About: Development of a lateral flow device for in‐field detection and evaluation of PCR‐based diagnostic methods for Xanthomonas campestris pv. musacearum, the causal agent of banana xanthomonas wilt

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development of a lateral flow device for in‐field detection and evaluation of PCR‐based diagnostic methods for Xanthomonas campestris pv. musacearum, the causal agent of banana xanthomonas wilt Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a lateral flow device for in‐field detection and evaluation of PCR‐based diagnostic methods for Xanthomonas campestris pv. musacearum, the causal agent of banana xanthomonas wilt
Creator	Hall, J Smith, J Johnson, G Beed, F Boonham, N Grant, M Hodgetts, J Karamura, G Nakato, V Perkins, K Studholme, D
Source	PMC
abstract	Xanthomonas campestris pv. musacearum (Xcm) is the causal agent of banana xanthomonas wilt, a major threat to banana production in eastern and central Africa. The pathogen is present in very high levels within infected plants and can be transmitted by a broad range of mechanisms; therefore early specific detection is vital for effective disease management. In this study, a polyclonal antibody (pAb) was developed and deployed in a lateral flow device (LFD) format to allow rapid in‐field detection of Xcm. Published Xcm PCR assays were also independently assessed: only two assays gave specific amplification of Xcm, whilst others cross‐reacted with non‐target Xanthomonas species. Pure cultures of Xcm were used to immunize a rabbit, the IgG antibodies purified from the serum and the resulting polyclonal antibodies tested using ELISA and LFD. Testing against a wide range of bacterial species showed the pAb detected all strains of Xcm, representing isolates from seven countries and the known genetic diversity of Xcm. The pAb also detected the closely related Xanthomonas axonopodis pv. vasculorum (Xav), primarily a sugarcane pathogen. Detection was successful in both naturally and experimentally infected banana plants, and the LFD limit of detection was 10(5) cells mL(−1). Whilst the pAb is not fully specific for Xcm, Xav has never been found in banana. Therefore the LFD can be used as a first‐line screening tool to detect Xcm in the field. Testing by LFD requires no equipment, can be performed by non‐scientists and is cost‐effective. Therefore this LFD provides a vital tool to aid in the management and control of Xcm.
has issue date	2014-10-02(xsd:dateTime)
bibo:doi	10.1111/ppa.12289
bibo:pmid	32313307
has license	no-cc
sha1sum (hex)	4f639db87398de51e46ca2455248c44fe701096f
schema:url	https://doi.org/10.1111/ppa.12289
resource representing a document's title	Development of a lateral flow device for in‐field detection and evaluation of PCR‐based diagnostic methods for Xanthomonas campestris pv. musacearum, the causal agent of banana xanthomonas wilt
has PubMed Central identifier	PMC7159137
has PubMed identifier	32313307
schema:publication	Plant Pathol
resource representing a document's body	covid:4f639db87398de51e46ca2455248c44fe701096f#body_text
is schema:about of	named entity 'naturally' named entity 'assays' named entity 'antibodies' named entity 'study' named entity 'Xanthomonas axonopodis' named entity 'Xcm' named entity 'lateral' named entity 'Xanthomonas campestris' named entity 'IGG' named entity 'PCR' named entity 'BACTERIAL' named entity 'THREAT' named entity 'CELLS' named entity 'COUNTRIES' named entity 'VITAL' named entity 'pAb' named entity 'assessed' named entity 'Xcm' named entity 'specific' named entity 'performed' named entity 'format' named entity 'Xcm' named entity 'purified' named entity 'pAb' named entity 'strains' named entity 'range' named entity 'detect' named entity 'PCR' named entity 'xanthomonas' named entity 'management' named entity 'first-line' named entity 'IgG antibodies' named entity 'screening tool' named entity 'pathogen' named entity 'banana' named entity 'genetic diversity' named entity 'pathogen' named entity 'Therefore' named entity 'plant material' named entity 'NCPPB' named entity 'antibody' named entity 'gamma-Proteobacteria' named entity 'Pseudomonas marginalis' named entity 'Xav' named entity 'glasshouse' named entity 'nutrient broth' named entity 'Xanthomonas' named entity 'bacterial culture' named entity 'formalin' named entity 'Xav' named entity 'immunogen' named entity 'banana' named entity 'causal agent' named entity 'secondary antibody' named entity 'protein' named entity 'PCR' named entity 'infection' named entity 'Advisory Committee on Dangerous Pathogens' named entity 'Xav' named entity 'Biochemical tests' named entity 'banana' named entity 'morphological' named entity 'PCR' named entity 'plant pathogens' named entity 'Xanthomonas' named entity 'banana' named entity 'sugarcane' named entity 'PCR' named entity 'M110' named entity 'Tanzania' named entity 'cultivars'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software