About: Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines
Creator	Li, Jing He, Hua Cao, Chunlai Chen, Kangyue Gao, Guoquan Wei, Suzhen
source	Elsevier; Medline; PMC
abstract	Abstract Background Chinese hamster ovary (CHO) cells are the most dependable mammalian cells for the production of recombinant proteins. Replication-incompetent retroviral vector (retrovector) is an efficient tool to generate stable cell lines. Multiple copies of integrated genes by retrovector transduction results in improved recombinant protein yield. HEK-293 and their genetic derivatives are principal cells for retrovector production. Retrovectors packaged in HEK-293 cells pose a risk of infectious agent transmission, such as viruses and mycoplasmas, from serum and packaging cells. Results In this report, retrovectors were packaged in CHO cells cultured in chemically defined (CD) media. The retrovectors were then used to transduce CHO cells. This method can block potential transmission of infectious agents from serum and packaging cells. With this method, we generated glucagon-like protein-1 Fc fusion protein (GLP-1-Fc) stable expression CHO cell lines. Productivity of GLP-1-Fc can reach 3.15 g/L. The GLP-1-Fc protein produced by this method has comparable bioactivity to that of dulaglutide (Trulicity). These stable cell lines retain 95–100% of productivity after 40 days of continuous culture (~48–56 generations). Conclusions Suspension CHO cells are clean, safe, and reliable cells for retrovector packaging. Retrovectors packaged from this system could be used to generate CHO stable cell lines for recombinant protein expression. How to cite: Li J, Wei S, Cao C, et al. Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines. Electron J Biotechnol 2019;41. https://doi.org/10.1016/j.ejbt.2019.07.002.
has issue date	2019-09-30(xsd:dateTime)
bibo:doi	10.1016/j.ejbt.2019.07.002
bibo:pmid	32288149
has license	els-covid
sha1sum (hex)	485d57a331131978f3ae4ee46e4009a5eab81ce5
schema:url	https://doi.org/10.1016/j.ejbt.2019.07.002
resource representing a document's title	Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines
has PubMed Central identifier	PMC7125944
has PubMed identifier	32288149
schema:publication	Electronic Journal of Biotechnology
resource representing a document's body	covid:485d57a331131978f3ae4ee46e4009a5eab81ce5#body_text
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