About: Rapid diagnosis of avian infectious bronchitis virus by the polymerase chain reaction

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About: Rapid diagnosis of avian infectious bronchitis virus by the polymerase chain reaction Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid diagnosis of avian infectious bronchitis virus by the polymerase chain reaction
Creator	Falcone, Emiliana Sili, Amalia Tollis, Maria Trani, Livia D'amore, Emanuela
Source	Elsevier; Medline; PMC
abstract	Abstract A simple, sensitive and specific polymerase chain reaction (PCR) procedure was developed in order to detect infectious bronchitis virus (IBV) directly in tissue samples. Viral RNA was extracted from allantoic fluids and cell cultures infected experimentally with different strains of IBV and from tissues of naturally infected birds. Viral RNA was then amplified and identified by a nested RT-PCR assay using two sets of primers flanking a well-conserved region of the nucleocapsid gene. The selected IBV nucleocapsid sequence was detected successfully by simple direct electrophoresis of amplified material.
has issue date	1997-03-31(xsd:dateTime)
bibo:doi	10.1016/s0166-0934(96)02151-9
bibo:pmid	9079758
has license	els-covid
sha1sum (hex)	441459463bb273ccd5b43ab8cdef91f7e021490d
schema:url	https://doi.org/10.1016/s0166-0934%2896%2902151-9
resource representing a document's title	Rapid diagnosis of avian infectious bronchitis virus by the polymerase chain reaction
has PubMed Central identifier	PMC7119601
has PubMed identifier	9079758
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:441459463bb273ccd5b43ab8cdef91f7e021490d#body_text
is schema:about of	named entity 'POLYMERASE CHAIN REACTION' named entity 'identified' named entity 'IBV' named entity 'Elsevier Science' named entity 'ELECTROPHORESIS' named entity 'PRIMERS' named entity 'PROCEDURE' named entity 'TISSUE' named entity 'SAMPLES' named entity 'SELECTED' named entity 'EXTRACTED' named entity 'RT-PCR ASSAY' named entity 'NUCLEOCAPSID' named entity 'DIFFERENT' named entity 'FLUIDS' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'TISSUES' named entity 'CONSERVED REGION' named entity 'AVIAN INFECTIOUS BRONCHITIS VIRUS' named entity 'CELL CULTURES' named entity 'IDENTIFIED BY' named entity 'INFECTED' named entity 'DETECTED' named entity 'SCIENCE' named entity 'SENSITIVE' named entity 'VIRAL RNA' named entity 'FLANKING' named entity '1997' named entity 'AMPLIFIED' named entity 'DIRECT' named entity 'STRAINS' named entity 'POLYMERASE CHAIN REACTION' named entity 'MATERIAL' named entity 'GENE' named entity 'TO DETECT' named entity 'NESTED' named entity 'DIRECTLY' named entity 'SEQUENCE' named entity 'BIRDS' named entity 'USING' named entity 'RAPID DIAGNOSIS' named entity 'SIMPLE' named entity 'ORDER' named entity 'SPECIFIC' named entity 'birds' named entity 'polymerase' named entity 'assay' named entity 'tissue' named entity 'developed' named entity 'chain reaction' named entity 'samples' named entity 'Elsevier Science' named entity 'nested RT-PCR' named entity 'allantoic' named entity 'infectious bronchitis virus' named entity 'tissue samples' named entity 'PCR' named entity 'polymerase chain reaction' named entity 'evaporation' named entity 'PCR' named entity 'electron microscopy' named entity 'RNA sequences' named entity 'polymerization' named entity 'embryonated eggs' named entity 'diagnostic method' named entity 'NDV' named entity 'IBV' named entity 'IBV' named entity 'RT-PCR' named entity 'IBV' named entity 'incubation' named entity 'thermal cycler' named entity 'MgCl' named entity 'VERO' named entity 'primers' named entity 'viruses' named entity 'Cetus'

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