About: A Method To Prevent SARS-CoV-2 IgM False Positives in Gold Immunochromatography and Enzyme-Linked Immunosorbent Assays

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About: A Method To Prevent SARS-CoV-2 IgM False Positives in Gold Immunochromatography and Enzyme-Linked Immunosorbent Assays Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Method To Prevent SARS-CoV-2 IgM False Positives in Gold Immunochromatography and Enzyme-Linked Immunosorbent Assays
Creator	Wang, Qiang Zhang, Bing Ma, Qiang Fang, Li Guo, Bin Du, Qin Guo, Xiaolan Guo, Yangliu Lu, Xiaolan Mu, Daiyong Zhang, Guoyuan
Source	Medline; PMC
abstract	We set out to investigate the interference factors that led to false-positive novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgM detection results using gold immunochromatography assay (GICA) and enzyme-linked immunosorbent assay (ELISA) and the corresponding solutions. GICA and ELISA were used to detect SARS-CoV-2 IgM in 86 serum samples, including 5 influenza A virus (Flu A) IgM-positive sera, 5 influenza B virus (Flu B) IgM-positive sera, 5 Mycoplasma pneumoniae IgM-positive sera, 5 Legionella pneumophila IgM-positive sera, 6 sera of HIV infection patients, 36 rheumatoid factor IgM (RF-IgM)-positive sera, 5 sera from hypertensive patients, 5 sera from diabetes mellitus patients, and 14 sera from novel coronavirus infection disease 19 (COVID-19) patients. The interference factors causing false-positive reactivity with the two methods were analyzed, and the urea dissociation test was employed to dissociate the SARS-CoV-2 IgM-positive serum using the best dissociation concentration. The two methods detected positive SARS-CoV-2 IgM in 22 mid-to-high-level-RF-IgM-positive sera and 14 sera from COVID-19 patients; the other 50 sera were negative. At a urea dissociation concentration of 6 mol/liter, SARS-CoV-2 IgM results were positive in 1 mid-to-high-level-RF-IgM-positive serum and in 14 COVID-19 patient sera detected using GICA. At a urea dissociation concentration of 4 mol/liter and with affinity index (AI) levels lower than 0.371 set to negative, SARS-CoV-2 IgM results were positive in 3 mid-to-high-level-RF-IgM-positive sera and in 14 COVID-19 patient sera detected using ELISA. The presence of RF-IgM at mid-to-high levels could lead to false-positive reactivity of SARS-CoV-2 IgM detected using GICA and ELISA, and urea dissociation tests would be helpful in reducing SARS-CoV-2 IgM false-positive results.
has issue date	2020-05-26(xsd:dateTime)
bibo:doi	10.1128/jcm.00375-20
bibo:pmid	32277023
has license	cc-by
sha1sum (hex)	504eb8f10c7707a92f820678a9d54babc8236c9c
schema:url	https://doi.org/10.1128/jcm.00375-20
resource representing a document's title	A Method To Prevent SARS-CoV-2 IgM False Positives in Gold Immunochromatography and Enzyme-Linked Immunosorbent Assays
has PubMed Central identifier	PMC7269408
has PubMed identifier	32277023
schema:publication	J Clin Microbiol
resource representing a document's body	covid:504eb8f10c7707a92f820678a9d54babc8236c9c#body_text
is schema:about of	named entity 'SARS-CoV-2' named entity 'patients' named entity 'urea' named entity 'COVID-19' named entity 'COVID-19' named entity 'employed' named entity 'SARS-CoV-2' named entity 'RESULTS' named entity 'MID' named entity 'LED' named entity 'IGM' named entity 'UREA' named entity 'solutions' named entity 'The presence' named entity 'sera' named entity 'sera' named entity 'SARS-CoV-2' named entity 'serum' named entity 'influenza B virus' named entity 'detect' named entity 'factors' named entity 'serum' named entity 'IgM' named entity 'influenza A virus' named entity 'false-positive' named entity 'sera' named entity 'liter' named entity 'IgM' named entity 'serum samples' named entity 'assay' named entity 'IgM' named entity 'IgM' named entity 'false-positive' named entity 'urea' named entity 'SARS-CoV-2' named entity 'IgM' named entity 'Flu' named entity 'IgM' named entity 'nCoV' named entity 'COVID' named entity 'cross-reaction' named entity 'IgM' named entity 'antibody' named entity 'Biotechnology' named entity 'serum' named entity 'hypertension' named entity 'IgM' named entity 'antibodies' named entity 'COVID' named entity 'NaCl' named entity 'serum' named entity 'IgM' named entity 'SARS-CoV-2' named entity 'antigen' named entity 'immune complex' named entity 'SARS-CoV-2' named entity 'acute phase' named entity 'optical density' named entity 'false-negative' named entity 'radiology' named entity 'urea' named entity 'Flu' named entity 'infection' named entity 'urea' named entity 'nucleic acid' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'Fisher's exact test' named entity 'IgM' named entity 'urea' named entity 'urea'

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