS' named entity 'MICROARRAY' named entity 'IDENTIFICATION' named entity 'TRADITIONAL' named entity 'MULTIPLE' named entity 'CONDITION' named entity 'PRIMERS' named entity 'PHENOTYPIC PLASTICITY' named entity 'SAMPLES' named entity 'MICROARRAY ASSAY' named entity 'INCLUDED' named entity 'CANADA' named entity 'AMPLIFY' named entity 'SPECIES IDENTIFICATION' named entity 'TRAITS' named entity 'MORPHOLOGICAL' named entity 'STUDY' named entity '152' named entity 'IDENTIFIED BY' named entity 'GENE' named entity 'SAMPLE' named entity 'BUT' named entity 'USING' named entity 'MATERIAL' named entity 'AUGMENT' named entity 'ASSAY' named entity 'POOR' named entity 'BARCODING' named entity 'AGENCY' named entity 'SPECIMEN' named entity 'KEYS' named entity 'COUPLED' named entity 'GENETIC' named entity 'APPROACHES' named entity 'COI GENE' named entity 'DIFFERENTIATE' named entity 'TARGET' named entity 'REFERENCE' named entity 'FIELD' named entity 'VERY LIMITED' named entity 'CYTOCHROME C OXIDASE SUBUNIT I' named entity 'ALTERNATE' named entity 'WITH ASSOCIATED' named entity 'MICROARRAY' named entity 'DIFFERENTIATION' named entity 'FOOD INSPECTION' named entity 'DNA' named entity 'PCR' named entity 'COLLECTION' named entity 'BAT' named entity 'HOST SPECIES'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software