About: A Human PrM Antibody That Recognizes a Novel Cryptic Epitope on Dengue E Glycoprotein

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: A Human PrM Antibody That Recognizes a Novel Cryptic Epitope on Dengue E Glycoprotein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Human PrM Antibody That Recognizes a Novel Cryptic Epitope on Dengue E Glycoprotein
Creator	Chow, A Tan, H Chan, Ahy Lim, Apc Lok, S
Source	PMC
abstract	Dengue virus (DENV) is a major mosquito-borne pathogen infecting up to 100 million people each year; so far no effective treatment or vaccines are available. Recently, highly cross-reactive and infection-enhancing pre-membrane (prM)-specific antibodies were found to dominate the anti-DENV immune response in humans, raising concern over vaccine candidates that contain native dengue prM sequences. In this study, we have isolated a broadly cross-reactive prM-specific antibody, D29, during a screen with a non-immunized human Fab-phage library against the four serotypes of DENV. The antibody is capable of restoring the infectivity of virtually non-infectious immature DENV (imDENV) in FcγR-bearing K562 cells. Remarkably, D29 also cross-reacted with a cryptic epitope on the envelope (E) protein located to the DI/DII junction as evidenced by site-directed mutagenesis. This cryptic epitope, while inaccessible to antibody binding in a native virus particle, may become exposed if E is not properly folded. These findings suggest that generation of anti-prM antibodies that enhance DENV infection may not be completely avoided even with immunization strategies employing E protein alone or subunits of E proteins.
has issue date	2012-04-03(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0033451
bibo:pmid	22509258
has license	cc-by
sha1sum (hex)	51a9f0de2c657fd582c5ff023027833d48e4876b
schema:url	https://doi.org/10.1371/journal.pone.0033451
resource representing a document's title	A Human PrM Antibody That Recognizes a Novel Cryptic Epitope on Dengue E Glycoprotein
has PubMed Central identifier	PMC3317930
has PubMed identifier	22509258
schema:publication	PLoS One
resource representing a document's body	covid:51a9f0de2c657fd582c5ff023027833d48e4876b#body_text
is schema:about of	named entity 'DENV' named entity 'K562 cells' named entity 'study' named entity 'effective' named entity 'DII' named entity 'Dengue' named entity 'ANTIBODY' named entity 'PRM' named entity 'SCREEN' named entity 'SEROTYPES' named entity 'AVAILABLE' named entity 'EPITOPE' named entity 'CAPABLE' named entity 'MILLION' named entity '100' named entity 'HIGHLY' named entity 'SITE-DIRECTED MUTAGENESIS' named entity 'IMMUNIZED' named entity 'EXPOSED' named entity 'CROSS' named entity 'COMPLETELY' named entity 'VACCINE' named entity 'SPECIFIC ANTIBODY' named entity 'A MAJOR' named entity 'HUMANS' named entity 'GENERATION' named entity 'DENGUE VIRUS' named entity 'FAB' named entity 'JUNCTION' named entity 'MOSQUITO' named entity 'UP TO' named entity 'INFECTING' named entity 'ENHANCE' named entity 'CROSS-REACTIVE' named entity 'FAR' named entity 'ENVELOPE' named entity 'IS A' named entity 'PROTEIN ' named entity 'PROTEINS' named entity 'TREATMENT' named entity 'CRYPTIC' named entity 'E PROTEIN' named entity 'PEOPLE' named entity 'ENHANCING' named entity 'DENGUE' named entity 'PRM' named entity 'IMMUNE RESPONSE' named entity 'ANTIBODY' named entity 'K562 CELLS' named entity 'STRATEGIES' named entity 'PHAGE LIBRARY' named entity 'VIRUS PARTICLE' named entity 'GLYCOPROTEIN' named entity 'VACCINES' named entity 'NOVEL' named entity 'CRYPTIC' named entity 'EPITOPE' named entity 'DENGUE' named entity 'THESE' named entity 'PRE' named entity 'ISOLATED' named entity 'INFECTIOUS' named entity 'YEAR' named entity 'RESTORING' named entity 'FINDINGS' named entity 'SEQUENCES' named entity 'HAVE' named entity 'STUDY' named entity 'EFFECTIVE' named entity 'RECENTLY' named entity 'BORNE' named entity 'IMMUNIZATION' named entity 'D29' named entity 'ANTIBODIES' named entity 'INFECTION' named entity 'EMPLOYING' named entity 'INFECTIVITY'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software