About: Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine

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About: Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine
Creator	Pan, Wei Zhao, Ping Ren, Hao Qi, Zhong-Tian Zhao, Lan-Juan Cao, Jie Ke, Jin-Shan Qin, Zhao-Lin Yu, Jian-Guo
Source	Elsevier; Medline; PMC
abstract	Abstract The nucleocapsid (N) protein of SARS-coronavirus (SARS-CoV) is the key protein for the formation of the helical nucleocapsid during virion assembly. This protein is believed to be more conserved than other proteins of the virus, such as spike and membrane glycoprotein. In this study, the N protein of SARS-CoV was expressed in Escherichia coli DH5α and identified with pooled sera from patients in the convalescence phase of SARS. A plasmid pCI-N, encoding the full-length N gene of SARS-CoV, was constructed. Expression of the N protein was observed in COS1 cells following transfection with pCI-N. The immune responses induced by intramuscular immunization with pCI-N were evaluated in a murine model. Serum anti-N immunoglobulins and splenocytes proliferative responses against N protein were observed in immunized BALB/c mice. The major immunoglobulin G subclass recognizing N protein was immunoglobulin G2a, and stimulated splenocytes secreted high levels of gamma interferon and IL-2 in response to N protein. More importantly, the immunized mice produced strong delayed-type hypersensitivity (DTH) and CD8+ CTL responses to N protein. The study shows that N protein of SARS-CoV not only is an important B cell immunogen, but also can elicit broad-based cellular immune responses. The results indicate that the N protein may be of potential value in vaccine development for specific prophylaxis and treatment against SARS.
has issue date	2005-01-05(xsd:dateTime)
bibo:doi	10.1016/j.virol.2004.10.016
bibo:pmid	15582659
has license	els-covid
sha1sum (hex)	517e4d60093973663e37dba2969be6d7e76fbd30
schema:url	https://doi.org/10.1016/j.virol.2004.10.016
resource representing a document's title	Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine
has PubMed Central identifier	PMC7111813
has PubMed identifier	15582659
schema:publication	Virology
resource representing a document's body	covid:517e4d60093973663e37dba2969be6d7e76fbd30#body_text
is schema:about of	named entity 'sera' named entity 'phase' named entity 'patients' named entity 'plasmid' named entity 'protein' named entity 'observed' named entity 'convalescence' named entity 'More' named entity 'INDUCED' named entity 'MAY BE' named entity 'CONSERVED' named entity 'FOLLOWING' named entity 'PROPHYLAXIS' named entity 'CORONAVIRUS' named entity 'PROLIFERATIVE' named entity 'EVALUATED' named entity 'SERA' named entity 'OBSERVED' named entity 'LENGTH' named entity 'INTERFERON ' named entity 'VIRUS' named entity 'POOLED' named entity 'CD8' named entity 'SECRETED' named entity 'SPECIFIC' named entity 'MAJOR' named entity 'CONVALESCENCE' named entity 'BASED' named entity 'PATIENTS' named entity 'CORONAVIRUS' named entity 'DELAYED-TYPE HYPERSENSITIVITY' named entity 'BALB' named entity 'IMMUNE RESPONSES' named entity 'DNA VACCINE' named entity 'SERUM' named entity 'GAMMA INTERFERON' named entity 'SARS-COV' named entity 'MODEL' named entity 'HELICAL' named entity 'ENCODING' named entity 'BROAD' named entity 'IMMUNE RESPONSES' named entity 'CELLULAR' named entity 'STIMULATED' named entity 'N GENE' named entity 'NUCLEOCAPSID PROTEIN' named entity 'PROTEIN ' named entity 'SARS' named entity 'NUCLEOCAPSID' named entity 'VALUE' named entity 'FORMATION' named entity 'LEVELS' named entity 'EXPRESSION' named entity 'SPIKE' named entity 'PROTEIN ' named entity 'RESULTS' named entity 'CELLS' named entity 'VIRION ASSEMBLY' named entity 'PHASE' named entity 'STRONG' named entity 'ELICIT' named entity 'IDENTIFIED' named entity 'IL-2' named entity 'HIGH' named entity 'MURINE' named entity 'BUT' named entity 'IMMUNIZATION' named entity 'SARS' named entity 'STUDY' named entity 'COS1' named entity 'IMMUNIZED' named entity 'IMMUNOGLOBULIN G SUBCLASS' named entity 'TRANSFECTION'

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