About: Parallel global profiling of plant TOR dynamics reveals a conserved role for LARP1 in protein translation

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About: Parallel global profiling of plant TOR dynamics reveals a conserved role for LARP1 in protein translation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Parallel global profiling of plant TOR dynamics reveals a conserved role for LARP1 in protein translation
Creator	Brunkard, Jacob Leiboff, Samuel Scarpin, M
Source	BioRxiv
abstract	TARGET OF RAPAMYCIN (TOR) is a deeply conserved protein kinase that coordinates eukaryotic metabolism with nutrient availability. In mammals, TOR specifically promotes translation of ribosomal protein mRNAs when amino acids are available to support protein synthesis. The mechanisms controlling translation downstream from TOR remain contested, however, and are largely unexplored in plants. Here, we took parallel global profiling approaches to define the in planta TOR-regulated transcriptome, translatome, proteome, and phosphoproteome. We found that TOR regulates ribosome biogenesis in plants at multiple levels, but through mechanisms that do not directly depend on the canonical 5′ oligopyrimidine tract motif (5′TOP) found in mammalian ribosomal protein mRNAs. To investigate this further, we focused on a putative TOR substrate identified in our phosphoproteome: LARP1, a eukaryotic RNA-binding protein that is proposed to mediate TOR translational control of 5′TOP mRNAs in humans and that has gained increased interest because it associates with SARS-CoV-2. By conducting parallel global profiling experiments with larp1 mutants, we discovered that the TOR-LARP1 signaling axis controls 5′TOP mRNA translation in plants and defined a set of conserved eukaryotic 5′TOP mRNAs that encode cyclins, importins/karyopherins, translation elongation factors, and TCTP1, among others. We then identified novel, plant-specific 5′TOP mRNAs involved in critical biological processes, including ribosome biogenesis, chromatin remodeling, and auxin signaling. Our study illuminates the ancestral roles of the TOR-LARP1-5′TOP metabolic regulatory network and provides evolutionary context for ongoing debates about the molecular function of LARP1 in eukaryotic cells.
has issue date	2020-05-14(xsd:dateTime)
bibo:doi	10.1101/2020.05.13.094508
has license	biorxiv
sha1sum (hex)	54ea510f30c5422855a5d1e1b906d3f895606174
schema:url	https://doi.org/10.1101/2020.05.13.094508
resource representing a document's title	Parallel global profiling of plant TOR dynamics reveals a conserved role for LARP1 in protein translation
schema:publication	bioRxiv
resource representing a document's body	covid:54ea510f30c5422855a5d1e1b906d3f895606174#body_text
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