About: Antigenic structure of the E2 glycoprotein from transmissible gastroenteritis coronavirus

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About: Antigenic structure of the E2 glycoprotein from transmissible gastroenteritis coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Antigenic structure of the E2 glycoprotein from transmissible gastroenteritis coronavirus
Creator	Enjuanes, Luis Correa, Isabel Bullido, Maria Jimhez, Gustav0 Suiic, Carlos
Source	Elsevier; Medline; PMC
abstract	Abstract The antigenic structure of transmissible gastroenteritis (TGE) virus E2 glycoprotein has been defined at three levels: antigenic sites, antigenic subsites and epitopes. Four antigenic sites (A, B, C and D) were defined by competitive radioimmunoassay (RIA) using monoclonal antibodies (MAbs) selected from 9 fusions. About 20% (197) of the hybridomas specific for TGE virus produced neutralizing MAbs specific for site A, which was one of the antigenically dominant determinants. Site A was differentiated in three antigenic subsites: a, b and c, by characterization of 11 MAb resistant (mar) mutants, that were defined by 8, 3, and 3 MAbs, respectively. These subsites were further subdivided in epitopes. A total of 11 epitopes were defined in E2 glycoprotein, eight of which were critical for virus neutralization. Neutralizing MAbs were obtained only when native virus was used to immunize mice, although to produce hybridomas mice immunizations were made with antigen in the native, denatured, or mixtures of native and denatured form. All neutralizing MAbs reacted to conformational epitopes. The antigenic structure of the E2-glycoprotein has been defined with murine MAbs, but the antigenic sites were relevant in the swine, the natural host of the virus, because porcine sera reacted against these sites. MAbs specific for TGE virus site C reacted to non-immune porcine sera. This reactivity was not directed against porcine immunoglobulins. These results indicated that TGE virus contains epitope(s) also present in some non-immunoglobulin component of porcine serum.
has issue date	1988-04-30(xsd:dateTime)
bibo:doi	10.1016/0168-1702(88)90059-7
bibo:pmid	2453977
has license	els-covid
sha1sum (hex)	45ca49b78fd033c5e37ec5482918b446f5841c93
schema:url	https://doi.org/10.1016/0168-1702%2888%2990059-7
resource representing a document's title	Antigenic structure of the E2 glycoprotein from transmissible gastroenteritis coronavirus
has PubMed Central identifier	PMC7134057
has PubMed identifier	2453977
schema:publication	Virus Research
resource representing a document's body	covid:45ca49b78fd033c5e37ec5482918b446f5841c93#body_text
is schema:about of	named entity 'porcine' named entity 'specific' named entity 'epitopes' named entity 'immunize' named entity 'fusions' named entity 'coronavirus' named entity 'EPITOPES' named entity 'TGE' named entity 'USING' named entity 'MAB' named entity 'COMPETITIVE' named entity 'ANTIGEN' named entity 'FUSIONS' named entity 'CRITICAL' named entity 'SUBDIVIDED' named entity 'USED' named entity 'RELEVANT' named entity 'CHARACTERIZATION' named entity 'REACTIVITY' named entity 'IMMUNE' named entity 'GASTROENTERITIS' named entity 'SITES' named entity 'OBTAINED' named entity 'SWINE' named entity 'MURINE' named entity 'FORM' named entity 'DOMINANT' named entity 'SITE' named entity 'NATURAL' named entity 'GLYCOPROTEIN' named entity 'STRUCTURE' named entity 'MIXTURES' named entity 'INDICATED' named entity 'HOST' named entity 'SPECIFIC' named entity '28A' named entity 'EPITOPE' named entity 'DIRECTED' named entity 'NON-' named entity 'TOTAL' named entity 'IMMUNOGLOBULINS' named entity 'STRUCTURE' named entity 'MUTANTS' named entity 'SERA' named entity 'DENATURED' named entity 'COMPONENT' named entity 'VIRUS NEUTRALIZATION' named entity '28S' named entity '20%' named entity 'PORCINE' named entity 'DIFFERENTIATED' named entity 'MICE' named entity 'PORCINE SERUM' named entity 'SELECTED' named entity 'IMMUNIZATIONS' named entity 'ABOUT' named entity 'BUT' named entity 'GLYCOPROTEIN' named entity 'THESE' named entity 'IMMUNOGLOBULIN' named entity 'RADIOIMMUNOASSAY' named entity 'NATIVE' named entity 'DEFINED' named entity 'VIRUS' named entity 'TRANSMISSIBLE GASTROENTERITIS CORONAVIRUS' named entity 'MONOCLONAL ANTIBODIES' named entity 'DETERMINANTS' named entity 'CONTAINS' named entity 'ONE OF' named entity 'LEVELS' named entity 'RESISTANT' named entity 'TO PRODUCE' named entity 'HYBRIDOMAS' named entity 'PRESENT' named entity 'RESULTS' covid:arg/45ca49b78fd033c5e37ec5482918b446f5841c93 named entity 'porcine'

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