About: Differential tropism of human herpesvirus 6 (HHV-6) variants and induction of latency by HHV-6A in oligodendrocytes

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About: Differential tropism of human herpesvirus 6 (HHV-6) variants and induction of latency by HHV-6A in oligodendrocytes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Differential tropism of human herpesvirus 6 (HHV-6) variants and induction of latency by HHV-6A in oligodendrocytes
Creator	Fotheringham, Julie Jacobson, Steven Yao, Karen Ahlqvist, Jenny Akhyani, Nahid Fogdell-Hahn, Anna
Source	PMC
abstract	Human herpesvirus 6 (HHV-6) is a ubiquitous β-herpesvirus associated with a number of clinical disorders. Two closely but biologically distinct variants have been described. HHV-6 variant B causes the common childhood disease exhanthem subitum, and although the pathologic characteristics for HHV-6 variant A are less well defined, HHV-6A has been suggested to be more neurotropic. We studied the effect of both HHV-6 variants in an oligodendrocyte cell line (MO3.13). Infection of M03.13 was monitored by cytopathic effect (CPE), quantitative TaqMan PCR for viral DNA in cells and supernatant, reverse transcriptase-polymerase chain reaction (RT-PCR) to detect viral RNA, and indirect immunofluorescence (IFA) to detect viral protein expression. HHV-6A infection induced significantly more CPE than infection with HHV-6B. HHV-6B induced an abortive infection associated with a decrease of the initial viral DNA load over time, early RNA expression, and no expression of viral antigen. In contrast, infection with HHV-6A DNA persisted in cells for at least 62 days. During the acute phase of infection with HHV-6A, intracellular and extracellular viral load increased and cells expressed the viral protein IE-2 and gp116/54/64. No HHV-6A RNA or protein was expressed after 30 days post infection, suggesting that HHV-6A formed a latent infection. These studies provide in vitro support to the hypothesis that HHV-6 can actively infect oligodendrocytes. Our results suggest that HHV-6A and HHV-6B have different tropism in MO3.13 cells and that an initially active HHV-6A infection can develop latency. Differences between HHV-6A and -6B infection in different neural cell types may be associated with different neurological diseases.
has issue date	2005-01-01(xsd:dateTime)
bibo:doi	10.1080/13550280591002379
bibo:pmid	16162481
has license	no-cc
sha1sum (hex)	3ff48e99f52808b9176b3de727b91ce40d1aefdb
schema:url	https://doi.org/10.1080/13550280591002379
resource representing a document's title	Differential tropism of human herpesvirus 6 (HHV-6) variants and induction of latency by HHV-6A in oligodendrocytes
has PubMed Central identifier	PMC7095087
has PubMed identifier	16162481
schema:publication	J Neurovirol
resource representing a document's body	covid:3ff48e99f52808b9176b3de727b91ce40d1aefdb#body_text
is schema:about of	named entity 'HUMAN HERPESVIRUS 6' named entity 'OLIGODENDROCYTES' named entity 'PCR' named entity 'viral' named entity 'provide' named entity 'viral' named entity 'time' named entity 'VARIANTS' named entity 'HHV' named entity 'LOAD' named entity 'INFECT' named entity 'VIRAL PROTEIN' named entity 'JOURNAL ' named entity 'TO DETECT' named entity 'NUMBER OF' named entity 'PROTEIN EXPRESSION' named entity 'VARIANT' named entity 'RESULTS' named entity 'DISEASES' named entity 'DISTINCT' named entity 'RNA' named entity 'VIRAL DNA' named entity 'VARIANTS' named entity 'HYPOTHESIS' named entity 'CONTRAST' named entity 'THESE' named entity 'WELL DEFINED' named entity 'M03' named entity 'HHV-6' named entity 'INTRACELLULAR' named entity 'DISORDERS' named entity 'PROVIDE' named entity 'CPE' named entity 'INFECTION INDUCED' named entity 'LATENCY' named entity 'EXTRACELLULAR' named entity 'INDUCTION' named entity 'TROPISM' named entity 'CELLS' named entity 'ACTIVE' named entity 'SUPERNATANT' named entity 'DECREASE' named entity 'PROTEIN ' named entity 'OLIGODENDROCYTE' named entity 'PCR' named entity 'OUR' named entity 'HUMAN HERPESVIRUS 6' named entity 'CHILDHOOD' named entity 'MAY BE' named entity 'DIFFERENT' named entity 'POST' named entity 'ACUTE PHASE' named entity '384' named entity 'ABORTIVE INFECTION' named entity 'VIRAL LOAD INCREASED' named entity 'MO3' named entity 'NEURAL CELL' named entity 'DISEASE' named entity 'VIRAL RNA' named entity 'CELL LINE' named entity '385' named entity 'TROPISM' named entity 'HERPESVIRUS' named entity 'BIOLOGICALLY' named entity 'STUDIES' named entity 'BUT' named entity 'IN VITRO' named entity 'REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION' named entity 'EXPRESSED' named entity 'HHV' named entity 'CELL TYPES' named entity 'ASSOCIATED WITH' named entity 'INITIAL' named entity 'CYTOPATHIC EFFECT' named entity 'COMMON' named entity 'UBIQUITOUS'

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