About: Cytokine systems approach demonstrates differences in innate and pro-inflammatory host responses between genetically distinct MERS-CoV isolates

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About: Cytokine systems approach demonstrates differences in innate and pro-inflammatory host responses between genetically distinct MERS-CoV isolates Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Cytokine systems approach demonstrates differences in innate and pro-inflammatory host responses between genetically distinct MERS-CoV isolates
Creator	Baric, Ralph Agnihothram, Sudhakar Chang, Jean Tisoncik-Go, Jennifer Kelly, Sara Menachery, Vineet Sova, Pavel Selinger, Christian Katze, Michael Lynn Law, G
source	Medline; PMC
abstract	BACKGROUND: The recent emergence of a novel coronavirus in the Middle East (designated MERS-CoV) is a reminder of the zoonotic and pathogenic potential of emerging coronaviruses in humans. Clinical features of Middle East respiratory syndrome (MERS) include atypical pneumonia and progressive respiratory failure that is highly reminiscent of severe acute respiratory syndrome (SARS) caused by SARS-CoV. The host response is a key component of highly pathogenic respiratory virus infection. Here, we computationally analyzed gene expression changes in a human airway epithelial cell line infected with two genetically distinct MERS-CoV strains obtained from human patients, MERS-CoV SA 1 and MERS-CoV Eng 1. RESULTS: Using topological techniques, including persistence homology and filtered clustering, we performed a comparative transcriptional analysis of human Calu-3 cell host responses to the different MERS-CoV strains, with MERS-CoV Eng 1 inducing early kinetic changes, between 3 and 12 hours post infection, compared to MERS-CoV SA 1. Robust transcriptional changes distinguished the two MERS-CoV strains predominantly at the late time points. Combining statistical analysis of infection and cytokine-stimulated Calu-3 transcriptomics, we identified differential innate responses, including up-regulation of extracellular remodeling genes following MERS-CoV Eng 1 infection and differential pro-inflammatory responses. CONCLUSIONS: Through our genomics-based approach, we found topological differences in the kinetics and magnitude of the host response to MERS-CoV SA 1 and MERS-CoV Eng 1, with differential expression of innate immune and pro-inflammatory responsive genes as a result of IFN, TNF and IL-1α signaling. Predicted activation for STAT3 mediating gene expression relevant for epithelial cell-to-cell adherens and junction signaling in MERS-CoV Eng 1 infection suggest that these transcriptional differences may be the result of amino acid differences in viral proteins known to modulate innate immunity during MERS-CoV infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-1161) contains supplementary material, which is available to authorized users.
has issue date	2014-12-22(xsd:dateTime)
bibo:doi	10.1186/1471-2164-15-1161
bibo:pmid	25534508
has license	cc-by
sha1sum (hex)	49471df378964515ebc4e4f35f1248d84ac7d626
schema:url	https://doi.org/10.1186/1471-2164-15-1161
resource representing a document's title	Cytokine systems approach demonstrates differences in innate and pro-inflammatory host responses between genetically distinct MERS-CoV isolates
has PubMed Central identifier	PMC4522970
has PubMed identifier	25534508
schema:publication	BMC Genomics
resource representing a document's body	covid:49471df378964515ebc4e4f35f1248d84ac7d626#body_text
is schema:about of	named entity 'APPROACH' named entity 'human' named entity 'emerging' named entity 'responses' named entity 'time' named entity 'zoonotic' named entity 'genetically' named entity 'filtered' named entity 'MERS' named entity 'Middle East' named entity 'expression' named entity 'host' named entity 'compared' named entity 'INFLAMMATORY' named entity 'DIFFERENTIAL' named entity 'ENG' named entity 'CALU-3' named entity 'FOLLOWING' named entity 'AIRWAY EPITHELIAL CELL' named entity 'GENES' named entity 'POST' named entity 'STRAINS' named entity 'PATHOGENIC' named entity 'HUMANS' named entity 'HOST' named entity 'CYTOKINE' named entity 'INCLUDING' named entity 'FILTERED' named entity 'PERFORMED' named entity 'PRO-INFLAMMATORY RESPONSES' named entity 'UP-REGULATION' named entity 'PROGRESSIVE RESPIRATORY FAILURE' named entity 'PATIENTS' named entity 'MIDDLE EAST' named entity 'EXTRACELLULAR' named entity 'STATISTICAL ANALYSIS' named entity 'HOMOLOGY' named entity 'HOST RESPONSE' named entity 'HERE' named entity 'INFECTION' named entity 'TRANSCRIPTIONAL' named entity 'HUMAN' named entity 'IDENTIFIED' named entity 'IS A' named entity 'DISTINCT' named entity 'USING' named entity 'EMERGENCE' named entity 'RESPONSES' named entity 'RESPIRATORY VIRUS INFECTION' named entity 'DIFFERENT' named entity 'REMINDER' named entity 'KINETIC' named entity 'ROBUST' named entity 'CYTOKINE ' named entity 'RESPONSES' named entity 'COMBINING' named entity 'INFECTED' named entity 'COMPONENT' named entity 'CALU-3 CELL' named entity 'ANALYZED' named entity 'TIME' named entity 'RESULTS' named entity 'CLINICAL FEATURES' named entity 'HIGHLY' named entity 'REMODELING' named entity '12 HOURS' named entity 'SYSTEMS' named entity 'HOST' named entity 'ISOLATES' named entity 'MERS-COV' named entity 'PRO-' named entity 'SARS-COV'

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