About: Existence and characterization of allelic variants of Sao, a newly identified surface protein from Streptococcus suis

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Existence and characterization of allelic variants of Sao, a newly identified surface protein from Streptococcus suis
Creator	Yan, Jinghua Wang, Changjun Liu, Di Liu, Cuihua Gao, George Feng, Youjun Tang, Jiaqi Zheng, Feng Dong, Yaqing Ge, Junchao Ju, Ai-Ping Pan, Xiuzhen Sun, Wen
source	PMC
abstract	Surface antigen one (Sao) is a newly identified protein from the major zoonotic pathogen, Streptococcus suis. In search of functional proteins related to the pathogenesis of Chinese S. suis 2 (SS2), unexpectedly, a variant of Sao protein was obtained. To test its prevalence in S. suis, PCR assay was adopted to address the coding genes systematically. It was found that there are three allelic variants of sao gene, namely sao-S, sao-M, and sao-L based on the different lengths of the genes (∼1.5, ∼1.7, and ∼2.0 kb, respectively). These differences were determined to be caused by heterogeneity within the number of C-terminal repeat sequences (R), which had been seen as a pathogenicity-related domain in the plant pathogen, Xanthomonas oryzae. Two variants (sao-M and sao-L) were only found in SS2. All three variant proteins were prepared in vitro and their biochemical and biophysical properties were characterized. A soluble form of Sao-M protein was then used as a capture antigen to develop an enzyme-linked immunosorbent assay method to detect antibodies against SS2 in convalescent pig sera. Taken together, the results exhibit the properties of Sao proteins and provide an efficient Sao-M-based method for monitoring SS2 infection.
has issue date	2007-10-01(xsd:dateTime)
bibo:doi	10.1111/j.1574-6968.2007.00859.x
bibo:pmid	17854470
has license	no-cc
sha1sum (hex)	4a6a830e2a187d55f7cfb44b078b3a8b65081c1c
schema:url	https://doi.org/10.1111/j.1574-6968.2007.00859.x
resource representing a document's title	Existence and characterization of allelic variants of Sao, a newly identified surface protein from Streptococcus suis
has PubMed Central identifier	PMC7110054
has PubMed identifier	17854470
schema:publication	FEMS Microbiol Lett
resource representing a document's body	covid:4a6a830e2a187d55f7cfb44b078b3a8b65081c1c#body_text
is schema:about of	named entity 'prepared' named entity 'provide' named entity 'address' named entity 'repeat sequences' named entity 'biophysical' named entity 'Xanthomonas oryzae' named entity 'Sao' named entity 'protein' named entity 'THEIR' named entity 'MAJOR' named entity 'USED' named entity 'PROTEINS' named entity 'PCR ASSAY' named entity 'CAUSED BY' named entity 'INFECTION' named entity 'EFFICIENT' named entity 'GENE' named entity 'S. SUIS 2' named entity 'BIOPHYSICAL' named entity 'GENES' named entity 'METHOD' named entity 'PROTEIN ' named entity 'ITS' named entity 'SAO' named entity 'RESULTS' named entity 'STREPTOCOCCUS SUIS' named entity 'IN VITRO' named entity 'M PROTEIN' named entity 'BIOCHEMICAL' named entity 'SERA' named entity 'PIG' named entity 'ADDRESS' named entity 'NEWLY' named entity 'IDENTIFIED' named entity 'PREVALENCE' named entity 'LENGTHS' named entity 'SAO' named entity 'STREPTOCOCCUS SUIS' named entity 'NEWLY' named entity 'OBTAINED' named entity 'SURFACE PROTEIN' named entity 'CHARACTERIZATION' named entity 'PROTEIN ' named entity 'C-TERMINAL' named entity 'TO DETECT' named entity 'ANTIBODIES' named entity 'PATHOGEN' named entity 'PATHOGENICITY' named entity 'CHINESE' named entity 'FOUND' named entity 'BASED' named entity 'PLANT' named entity 'SS2' named entity 'PROVIDE' named entity 'TERMINAL REPEAT SEQUENCES' named entity '242' named entity 'EXHIBIT' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'DETERMINED' named entity 'TAKEN' named entity 'CODING' named entity 'CAPTURE' named entity 'SOLUBLE FORM' named entity 'RELATED' named entity 'PREPARED' named entity 'CHARACTERIZED' named entity 'ASSAY METHOD' named entity 'ANTIGEN' named entity 'IN S'

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